AB264253

Anti-eIF2A antibody

Name: Anti-eIF2A antibody (ab264253) | Abcam
Brand: Abcam Limited
SKU: AB264253
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Polyclonal EIF2A antibody. Suitable for IP, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human EIF2A aa 450-500.

View Alternative Names

CDA02, MSTP004, MSTP089, EIF2A, Eukaryotic translation initiation factor 2A, eIF-2A, 65 kDa eukaryotic translation initiation factor 2A

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody (AB264253)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF2A antibody (AB264253)

Immunohistochemical analysis of FFPE human colon carcinoma tissue section labelling eIF2A with ab264233 at 1/500 dilution. DAB was used a counterstain.

Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Supplier Data

Immunoprecipitation - Anti-eIF2A antibody (AB264253)

eIF2A was immunoprecipitated from 1mg of HeLa whole cell lysates using eIF2A antibodies at 3μg/reaction. Western blot was performed on the immunprecipitates using ab264254 (lane 1), ab264253 (lane 2) and a control IgG (lane 3) at 1μg/ml.

Detected with chemilimuniscence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-eIF2A antibody (ab264253)

Predicted band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human EIF2A aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

Q9BY44

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p>Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.</p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eIF2A also referred to as eukaryotic translation initiation factor 2A is a protein involved in the initiation of mRNA translation. The eIF2A protein is distinct from eIF2α despite the similarity in their names. Its molecular weight is approximately 65 kDa. Expression of eIF2A occurs mainly in the cytoplasm of cells where it participates in the protein synthesis process. eIF2A functions as a mediator that targets the initiator methionylated-tRNA to the small ribosomal subunit in a manner independent from eIF2 complex.

Biological function summary

EIF2A impacts the translation mechanism by facilitating the binding of Met-tRNAi to the ribosome under particular conditions especially during stress responses where canonical eIF2 might be inhibited. It operates independently from the larger eIF2 complex which also includes eIF2β and eIF2γ subunits. This independent activity allows eIF2A to serve as a backup mechanism for translation initiation when the normal pathway is impaired.

Pathways

Translation regulation involves interaction with cellular stress pathways and the integrated stress response (ISR). eIF2A operates within pathways that respond to physiological stresses such as nutrient deprivation and oxidative stress which trigger alterations in protein synthesis rates. eIF2A is related to the eIF2 kinase which phosphorylates the α subunit of eIF2 leading to a general reduction in translation initiation under stress allowing selective translation of stress-response proteins.

Irregularities in eIF2A function link to neurodegenerative diseases such as Alzheimer's disease and metabolic disorders like diabetes. These conditions often involve disruptions in the stress response pathways where eIF2A participates. The protein participates with others like PERK a kinase that phosphorylates eIF2α under endoplasmic reticulum stress contributing to disease-related changes in protein synthesis. By modulating translation under stress eIF2A becomes significant in these pathological contexts.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.

See full target information EIF2A

style

left-column

style

left-column

style

right-column