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AB250411

Anti-EIF2B1 antibody [EPR13894(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal EIF2B1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

EIF2BA, EIF2B1, Translation initiation factor eIF2B subunit alpha, eIF2B GDP-GTP exchange factor subunit alpha

2 Images
Western blot - Anti-EIF2B1 antibody [EPR13894(B)] - BSA and Azide free (AB250411)
  • WB

Supplier Data

Western blot - Anti-EIF2B1 antibody [EPR13894(B)] - BSA and Azide free (AB250411)

This data was developed using ab181186, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EIF2B1 antibody [EPR13894(B)] (<a href='/en-us/products/primary-antibodies/eif2b1-antibody-epr13894b-ab181186'>ab181186</a>) at 1/10000 dilution

Lane 1:

SH-SY5Y cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Western blot - Anti-EIF2B1 antibody [EPR13894(B)] - BSA and Azide free (AB250411)
  • WB

Supplier Data

Western blot - Anti-EIF2B1 antibody [EPR13894(B)] - BSA and Azide free (AB250411)

This data was developed using ab181186, the same antibody clone in a different buffer formulation.

Immunoprecipitation. ab181186 at 1/1000 labeling elF2B1 immunoprecipitated from MCF7 cell lysate using ab181186 at 1/50.

All lanes:

Western blot - Anti-EIF2B1 antibody [EPR13894(B)] (<a href='/en-us/products/primary-antibodies/eif2b1-antibody-epr13894b-ab181186'>ab181186</a>)

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13894(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250411 is the carrier-free version of ab181186.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF2B1 also known as eukaryotic translation initiation factor 2B subunit alpha functions mechanically as a regulatory subunit in the eIF2B complex. This protein has a mass of approximately 38 kDa. EIF2B1 is expressed in various tissues throughout the body including the brain heart liver and muscle. It plays a critical role in protein synthesis by regulating the exchange of GDP for GTP on the eIF2 protein which is essential for initiating translation.
Biological function summary

EIF2B1 regulates protein synthesis by modulating the activity of the eIF2B complex which is a guanine nucleotide exchange factor. This complex comprises five subunits and EIF2B1 is integral for its function. Through regulation of eIF2 EIF2B1 indirectly controls the rate of translation initiation affecting cell growth and stress response mechanisms. In neurons EIF2B1’s activity influences neuronal development and maintenance.

Pathways

EIF2B1 functions prominently in both the unfolded protein response and integrated stress response pathways. These pathways respond to cellular stress by altering translation rates and are essential for maintaining protein homeostasis. EIF2B1 interacts primarily through these pathways with other proteins such as eIF2 and ATF4 which is a transcription factor activated during stress responses.

EIF2B1 plays a significant role in disorders like Vanishing White Matter (VWM) disease. Mutations in the EIF2B1 gene can lead to VWM a genetic condition affecting the central nervous system and presenting symptoms such as ataxia and neurological deterioration. Additionally disruption of EIF2B1 function relates to stress-related disorders due to its role in stress response pathways. Through these pathways EIF2B1 associates with proteins like eIF2 which can also affect susceptibility to stress disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit (PubMed : 25858979, PubMed : 27023709, PubMed : 31048492). Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed (PubMed : 25858979, PubMed : 31048492).
See full target information EIF2B1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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