JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB96574

Anti-EIF2B2 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal EIF2B2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EIF2B2 aa 50-300.

View Alternative Names

EIF2BB, EIF2B2, Translation initiation factor eIF2B subunit beta, S20I15, S20III15, eIF2B GDP-GTP exchange factor subunit beta

1 Images
Western blot - Anti-EIF2B2 antibody (AB96574)
  • WB

Unknown

Western blot - Anti-EIF2B2 antibody (AB96574)

10% SDS PAGE

All lanes:

Western blot - Anti-EIF2B2 antibody (ab96574) at 1/1000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human EIF2B2 aa 50-300. The exact immunogen used to generate this antibody is proprietary information.

P49770

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF2B2 also known as GCD7 is a subunit of the eukaryotic translation initiation factor 2B (eIF2B) complex. It has a molecular mass of approximately 37 kDa. This protein involves in initiation of translation primarily facilitating the exchange of GDP for GTP on the eIF2 complex which is essential for protein synthesis. EIF2B2 commonly expresses in human tissues with higher levels noted in the brain.
Biological function summary

Eukaryotic translation initiation factor 2B subunit beta participates in protein synthesis regulation through its role in the eIF2B complex. EIF2B2 functions within this complex as a non-catalytic regulatory subunit affecting the guanine nucleotide exchange activity on eIF2. This regulation influences the rate of protein synthesis especially under stress conditions where proper translation is critical for cell survival.

Pathways

Translation initiation and response to cellular stress are closely connected to EIF2B2 activity. EIF2B2 links to pathways such as the integrated stress response (ISR) and the mRNA translation pathway. This connection allows eIF2B2 to interact with other proteins like eIF2 and GCN2 modulating their activities and maintaining proper cellular responses to various stressors.

Mutations or dysfunction in EIF2B2 can lead to significant health issues particularly neurological disorders. Conditions such as leukoencephalopathy with vanishing white matter (VWM) connect directly to EIF2B2 defects. Research has also correlated EIF2B2 with diseases affecting brain function as it interacts with other proteins like eIF2 which are vital for neuronal protein synthesis and maintenance.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit (PubMed : 25858979, PubMed : 27023709, PubMed : 31048492). Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed (PubMed : 25858979, PubMed : 31048492).
See full target information EIF2B2
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 22:1695-1709 PubMed29444424

2018

Activity Regulates Cell Death within Cortical Interneurons through a Calcineurin-Dependent Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Rashi Priya,Mercedes Francisca Paredes,Theofanis Karayannis,Nusrath Yusuf,Xingchen Liu,Xavier Jaglin,Isabella Graef,Arturo Alvarez-Buylla,Gord Fishell
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB242148

Anti-EIF2S1 antibody [EPR23098-50]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 antibody [EPR23098-50] (AB242148)

  • 0
  • 0
Primary Antibodies

AB197587

Anti-DOHH antibody [EPR16730]

RabMAb|Recombinant|Trial Size

Immunoprecipitation - Anti-DOHH antibody [EPR16730] (AB197587)

  • 0
  • 0
Primary Antibodies

AB75836

Anti-GCN2 (phospho T899) antibody [EPR2320Y]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-GCN2 (phospho T899) antibody [EPR2320Y] (AB75836)

  • 4
  • 13
Primary Antibodies

AB225953

Anti-EIF2G/EIF2S3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2G/EIF2S3 antibody (AB225953)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com