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AB250298

Anti-EIF2B5 antibody [EPR13533] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal EIF2B5 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

EIF2BE, EIF2B5, Translation initiation factor eIF2B subunit epsilon, eIF2B GDP-GTP exchange factor subunit epsilon

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-EIF2B5 antibody [EPR13533] - BSA and Azide free (AB250298)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EIF2B5 antibody [EPR13533] - BSA and Azide free (AB250298)

This data was developed using ab181033, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of HeLa cells labeling EIF2B5 with ab181033 at 1/100 dilution. Counterstain DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2B5 antibody [EPR13533] - BSA and Azide free (AB250298)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2B5 antibody [EPR13533] - BSA and Azide free (AB250298)

This data was developed using ab181033, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling EIF2B5 with ab181033 at 1/50 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-EIF2B5 antibody [EPR13533] - BSA and Azide free (AB250298)
  • WB

Supplier Data

Western blot - Anti-EIF2B5 antibody [EPR13533] - BSA and Azide free (AB250298)

This data was developed using ab181033, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EIF2B5 antibody [EPR13533] (<a href='/en-us/products/primary-antibodies/eif2b5-antibody-epr13533-ab181033'>ab181033</a>) at 1/1000 dilution

Lane 1:

Hela cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Predicted band size: 80 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2B5 antibody [EPR13533] - BSA and Azide free (AB250298)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2B5 antibody [EPR13533] - BSA and Azide free (AB250298)

This data was developed using ab181033, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human uterus tissue labeling EIF2B5 with ab181033 at 1/50 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13533

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

ab250298 is the carrier-free version of ab181033.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF2B5 also known as eukaryotic translation initiation factor 2B subunit epsilon plays an important role in the regulation of protein synthesis. It is a catalytic subunit of the larger eIF2B complex which is responsible for guanine nucleotide exchange on eIF2. This protein has a molecular mass of approximately 85 kDa. EIF2B5 is ubiquitously expressed indicating its importance in various tissues. Its role in translation initiation is fundamental in controlling cellular responses to stress and nutrient signaling.
Biological function summary

Protein synthesis control by eIF2B5 impacts cell growth and adaptation. As an important component of the eIF2B complex which consists of five different subunits EIF2B5 directly interacts with eIF2 to facilitate GDP-GTP exchange. This process is essential for recycling eIF2 for successive rounds of translation initiation. Proper function of the eIF2B complex ensures cellular capacity to manage stress conditions such as amino acid deprivation or viral infection by modulating protein synthesis rates to conserve resources and adapt.

Pathways

EIF2B5 plays a significant role in the integrated stress response (ISR) pathway. Through its involvement in ISR it links with proteins like eIF2 and integrates signaling from different stressors. This pathway helps in adjusting the translation of specific mRNAs by phosphorylating eIF2 making EIF2B5 essential for the regulation of global and gene-specific translation. Additionally dysfunctions in its regulation may impact the mTOR pathway an important regulator of cell growth in response to nutrient availability although EIF2B5 mainly functions in ISR.

Mutations in EIF2B5 are associated with leukoencephalopathy with vanishing white matter (VWM) a rare neurological disorder. This condition links EIF2B5 dysfunction to impaired oligodendrocyte maturation and myelination in the brain. Additionally altered functioning of EIF2B5 may relate to other stress-related disorders as its regulatory role in translation impacts cell survival under stress conditions. Connections between eIF2B5 and proteins like eIF2 underline its involvement in proper neuronal function and response to metabolic changes important to understanding disorders like VWM.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit (PubMed : 25858979, PubMed : 27023709, PubMed : 31048492). Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed (PubMed : 25858979, PubMed : 31048492).
See full target information EIF2B5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

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