AB108218

Anti-EIF2D antibody

Name: Anti-EIF2D antibody (ab108218) | Abcam
Brand: Abcam Limited
SKU: AB108218
Price: 520 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal EIF2D antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human EIF2D aa 350-400.

View Alternative Names

HCA56, LGTN, EIF2D, Eukaryotic translation initiation factor 2D, eIF2d, Hepatocellular carcinoma-associated antigen 56, Ligatin

2 Images

Unknown

Immunoprecipitation - Anti-EIF2D antibody (AB108218)

ab108218 at 1 µg/ml detecting LGTN in HeLa whole cell lysate by WB following IP.

Lane 1 : IP with an antibody which recognizes an downstream epitope of LGTN
Lane 2 : ab108218 at 6 µg/mg of lysate
Lane 3 : Control IgG.

In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

Detection : Chemiluminescence with an exposure time of 30 seconds

All lanes:

Immunoprecipitation - Anti-EIF2D antibody (ab108218)

Predicted band size: 65 kDa

false

Unknown

Western blot - Anti-EIF2D antibody (AB108218)

All lanes:

Western blot - Anti-EIF2D antibody (ab108218) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 65 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human EIF2D aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

P41214

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab108218 was affinity purified using an epitope specific to EIF2D immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eukaryotic translation initiation factor 2D (EIF2D) also known as DAP1 or MCTS1 is a human protein involved in initiating translation of mRNA into proteins. It has a molecular weight of approximately 54 kDa. EIF2D is expressed in various tissues showing its involvement in diverse cellular processes. Mechanically EIF2D facilitates the recycling of ribosomal subunits ensuring efficient protein synthesis. It performs its role by helping the ribosome subunits which have dissociated after termination to be reused for new rounds of translation.

Biological function summary

EIF2D influences protein synthesis through its role in translation initiation and ribosome recycling. It may operate as a part of a complex potentially interacting with other factors involved in translation setup. This role is fundamental to maintaining cellular homeostasis and responding to cellular stress by adjusting protein production rates. EIF2D's interaction with the ribosome and its factors points to its active involvement in the complex regulation of cellular protein levels.

Pathways

EIF2D takes part in the translation initiation pathway which involves other proteins such as eIF1 and eIF2. These factors work together to ensure proper recognition of start codons on mRNA strands. EIF2D also fits into the mRNA surveillance pathway which is responsible for ensuring the fidelity of mRNA translation. The accuracy of this process is critical for proper cellular function and response to stress or damage signals.

EIF2D has associations with cancer and metabolic disorders. Its role in protein synthesis regulation impacts how cells grow and proliferate pathways often found altered in cancerous cells. EIF2D may be linked to components like mTOR which is involved in cell growth and metabolism. Additionally EIF2D's function in responding to cellular stress links it to metabolic syndromes where the shifts in protein synthesis can lead to pathological effects.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Translation initiation factor that is able to deliver tRNA to the P-site of the eukaryotic ribosome in a GTP-independent manner. The binding of Met-tRNA(I) occurs after the AUG codon finds its position in the P-site of 40S ribosomes, the situation that takes place during initiation complex formation on some specific RNAs. Its activity in tRNA binding with 40S subunits does not require the presence of the aminoacyl moiety. Possesses the unique ability to deliver non-Met (elongator) tRNAs into the P-site of the 40S subunit. In addition to its role in initiation, can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits.

See full target information EIF2D

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cells 11: PubMed35954244

2022

CHST7 Methylation Status Related to the Proliferation and Differentiation of Pituitary Adenomas.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Dong,Wenjian Shi,Yongliang Liu,Jingwu Li,Yu Zhang,Guilan Dong,Xiaoliu Dong,Hua Gao

Nature communications 12:6025 PubMed34654821

2021

A C. elegans model of C9orf72-associated ALS/FTD uncovers a conserved role for eIF2D in RAN translation.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshifumi Sonobe,Jihad Aburas,Gopinath Krishnan,Andrew C Fleming,Ghanashyam Ghadge,Priota Islam,Eleanor C Warren,Yuanzheng Gu,Mark W Kankel,André E X Brown,Evangelos Kiskinis,Tania F Gendron,Fen-Biao Gao,Raymond P Roos,Paschalis Kratsios

PLoS pathogens 15:e1007842 PubMed31199850

2019

Separate domains of G3BP promote efficient clustering of alphavirus replication complexes and recruitment of the translation initiation machinery.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin Götte,Marc D Panas,Kirsi Hellström,Lifeng Liu,Baila Samreen,Ola Larsson,Tero Ahola,Gerald M McInerney

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EIF2D antibody

Immunoprecipitation - Anti-EIF2D antibody (AB108218)

Western blot - Anti-EIF2D antibody (AB108218)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise