Rabbit Polyclonal EIF2D antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human EIF2D aa 350-400.
Images
Publications
Filter by
- Nature communications 12:60252021A C. elegans model of C9orf72-associated ALS/FTD uncovers a conserved role for eIF2D in RAN translation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshifumi Sonobe,Jihad Aburas,Gopinath Krishnan,Andrew C Fleming,Ghanashyam Ghadge,Priota Islam,Eleanor C Warren,Yuanzheng Gu,Mark W Kankel,André E X Brown,Evangelos Kiskinis,Tania F Gendron,Fen-Biao Gao,Raymond P Roos,Paschalis KratsiosPubMed 34654821
- PLoS pathogens 15:e10078422019Separate domains of G3BP promote efficient clustering of alphavirus replication complexes and recruitment of the translation initiation machinery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenjamin Götte et. al.PubMed 31199850
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human EIF2D aa 350-400. The exact immunogen used to generate this antibody is proprietary information. Database link P41214
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Bat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Elephant | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant | Dilution info - | Notes - |
Target data
Function
Translation initiation factor that is able to deliver tRNA to the P-site of the eukaryotic ribosome in a GTP-independent manner. The binding of Met-tRNA(I) occurs after the AUG codon finds its position in the P-site of 40S ribosomes, the situation that takes place during initiation complex formation on some specific RNAs. Its activity in tRNA binding with 40S subunits does not require the presence of the aminoacyl moiety. Possesses the unique ability to deliver non-Met (elongator) tRNAs into the P-site of the 40S subunit. In addition to its role in initiation, can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits.
Alternative names
HCA56, LGTN, EIF2D, Eukaryotic translation initiation factor 2D, eIF2d, Hepatocellular carcinoma-associated antigen 56, Ligatin
Recommended products
Rabbit Polyclonal EIF2D antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human EIF2D aa 350-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human EIF2D aa 350-400. The exact immunogen used to generate this antibody is proprietary information. Database link P41214
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab108218 was affinity purified using an epitope specific to EIF2D immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The eukaryotic translation initiation factor 2D (EIF2D) also known as DAP1 or MCTS1 is a human protein involved in initiating translation of mRNA into proteins. It has a molecular weight of approximately 54 kDa. EIF2D is expressed in various tissues showing its involvement in diverse cellular processes. Mechanically EIF2D facilitates the recycling of ribosomal subunits ensuring efficient protein synthesis. It performs its role by helping the ribosome subunits which have dissociated after termination to be reused for new rounds of translation.
- Biological function summary
EIF2D influences protein synthesis through its role in translation initiation and ribosome recycling. It may operate as a part of a complex potentially interacting with other factors involved in translation setup. This role is fundamental to maintaining cellular homeostasis and responding to cellular stress by adjusting protein production rates. EIF2D's interaction with the ribosome and its factors points to its active involvement in the complex regulation of cellular protein levels.
- Pathways
EIF2D takes part in the translation initiation pathway which involves other proteins such as eIF1 and eIF2. These factors work together to ensure proper recognition of start codons on mRNA strands. EIF2D also fits into the mRNA surveillance pathway which is responsible for ensuring the fidelity of mRNA translation. The accuracy of this process is critical for proper cellular function and response to stress or damage signals.
- Associated diseases and disorders
EIF2D has associations with cancer and metabolic disorders. Its role in protein synthesis regulation impacts how cells grow and proliferate pathways often found altered in cancerous cells. EIF2D may be linked to components like mTOR which is involved in cell growth and metabolism. Additionally EIF2D's function in responding to cellular stress links it to metabolic syndromes where the shifts in protein synthesis can lead to pathological effects.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-EIF2D antibody (ab108218)
All lanes: Western blot - Anti-EIF2D antibody (ab108218) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 65 kDa
Exposure time: 3min
Immunoprecipitation - Anti-EIF2D antibody (ab108218)
ab108218 at 1 µg/ml detecting LGTN in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an downstream epitope of LGTN
Lane 2: ab108218 at 6 µg/mg of lysate
Lane 3: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 secondsAll lanes: Immunoprecipitation - Anti-EIF2D antibody (ab108218)
Predicted band size: 65 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com