Anti-EIF2S1 antibody [EIF2S1A2B8]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EIF2S1 antibody [EIF2S1A2B8] (AB50733)

ab50733 at 1/1 dilution staining human EIF2S1 in HeLa Cells by Immunocytochemistry. A 1/200 dilution of Alexa Fluor 488 anti-mouse IgG is used as a secondary.

• WB

Unknown

Western blot - Anti-EIF2S1 antibody [EIF2S1A2B8] (AB50733)

All lanes:

Western blot - Anti-EIF2S1 antibody [EIF2S1A2B8] (ab50733) at 1/50 dilution

All lanes:

NIH3T3 whole cell lysate at 50 µg

Secondary

All lanes:

anti-Mouse IgG at 1/2500 dilution

Predicted band size: 36 kDa

Observed band size: 33 kDa

true

• WB

CiteAb

Western blot - Anti-EIF2S1 antibody [EIF2S1A2B8] (AB50733)

EIF2S1 western blot using anti-EIF2S1 antibody [EIF2S1A2B8] ab50733. Publication image and figure legend from Yu, Y., Pierciey, F. J., et al., 2013, PLoS Pathog, PubMed 23592989.

ab50733 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab50733 please see the product overview.

Introduction of Insig1-Myc inhibits HCMV growth and lipid synthesis in HCMV-infected cells.(A) The effect of PERK depletion on Insig1 levels and eIF2α phosphorylation. Human fibroblasts stably transfected with pCMV-Insig1-Myc were treated with shGFP or shPERK for three days, followed by one day serum starvation and then either mock- or HCMV-infected. Whole cell extracts were prepared for Western analysis at 48 hpi. Extracts were probed with anti-PERK, anti-Myc, anti-phospho-eIF2α, anti-total eIF2α and anti-actin. Two exposures of the Insig1-myc analysis are provided. S, short exposure; L, long exposure. (B) Introduction of exogenous Insig1-Myc inhibits HCMV growth. HF cells were treated with shGFP or shPERK for two days subsequent to transient transfection with pCMV-Insig1-Myc or the vector control (pCDNA3), then the cells were serum-starved for two hours and infected with HCMV. Viruses were harvested for titration at 72 hpi. See text for details. (C) Lipid synthesis in Insig1-Myc expressing HF cells. HF cells were treated as described in (B) prior to mock or HCMV infection. Total lipid synthesis was measured at 48 hpi.

false