Rabbit Polyclonal EIF2S1 phospho S51 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human EIF2S1 phospho S51 aa 1-100.
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- Nature aging 3:1237-12502023The YAP-TEAD complex promotes senescent cell survival by lowering endoplasmic reticulum stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarlos Anerillas,Krystyna Mazan-Mamczarz,Allison B Herman,Rachel Munk,Kwan-Wood Gabriel Lam,Miguel Calvo-Rubio,Amanda Garrido,Dimitrios Tsitsipatis,Jennifer L Martindale,Gisela Altés,Martina Rossi,Yulan Piao,Jinshui Fan,Chang-Yi Cui,Supriyo De,Kotb Abdelmohsen,Rafael de Cabo,Myriam GorospePubMed 37667102
- Research (Washington, D.C.) 6:00912023SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFengli Wang,Lingjuan Wang,Shiming Gan,Shenglei Feng,Sijin Ouyang,Xiaoli Wang,Shuiqiao YuanPubMed 37223481
- Cells 11:2022Calreticulin Shortage Results in Disturbance of Calcium Storage, Mitochondrial Disease, and Kidney Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAsima Tayyeb,Gry H Dihazi,Björn Tampe,Michael Zeisberg,Desiree Tampe,Samy Hakroush,Charlotte Bührig,Jenny Frese,Nazli Serin,Marwa Eltoweissy,Gerhard A Müller,Hassan DihaziPubMed 35456008
- Frontiers in cell and developmental biology 9:7229602021Sestrin2-Mediated Autophagy Contributes to Drug Resistance Endoplasmic Reticulum Stress in Human Osteosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhen Tang,Xinghui Wei,Tian Li,Wei Wang,Hao Wu,Hui Dong,Yichao Liu,Feilong Wei,Lei Shi,Xiaokang Li,Zheng Guo,Xin XiaoPubMed 34646824
- Cellular microbiology 23:e133342021Acrylamide inhibits vaccinia virus through vimentin-independent anti-viral granule formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer J Wood,Ian J White,Jerzy Samolej,Jason MercerPubMed 33792166
- Cell death & disease 9:11292018Nitric oxide triggers the assembly of "type II" stress granules linked to decreased cell viability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnaïs Aulas et. al.PubMed 30425239
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 48% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human EIF2S1 phospho S51 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P05198
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
Associated Products
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Target data
Function
Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (PubMed:16289705, PubMed:38340717). This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex (43S PIC) (PubMed:16289705). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex (PubMed:16289705). In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (PubMed:16289705). EIF2S1/eIF2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress: phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF2-alpha in a global protein synthesis inhibitor, leading to an attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming (PubMed:19131336, PubMed:33384352, PubMed:38340717). EIF2S1/eIF2-alpha also acts as an activator of mitophagy in response to mitochondrial damage: phosphorylation by EIF2AK1/HRI promotes relocalization to the mitochondrial surface, thereby triggering PRKN-independent mitophagy (PubMed:38340717).
Alternative names
EIF2A, EIF2S1, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, eIF2-alpha
Recommended products
Rabbit Polyclonal EIF2S1 phospho S51 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human EIF2S1 phospho S51 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 48% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human EIF2S1 phospho S51 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P05198
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
This antibody was purifed by affinity chromatography using epitope-specific phospho peptide. Non-phospho specific antibodies were removed using non-phospho peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EIF2S1 also called eIF2α is a subunit of the eukaryotic translation initiation factor 2 (eIF2) complex. This protein plays an important role in the initiation of protein synthesis by regulating the binding of the initiator methionyl-tRNA to the 40S ribosomal subunit. Its molecular weight is approximately 36 kDa. EIF2S1 is widely expressed in various tissues providing a fundamental function in protein biosynthesis across different cell types.
- Biological function summary
EIF2α acts as a regulatory component of the eIF2 complex which is central to initiating translation in eukaryotic cells. Phosphorylation of eIF2α leads to inhibition of the exchange of GDP to GTP on eIF2 effectively reducing global protein synthesis while allowing the translation of specific mRNAs involved in stress responses. The eIF2α forms part of the eIF2 complex working closely with other subunits to exert its regulatory functions.
- Pathways
EIF2α is involved in the integrated stress response (ISR) and other protein synthesis pathways. The phosphorylation state of eIF2α is important in ISR linking it to proteins such as PERK and GCN2 which respond to various stress signals. Phospho-eIF2α plays a significant role in modulating the translation response to cellular stress ensuring cells can adapt or initiate apoptosis depending on the conditions.
- Associated diseases and disorders
EIF2α connects to diseases like Alzheimer's and diabetes. Alterations in phosphorylation of eIF2α are linked to endoplasmic reticulum stress in Alzheimer's affecting protein homeostasis. Connecting to diabetes dysregulated eIF2α signaling interferes with insulin synthesis and secretion. Proteins like ATF4 also intersect with eIF2α in these diseases highlighting eIF2α's involvement in critical pathological processes.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-EIF2S1 (phospho S51) antibody (ab131505)
All lanes: Western blot - Anti-EIF2S1 (phospho S51) antibody (ab131505) at 1/500 dilution
Lane 1: K562 cell extract
Lane 2: K562 cell extract treated with IFN alpha
Predicted band size: 36 kDa
Immunocytochemistry/ Immunofluorescence - Anti-EIF2S1 (phospho S51) antibody (ab131505)
Immunofluorescence analysis of methanol fixed HeLa cells labelled with ab131505 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody (ab131505)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelled with ab131505 at 1/50 dilution (left) or ab131505 preincubated with blocking peptide (right).
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