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AB32157

Anti-EIF2S1 (phospho S51) antibody [E90]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
  • What is this?

4

(27 Reviews)

|

(371 Publications)

Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) is a rabbit monoclonal antibody detecting EIF2S1 in Western Blot, IHC-P, Dot Blot. Suitable for Human, Mouse, Neurospora crassa, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 270 publications
- Trusted since 2006

View Alternative Names

EIF2A, EIF2S1, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, eIF2-alpha

12 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)

Immunohistochemical analysis of paraffin-embedded mouse pancreatic cancer sections labeling EIF2S1 with ab32157 at 1/4000 dilution (0.37 μg/mL). Hematoxylin was used as counterstain. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Antigen retrieval was heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

Positive staining on mouse pancreatic cancer without alkaline phosphatase treatment (image A). No staining on mouse pancreatic cancer with alkaline phosphatase treatment (image B)
The section was incubated with ab32157 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)

Immunohistochemical analysis of paraffin-embedded human lung cancer sections labeling EIF2S1 with ab32157 at 1/4000 dilution (0.37 μg/mL). Hematoxylin was used as counterstain. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Antigen retrieval was heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

Positive staining on human lung cancer without alkaline phosphatase treatment (image A). No staining on human lung cancer with alkaline phosphatase treatment (image B)
The section was incubated with ab32157 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)

Immunohistochemical analysis of paraffin-embedded human liver carcinoma using ab32157 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)

ab32157 showing positive staining in Hepatocellular carcinoma tissue at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)
  • WB

Lab

Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)

Blocking buffer and concentration : 2% BSA/TBST.

Diluting buffer and concentration : 2% BSA /TBST.

Exposure time : 10 seconds

All lanes:

Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/20000 dilution

Lane 1:

Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysates at 20 µg

Lane 2:

HeLa treated with Calyculin A whole cell lysates at 20 µg

Lane 3:

HeLa treated with Calyculin A and phosphatase whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)
  • WB

Unknown

Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)

All lanes:

Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/500 dilution

All lanes:

PC12 cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>)

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)
  • WB

Lab

Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)

Blocking/Diluting buffer and concentration 5% NFDM/TBST

All lanes:

Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) at 1/1000 dilution

Lane 1:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) Whole cell lysates at 15 µg

Lane 2:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 5 ug/ml tunicamycin for 18 hours whole cell lysates at 15 µg

Lane 3:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 5 ug/ml tunicamycin for 18 hours whole cell lysates. Then the membrane was incubated with alkaline phosphatase. at 15 µg

Lane 4:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 5 ug/ml tunicamycin for 18 hours whole cell lysates. Then the membrane was incubated with lambda phosphatase. at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Exposure time: 180s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)

ab32157 showing positive staining in Colonic adenocarcinoma tissue at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)

ab32157 showing positive staining in Cervical carcinoma tissue at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)

ab32157 showing positive staining in Breast carcinoma tissue at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Dot Blot - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)
  • Dot

Unknown

Dot Blot - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)

Dot blot analysis on antigen peptide. A nitrocellulose membrane was spotted with (1) phospho-peptide and (2) non-phospho-peptide at 5, 1, and 0.1 ng, and then blotted with ab32157 at 1 : 500 dilution.

Dot Blot - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)
  • Dot

Lab

Dot Blot - Anti-EIF2S1 (phospho S51) antibody [E90] (AB32157)

Dot blot analysis of EIF2S1 (pS51) phospho peptide (Lane 1), EIF2S1 non-phospho peptide (Lane 2) with ab32157 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

  • Carrier free

    Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E90

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human, Neurospora crassa

Applications

WB, IHC-P, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>In some samples, eIF2α may appear as a doublet. This observation is consistent with reports in the literature (e.g., PMID: 19017641, PMID: 21813702).</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>In some samples, eIF2α may appear as a doublet. This observation is consistent with reports in the literature (e.g., PMID: 19017641, PMID: 21813702).</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>In some samples, eIF2α may appear as a doublet. This observation is consistent with reports in the literature (e.g., PMID: 19017641, PMID: 21813702).</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "African green monkey": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Cow": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Drosophila melanogaster": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Monkey": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Neurospora crassa": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>In some samples, eIF2α may appear as a doublet. This observation is consistent with reports in the literature (e.g., PMID: 19017641, PMID: 21813702).</p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Pig": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Plants": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Synthetic peptide - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/500", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Product details

Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Dot Blot, IHC-P and WB.

Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) was first used in a scientific publication in 1987 and has been cited over 274 times in peer reviewed journals. It's performance in Western Blot in human and mouse samples is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) has 24 independent reviews from customers.

Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) specifically detects EIF2S1 Phospho-S51 (UniProt ID: P05198; Molecular weight: 37kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).

Conjugation-ready, carrier free format available for antibody clone E90 - ab214434.

Highly-cited antibody with >350 citations. Highly specific phospho S51 EIF2S1 antibody, essential for researchers studying eukaryotic initiation factor 2 subunit 1 and protein synthesis. This antibody is crucial in stress response research, particularly in understanding translation regulation and EIF2S1 phosphorylation. It is widely used in studies of cellular stress and EIF2S1 inhibitors

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF2S1 also called eIF2α is a subunit of the eukaryotic translation initiation factor 2 (eIF2) complex. This protein plays an important role in the initiation of protein synthesis by regulating the binding of the initiator methionyl-tRNA to the 40S ribosomal subunit. Its molecular weight is approximately 36 kDa. EIF2S1 is widely expressed in various tissues providing a fundamental function in protein biosynthesis across different cell types.
Biological function summary

EIF2α acts as a regulatory component of the eIF2 complex which is central to initiating translation in eukaryotic cells. Phosphorylation of eIF2α leads to inhibition of the exchange of GDP to GTP on eIF2 effectively reducing global protein synthesis while allowing the translation of specific mRNAs involved in stress responses. The eIF2α forms part of the eIF2 complex working closely with other subunits to exert its regulatory functions.

Pathways

EIF2α is involved in the integrated stress response (ISR) and other protein synthesis pathways. The phosphorylation state of eIF2α is important in ISR linking it to proteins such as PERK and GCN2 which respond to various stress signals. Phospho-eIF2α plays a significant role in modulating the translation response to cellular stress ensuring cells can adapt or initiate apoptosis depending on the conditions.

EIF2α connects to diseases like Alzheimer's and diabetes. Alterations in phosphorylation of eIF2α are linked to endoplasmic reticulum stress in Alzheimer's affecting protein homeostasis. Connecting to diabetes dysregulated eIF2α signaling interferes with insulin synthesis and secretion. Proteins like ATF4 also intersect with eIF2α in these diseases highlighting eIF2α's involvement in critical pathological processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (PubMed : 16289705, PubMed : 38340717). This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex (43S PIC) (PubMed : 16289705). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex (PubMed : 16289705). In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (PubMed : 16289705). EIF2S1/eIF2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress : phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF2-alpha in a global protein synthesis inhibitor, leading to an attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming (PubMed : 19131336, PubMed : 33384352, PubMed : 38340717). EIF2S1/eIF2-alpha also acts as an activator of mitophagy in response to mitochondrial damage : phosphorylation by EIF2AK1/HRI promotes relocalization to the mitochondrial surface, thereby triggering PRKN-independent mitophagy (PubMed : 38340717).
See full target information EIF2S1 phospho S51
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Publications (371)

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Frontiers in pharmacology 16:1593290 PubMed40880646

2025

Mechanisms of sorafenib-induced cardiotoxicity: ER stress induces upregulation of ATF3, leading to downregulation of NDUFS1 expression and mitochondrial dysfunction.

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Ming Yan,Cheng Peng,Xueyan Lang,Yilan Li,Yao Zhang

RNA (New York, N.Y.) 31:1599-1618 PubMed40803890

2025

PACT suppresses PKR activation through dsRNA binding and dimerization, and is a therapeutic target for triple-negative breast cancer.

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Addison A Young,Isabelle G Juhler,Jackson R Pierce,Holly E Bohlin,Haley A Harper,David S Onishile,Renee N Chua,Madison E Liu,Estelle N Gardner,Bennett D Elzey,Kyle A Cottrell

Virology journal 22:244 PubMed40665364

2025

Respiratory syncytial virus (RSV) enhances translation of virus-resembling AU-rich host transcripts.

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Kyra Kerkhofs,Nicholas R Guydosh,Mark A Bayfield

Nature structural & molecular biology 32:1932-1946 PubMed40646310

2025

Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control.

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Geraldine Rodschinka,Sergio Forcelloni,Felix M Kühner,Sascha Wani,Henrick Riemenschneider,Dieter Edbauer,Andrew Behrens,Danny D Nedialkova

Cell death & disease 16:512 PubMed40645933

2025

IFNγ augments TKI efficacy by alleviating protein unfolding stress to promote GSDME-mediated pyroptosis in hepatocellular carcinoma.

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Xiaoxiao Li,Fujia Lu,Jie Zhou,Xiong Li,Yan Li,Weijie Ye,Jing Li,Liguo Yang,Shi Tang,Yuhan Zhou,Songlin Yin,Yuan Gao,Haotian Shang,Tengfei Chao,Xiang Cheng,Qian Chu,Weimin Wang

Communications biology 8:983 PubMed40603560

2025

Lysyl-tRNA synthetase orchestrates translation and hormone signaling during reproduction in Aedes aegypti mosquitoes.

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Shan Li,Xueli Wang,Haoran Lu,Alexander S Raikhel,Zhen Zou

Journal for immunotherapy of cancer 13: PubMed40480656

2025

Dexmedetomidine induces immunogenic cancer cell death and sensitizes tumors to PD-1 blockade.

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Liwei Zhao,Peng Liu,Allan Sauvat,Killian Carnet Le Provost,Jiani Liu,Andrea Checcoli,Jonathan Pol,Oliver Kepp,Guido Kroemer,Lucillia Bezu

BMC complementary medicine and therapies 25:175 PubMed40369535

2025

A fermented Mistletoe (Viscum album L.) extract elicits markers characteristic for immunogenic cell death driven by endoplasmic reticulum stress in vitro.

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Ulrike Weissenstein,Sibylle Tschumi,Bettina Leonhard,Stephan Baumgartner

Science advances 11:eadt3142 PubMed40138418

2025

The clinical antiprotozoal drug halofuginone promotes weight loss by elevating GDF15 and FGF21.

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Suowen Xu,Zhenghong Liu,Tian Tian,Wenqi Zhao,Zhihua Wang,Monan Liu,Mengyun Xu,Fanshun Zhang,Zhidan Zhang,Meijie Chen,Yanjun Yin,Meiming Su,Wenxiang Fang,Wenhao Pan,Shiyong Liu,Min-Dian Li,Peter J Little,Danielle Kamato,Songyang Zhang,Dongdong Wang,Stefan Offermanns,John R Speakman,Jianping Weng

Nature 641:1319-1328 PubMed40140574

2025

Plasticity of the mammalian integrated stress response.

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Chien-Wen Chen,David Papadopoli,Krzysztof J Szkop,Bo-Jhih Guan,Mohammed Alzahrani,Jing Wu,Raul Jobava,Mais M Asraf,Dawid Krokowski,Anastasios Vourekas,William C Merrick,Anton A Komar,Antonis E Koromilas,Myriam Gorospe,Matthew J Payea,Fangfang Wang,Benjamin L L Clayton,Paul J Tesar,Ashleigh Schaffer,Alexander Miron,Ilya Bederman,Eckhard Jankowsky,Christine Vogel,Leoš Shivaya Valášek,Jonathan D Dinman,Youwei Zhang,Boaz Tirosh,Ola Larsson,Ivan Topisirovic,Maria Hatzoglou
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