JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB214434

Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free

Be the first to review this product! Submit a review

|

(21 Publications)

Rabbit Recombinant Monoclonal EIF2S1 phospho S51 antibody. Carrier free. Suitable for Dot, IHC-P, WB and reacts with Synthetic peptide - Human, Human, Mouse, Rat, Neurospora crassa samples. Cited in 21 publications.

View Alternative Names

EIF2A, EIF2S1, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, eIF2-alpha

12 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)

This IHC data was generated using the same anti-phospho EIF2S1 Serine 51 antibody clone, E90, in a different buffer formulation (cat# ab32157).

Immunohistochemical analysis of paraffin-embedded human liver carcinoma using ab32157 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)

ab32157 showing positive staining in Hepatocellular carcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32157).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)

Immunohistochemical analysis of paraffin-embedded human lung cancer sections labeling EIF2S1 with ab32157 at 1/4000 dilution (0.37 μg/mL). Hematoxylin was used as counterstain. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Antigen retrieval was heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

Positive staining on human lung cancer without alkaline phosphatase treatment (image A). No staining on human lung cancer with alkaline phosphatase treatment (image B)
The section was incubated with ab32157 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32157).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)

Immunohistochemical analysis of paraffin-embedded mouse pancreatic cancer sections labeling EIF2S1 with ab32157 at 1/4000 dilution (0.37 μg/mL). Hematoxylin was used as counterstain. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Antigen retrieval was heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

Positive staining on mouse pancreatic cancer without alkaline phosphatase treatment (image A). No staining on mouse pancreatic cancer with alkaline phosphatase treatment (image B)
The section was incubated with ab32157 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32157).

Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)
  • WB

Lab

Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32157).

Blocking buffer and concentration : 2% BSA/TBST.

Diluting buffer and concentration : 2% BSA /TBST.

Exposure time : 10 seconds

All lanes:

Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (<a href='/en-us/products/primary-antibodies/eif2s1-phospho-s51-antibody-e90-ab32157'>ab32157</a>) at 1/20000 dilution

Lane 1:

Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysates at 20 µg

Lane 2:

HeLa treated with Calyculin A whole cell lysates at 20 µg

Lane 3:

HeLa treated with Calyculin A and phosphatase whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)
  • WB

Unknown

Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32157).

All lanes:

Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (<a href='/en-us/products/primary-antibodies/eif2s1-phospho-s51-antibody-e90-ab32157'>ab32157</a>) at 1/500 dilution

All lanes:

PC12 cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>)

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)
  • WB

Lab

Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)

Blocking/Diluting buffer and concentration 5% NFDM/TBST

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32157).

All lanes:

Western blot - Anti-EIF2S1 (phospho S51) antibody [E90] (<a href='/en-us/products/primary-antibodies/eif2s1-phospho-s51-antibody-e90-ab32157'>ab32157</a>) at 1/1000 dilution

Lane 1:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) Whole cell lysates at 15 µg

Lane 2:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 5 ug/ml tunicamycin for 18 hours whole cell lysates at 15 µg

Lane 3:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 5 ug/ml tunicamycin for 18 hours whole cell lysates. Then the membrane was incubated with alkaline phosphatase. at 15 µg

Lane 4:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 5 ug/ml tunicamycin for 18 hours whole cell lysates. Then the membrane was incubated with lambda phosphatase. at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Exposure time: 180s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)

ab32157 showing positive staining in Colonic adenocarcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32157).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)

ab32157 showing positive staining in Cervical carcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32157).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)

ab32157 showing positive staining in Breast carcinoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32157).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Dot Blot - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)
  • Dot

Unknown

Dot Blot - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)

Dot blot analysis on antigen peptide. A nitrocellulose membrane was spotted with (1) phospho-peptide and (2) non-phospho-peptide at 5, 1, and 0.1 ng, and then blotted with ab32157 at 1 : 500 dilution. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32157).

Dot Blot - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)
  • Dot

Lab

Dot Blot - Anti-EIF2S1 (phospho S51) antibody [E90] - BSA and Azide free (AB214434)

Dot blot analysis of EIF2S1 (pS51) phospho peptide (Lane 1), EIF2S1 non-phospho peptide (Lane 2) with ab32157 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32157).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E90

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human, Neurospora crassa

Applications

IHC-P, Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB50733

Anti-EIF2S1 antibody [EIF2S1A2B8]

Immunocytochemistry/ Immunofluorescence - Anti-EIF2S1 antibody [EIF2S1A2B8] (AB50733)

  • 0
  • 0
Primary Antibodies

AB137626

Anti-EIF2S1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-EIF2S1 antibody (AB137626)

  • 4
  • 2
Primary Antibodies

AB115822

Anti-EIF2S1 antibody

Western blot - Anti-EIF2S1 antibody (AB115822)

  • 0
  • 0
Primary Antibodies

AB26197

Anti-EIF2S1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-EIF2S1 antibody (AB26197)

  • 5
  • 6
Primary Antibodies

AB263951

Anti-EIF2S1 antibody [EPR23098-50] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 antibody [EPR23098-50] - BSA and Azide free (AB263951)

  • 0
  • 0
Primary Antibodies

AB242148

Anti-EIF2S1 antibody [EPR23098-50]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 antibody [EPR23098-50] (AB242148)

  • 0
  • 0
Primary Antibodies

AB131489

Anti-EIF2S1 (phospho S49) antibody

Immunocytochemistry/ Immunofluorescence - Anti-EIF2S1 (phospho S49) antibody (AB131489)

  • 0
  • 0
Primary Antibodies

AB131505

Anti-EIF2S1 (phospho S51) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S51) antibody (AB131505)

  • 0
  • 0
Primary Antibodies

AB5369

Anti-EIF2S1 antibody [EIF2a]

Western blot - Anti-EIF2S1 antibody [EIF2a] (AB5369)

  • 5
  • 4

Complementary Products

Primary Antibodies

AB169528

Anti-eIF2A antibody [EPR11042]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-eIF2A antibody [EPR11042] (AB169528)

  • 5
  • 5
Primary Antibodies

AB21685

Anti-GRP78 BiP antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody (AB21685)

  • 5
  • 71
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB11419

Anti-CHOP antibody [9C8]

KO Validated|Trial Size|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-CHOP antibody [9C8] (AB11419)

  • 4
  • 33
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>In some samples, eIF2α may appear as a doublet. This observation is consistent with reports in the literature (e.g., PMID: 19017641, PMID: 21813702).</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>In some samples, eIF2α may appear as a doublet. This observation is consistent with reports in the literature (e.g., PMID: 19017641, PMID: 21813702).</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>In some samples, eIF2α may appear as a doublet. This observation is consistent with reports in the literature (e.g., PMID: 19017641, PMID: 21813702).</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "African green monkey": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Cow": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Drosophila melanogaster": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Neurospora crassa": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>In some samples, eIF2α may appear as a doublet. This observation is consistent with reports in the literature (e.g., PMID: 19017641, PMID: 21813702).</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Pig": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Plants": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Synthetic peptide - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
style
left-column

Product details

ab214434 is the carrier-free version of ab32157.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF2S1 also called eIF2α is a subunit of the eukaryotic translation initiation factor 2 (eIF2) complex. This protein plays an important role in the initiation of protein synthesis by regulating the binding of the initiator methionyl-tRNA to the 40S ribosomal subunit. Its molecular weight is approximately 36 kDa. EIF2S1 is widely expressed in various tissues providing a fundamental function in protein biosynthesis across different cell types.
Biological function summary

EIF2α acts as a regulatory component of the eIF2 complex which is central to initiating translation in eukaryotic cells. Phosphorylation of eIF2α leads to inhibition of the exchange of GDP to GTP on eIF2 effectively reducing global protein synthesis while allowing the translation of specific mRNAs involved in stress responses. The eIF2α forms part of the eIF2 complex working closely with other subunits to exert its regulatory functions.

Pathways

EIF2α is involved in the integrated stress response (ISR) and other protein synthesis pathways. The phosphorylation state of eIF2α is important in ISR linking it to proteins such as PERK and GCN2 which respond to various stress signals. Phospho-eIF2α plays a significant role in modulating the translation response to cellular stress ensuring cells can adapt or initiate apoptosis depending on the conditions.

EIF2α connects to diseases like Alzheimer's and diabetes. Alterations in phosphorylation of eIF2α are linked to endoplasmic reticulum stress in Alzheimer's affecting protein homeostasis. Connecting to diabetes dysregulated eIF2α signaling interferes with insulin synthesis and secretion. Proteins like ATF4 also intersect with eIF2α in these diseases highlighting eIF2α's involvement in critical pathological processes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (PubMed : 16289705, PubMed : 38340717). This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex (43S PIC) (PubMed : 16289705). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex (PubMed : 16289705). In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (PubMed : 16289705). EIF2S1/eIF2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress : phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF2-alpha in a global protein synthesis inhibitor, leading to an attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming (PubMed : 19131336, PubMed : 33384352, PubMed : 38340717). EIF2S1/eIF2-alpha also acts as an activator of mitophagy in response to mitochondrial damage : phosphorylation by EIF2AK1/HRI promotes relocalization to the mitochondrial surface, thereby triggering PRKN-independent mitophagy (PubMed : 38340717).
See full target information EIF2S1 pS51
style
left-column

Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Molecular carcinogenesis 63:1783-1799 PubMed38896079

2024

ZBTB7A interferes with the RPL5-P53 feedback loop and reduces endoplasmic reticulum stress-induced apoptosis of pancreatic cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Tang,Lingling Chen,Yunli Chang,Dongyun Hang,Guoyu Chen,Ying Wang,Lingmei Feng,Ming Xu

Microbiology spectrum 10:e0150922 PubMed36326498

2022

Ubiquitination of SARS-CoV-2 ORF7a Prevents Cell Death Induced by Recruiting BclXL To Activate ER Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Liu,Yanan Fu,Yanping Huang,Feng Zeng,Jingjing Rao,Xiao Xiao,Xiaoguang Sun,Hao Jin,Jian Li,Jing Yang,Weixing Du,Long Liu

eLife 11: PubMed35507395

2022

Short senolytic or senostatic interventions rescue progression of radiation-induced frailty and premature ageing in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Edward Fielder,Tengfei Wan,Ghazaleh Alimohammadiha,Abbas Ishaq,Evon Low,B Melanie Weigand,George Kelly,Craig Parker,Brigid Griffin,Diana Jurk,Viktor I Korolchuk,Thomas von Zglinicki,Satomi Miwa

Molecular medicine reports 17:7017-7026 PubMed29568863

2018

TIPE‑2 suppresses growth and aggressiveness of hepatocellular carcinoma cells through downregulation of the phosphoinositide 3‑kinase/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Wang,Chen Chen,Shuzhi Feng,Jianli Tian

Science signaling 8:ra27 PubMed25759478

2015

Phosphorylation of eIF2α triggered by mTORC1 inhibition and PP6C activation is required for autophagy and is aberrant in PP6C-mutated melanoma.

Applications

WB

Species

Human

Jordan Wengrod,Ding Wang,Sarah Weiss,Hua Zhong,Iman Osman,Lawrence B Gardner

PloS one 9:e101643 PubMed24992504

2014

Genetic inhibition of phosphorylation of the translation initiation factor eIF2α does not block Aβ-dependent elevation of BACE1 and APP levels or reduce amyloid pathology in a mouse model of Alzheimer's disease.

Applications

WB

Species

Mouse

Katherine R Sadleir,William A Eimer,Randal J Kaufman,Pavel Osten,Robert Vassar

PloS one 9:e88863 PubMed24533157

2014

The untranslated regions of classic swine fever virus RNA trigger apoptosis.

Applications

WB

Species

Unspecified reactive species

Wei-Li Hsu,Chung-Lun Chen,Shi-Wei Huang,Chia-Chen Wu,I-Hsuan Chen,Muthukumar Nadar,Yin-Peng Su,Ching-Hsiu Tsai

Scientific reports 3:3219 PubMed24225777

2013

Zebularine inhibits tumorigenesis and stemness of colorectal cancer via p53-dependent endoplasmic reticulum stress.

Applications

WB

Species

Human

Pei-Ming Yang,Yi-Ting Lin,Chia-Tung Shun,Shan-Hu Lin,Tzu-Tang Wei,Shu-Hui Chuang,Ming-Shiang Wu,Ching-Chow Chen

Nucleic acids research 41:7683-99 PubMed23804767

2013

The eIF2α/ATF4 pathway is essential for stress-induced autophagy gene expression.

Applications

WB

Species

Mouse

Wafa B'chir,Anne-Catherine Maurin,Valérie Carraro,Julien Averous,Céline Jousse,Yuki Muranishi,Laurent Parry,Georges Stepien,Pierre Fafournoux,Alain Bruhat

Physiological genomics 45:477-86 PubMed23572537

2013

Impaired autophagy, chaperone expression, and protein synthesis in response to critical illness interventions in porcine skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Varuna C Banduseela,Yi-Wen Chen,Hanna Göransson Kultima,Holly S Norman,Sudhakar Aare,Peter Radell,Lars I Eriksson,Eric P Hoffman,Lars Larsson
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com