AB155649

Anti-EIF2S2/EIF2B antibody

Name: Anti-EIF2S2/EIF2B antibody (ab155649) | Abcam
Brand: Abcam Limited
SKU: AB155649
Price: 545 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal EIF2S2/EIF2B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EIF2S2 aa 100 to C-terminus.

View Alternative Names

EIF2B, EIF2S2, Eukaryotic translation initiation factor 2 subunit 2, Eukaryotic translation initiation factor 2 subunit beta, eIF2-beta

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-EIF2S2/EIF2B antibody (AB155649)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EIF2S2/EIF2B antibody (AB155649)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling EIF2S2/EIF2B using ab155649 at a 1/500 dilution. The image in the right panel was costained with Hoechst 33343.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S2/EIF2B antibody (AB155649)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S2/EIF2B antibody (AB155649)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human SG xenograft tissue, labeling EIF2S2/EIF2B using ab155649 at a 1/500 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S2/EIF2B antibody (AB155649)

Paraffin-embedded mouse kidney tissue labeling EIF2S2/EIF2B using ab155649 at 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 minutes.

Unknown

Western blot - Anti-EIF2S2/EIF2B antibody (AB155649)

10% SDS PAGE

All lanes:

Western blot - Anti-EIF2S2/EIF2B antibody (ab155649) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

H1299 whole cell lysate at 30 µg

Lane 4:

HeLa whole cell lysate at 30 µg

Predicted band size: 38 kDa

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Unknown

Western blot - Anti-EIF2S2/EIF2B antibody (AB155649)

10 % SDS-PAGE

All lanes:

Western blot - Anti-EIF2S2/EIF2B antibody (ab155649) at 1/2000 dilution

Lane 1:

PC12 whole cell lysate at 30 µg

Lane 2:

Rat2 whole cell lysate at 30 µg

Predicted band size: 38 kDa

false

Unknown

Western blot - Anti-EIF2S2/EIF2B antibody (AB155649)

10% SDS PAGE

All lanes:

Western blot - Anti-EIF2S2/EIF2B antibody (ab155649) at 1/1000 dilution

All lanes:

mouse liver lysate at 50 µg

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EIF2S2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P20042

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0). Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0). Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF2S2 also known as eukaryotic translation initiation factor 2 subunit 2 and EIF2B part of eukaryotic translation initiation factor 2B are key players in the initiation of protein synthesis. EIF2S2 is a 38.3 kDa protein and is expressed in various tissues including the brain liver and muscle. These factors form a complex that facilitates the exchange of GDP for GTP on the EIF2 complex important for the delivery of initiator tRNA to the ribosome.

Biological function summary

EIF2S2 and EIF2B are part of the eukaryotic translation initiation factor complex which plays a significant role in protein synthesis regulation under stress conditions. This complex senses cellular stress and decreases global protein synthesis by controlling the phosphorylation status of EIF2 allowing the selective translation of stress response proteins. This mechanism helps cells to conserve resources during unfavorable conditions while still expressing proteins necessary for survival.

Pathways

EIF2S2/EIF2B orchestrates the integrated stress response (ISR) pathway and the unfolded protein response (UPR) pathway. These pathways sense disruptions in protein folding and energy stress respectively. The phosphorylation of EIF2α related to these pathways is an important regulatory step that reduces general protein synthesis and channels the cellular machinery towards resolving stress.

Defects in EIF2S2 and EIF2B contribute to disorders such as Vanishing White Matter disease and some cancers. Vanishing White Matter disease linked to mutations in EIF2B affects brain white matter and involves the regulation of stress responses through proteins like EIF2α. In certain cancers abnormalities in the EIF2 pathway can affect cell survival and proliferation linking the protein to tumorigenesis and cancer progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (PubMed : 31836389). This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form the 43S pre-initiation complex (43S PIC). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (By similarity).

See full target information EIF2S2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 16:5829-5845 PubMed39544779

2024

Zhi Zhu Ma Ren pill relieves constipation in mice through endoplasmic reticulum stress-mediated apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Wen,Yu Zhan,Jun Li,Ling Xu,Chengzi Huang,Rong Wu,Xuegui Tang

Toxicology letters 243:98-110 PubMed26739637

2016

Progenitor-derived hepatocyte-like (B-13/H) cells metabolise 1'-hydroxyestragole to a genotoxic species via a SULT2B1-dependent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Philip M Probert,Jeremy M Palmer,Wasma Alhusainy,Aimen O Amer,Ivonne M C M Rietjens,Steven A White,David E Jones,Matthew C Wright

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EIF2S2/EIF2B antibody

Immunocytochemistry/ Immunofluorescence - Anti-EIF2S2/EIF2B antibody (AB155649)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S2/EIF2B antibody (AB155649)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S2/EIF2B antibody (AB155649)

Western blot - Anti-EIF2S2/EIF2B antibody (AB155649)

Western blot - Anti-EIF2S2/EIF2B antibody (AB155649)

Western blot - Anti-EIF2S2/EIF2B antibody (AB155649)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise