AB184549

Anti-EIF2S2/EIF2B antibody [EPR15833(B)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal EIF2S2/EIF2B antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

EIF2B, EIF2S2, Eukaryotic translation initiation factor 2 subunit 2, Eukaryotic translation initiation factor 2 subunit beta, eIF2-beta

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (AB184549)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (AB184549)

Immunofluorescent staining of 4% paraformaldehyde fixed HeLa cells labeling EIF2S2/EIF2B using ab184549 at a 1/500 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) secondary at a 1/200 dilution counterstained with Dapi.

Flow Cytometry (Intracellular) - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (AB184549)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (AB184549)

Intracellular flow cytometric analysis of2% paraformaldehyde fixed 293 cells labeling EIF2S2/EIF2B using ab184549 at a 1/160 dilution (red) or aRabbit monoclonal IgG control (green).

Secondary antibody : Goat anti rabbit IgG (FITC) secondary at a 1/150 dilution

Supplier Data

Immunoprecipitation - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (AB184549)

Western blot analysis of EIF2S2/EIF2B in immunoprecipitation pellets from Jurkat lysate. ab184549 used at a 1/50 dilution. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG used at a 1/1500 dilution. Right lane shows PBS negative control.

All lanes:

Immunoprecipitation - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549)

Predicted band size: 38 kDa

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Supplier Data

Western blot - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (AB184549)

All lanes:

Western blot - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (ab184549) at 1/20000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

293T cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

false

Carrier free

Anti-EIF2S2/EIF2B antibody [EPR15833(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15833(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IP, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40 - 1/60", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/160", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF2S2 also known as eukaryotic translation initiation factor 2 subunit 2 and EIF2B part of eukaryotic translation initiation factor 2B are key players in the initiation of protein synthesis. EIF2S2 is a 38.3 kDa protein and is expressed in various tissues including the brain liver and muscle. These factors form a complex that facilitates the exchange of GDP for GTP on the EIF2 complex important for the delivery of initiator tRNA to the ribosome.

Biological function summary

EIF2S2 and EIF2B are part of the eukaryotic translation initiation factor complex which plays a significant role in protein synthesis regulation under stress conditions. This complex senses cellular stress and decreases global protein synthesis by controlling the phosphorylation status of EIF2 allowing the selective translation of stress response proteins. This mechanism helps cells to conserve resources during unfavorable conditions while still expressing proteins necessary for survival.

Pathways

EIF2S2/EIF2B orchestrates the integrated stress response (ISR) pathway and the unfolded protein response (UPR) pathway. These pathways sense disruptions in protein folding and energy stress respectively. The phosphorylation of EIF2α related to these pathways is an important regulatory step that reduces general protein synthesis and channels the cellular machinery towards resolving stress.

Defects in EIF2S2 and EIF2B contribute to disorders such as Vanishing White Matter disease and some cancers. Vanishing White Matter disease linked to mutations in EIF2B affects brain white matter and involves the regulation of stress responses through proteins like EIF2α. In certain cancers abnormalities in the EIF2 pathway can affect cell survival and proliferation linking the protein to tumorigenesis and cancer progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (PubMed : 31836389). This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form the 43S pre-initiation complex (43S PIC). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (By similarity).

See full target information EIF2S2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Toxics 11: PubMed38133379

2023

MiR-96-5p Suppresses Progression of Arsenite-Induced Human Keratinocyte Proliferation and Malignant Transformation by Targeting Denticleless E3 Ubiquitin Protein Ligase Homolog.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Li,Qiaoshi Zhao,Jinyin Yao,Chunpeng Lv,Yanhui Gao,Dianjun Sun,Yanmei Yang

Viruses 13: PubMed34452398

2021

Inhibitors of Venezuelan Equine Encephalitis Virus Identified Based on Host Interaction Partners of Viral Non-Structural Protein 3.

Applications

Unspecified application

Species

Unspecified reactive species

Allison Bakovic,Nishank Bhalla,Farhang Alem,Catherine Campbell,Weidong Zhou,Aarthi Narayanan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EIF2S2/EIF2B antibody [EPR15833(B)]

Immunocytochemistry/ Immunofluorescence - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (AB184549)

Flow Cytometry (Intracellular) - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (AB184549)

Immunoprecipitation - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (AB184549)

Western blot - Anti-EIF2S2/EIF2B antibody [EPR15833(B)] (AB184549)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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