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AB86146

Anti-eIF3A antibody

5

(2 Reviews)

|

(10 Publications)

Rabbit Polyclonal EIF3A antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human EIF3A.

View Alternative Names

EIF3S10, KIAA0139, EIF3A, Eukaryotic translation initiation factor 3 subunit A, eIF3a, Eukaryotic translation initiation factor 3 subunit 10, eIF-3-theta, eIF3 p167, eIF3 p180, eIF3 p185

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3A antibody (AB86146)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3A antibody (AB86146)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling eIF3A with ab86146 at 1/200 (1µg/ml). Detection : DAB.

Western blot - Anti-eIF3A antibody (AB86146)
  • WB

AbReview41283****

Western blot - Anti-eIF3A antibody (AB86146)

All lanes:

Western blot - Anti-eIF3A antibody (ab86146) at 1/1000 dilution

All lanes:

Human U2OS osteosarcoma whole cell lysate at 20 µg

Secondary

All lanes:

IRDye® 800CW conjugated donkey anti-rabbit polyclonal at 1/15000 dilution

Predicted band size: 166 kDa

Observed band size: 166 kDa

false

Exposure time: 5min

This image is courtesy of an anonymous Abreview

Western blot - Anti-eIF3A antibody (AB86146)
  • WB

AbReview41310****

Western blot - Anti-eIF3A antibody (AB86146)

All lanes:

Western blot - Anti-eIF3A antibody (ab86146) at 1/1000 dilution

All lanes:

Mouse pancreatic cancer whole cell lysate at 20 µg

Secondary

All lanes:

IRDye® 680LT-conjugated donkey anti-mouse IgG polyclonal at 1/15000 dilution

Predicted band size: 166 kDa

Observed band size: 166 kDa

false

Exposure time: 10min

This image is courtesy of an anonymous Abreview

Western blot - Anti-eIF3A antibody (AB86146)
  • WB

Unknown

Western blot - Anti-eIF3A antibody (AB86146)

All lanes:

Western blot - Anti-eIF3A antibody (ab86146) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 166 kDa

true

Exposure time: 3s

Immunoprecipitation - Anti-eIF3A antibody (AB86146)
  • IP

Unknown

Immunoprecipitation - Anti-eIF3A antibody (AB86146)

Detection of Human eIF3A by Immunoprecipitation, using ab86146 at 3µg/mg lysate. Image shows immunoprecipitated eIF3A detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg, with an antibody recognising an upstream epitope in lane 1, ab86146 at 1 µg/ml in lane 2 and control IgG in lane 3. Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-eIF3A antibody (ab86146)

Predicted band size: 166 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human EIF3A. The exact immunogen used to generate this antibody is proprietary information.

Q14152

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eukaryotic translation initiation factor 3 subunit A (eIF3A) sometimes known as eIF3 p170 is an important component in the initiation of protein synthesis. This protein with a molecular mass of approximately 170 kDa plays an important role in forming the eIF3 complex which is essential for the binding of mRNA to the ribosome. eIF3A is widely expressed in various tissues indicating its fundamental role in basic cellular functions.
Biological function summary

EIF3A is a central component of the eIF3 complex which consists of multiple subunits that interact to facilitate the beginning of translation. It supports the recruitment of mRNA to the small ribosomal subunit mediating the formation of the 43S pre-initiation complex. Its activity influences multiple downstream biological processes including cell growth and proliferation highlighting its essential role in maintaining normal cellular functions.

Pathways

EIF3A is integral to the mTOR signaling pathway which is important for regulating cell growth and protein synthesis. In this pathway eIF3A works closely with proteins like mTOR and Raptor interacting to control the translation initiation machinery. Additionally eIF3A contributes to the regulation of the Wnt signaling pathway which is important for cell proliferation and differentiation.

EIF3A has significant connections with various cancer types including lung cancer and breast cancer. Its overexpression often correlates with enhanced tumor growth and poor prognosis implicating its role in oncogenic pathways. In cancerous conditions eIF3A might interact with proteins such as EGFR and c-Myc which are also key players in tumor progression and cellular transformation further emphasizing its impact on disease development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed : 17581632, PubMed : 25849773). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2 : GTP : methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed : 11169732, PubMed : 17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed : 25849773, PubMed : 27462815).. (Microbial infection) Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed : 23766293, PubMed : 24357634).. (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed : 18056426).
See full target information EIF3A
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 15:1571-1588 PubMed40370535

2025

eIF3a function in immunity and protection against severe sepsis by regulating B cell quantity and function through mA modification.

Applications

Unspecified application

Species

Unspecified reactive species

Qianying Ouyang,Jiajia Cui,Yang Wang,Ke Liu,Yan Zhan,Wei Zhuo,Juan Chen,Honghao Zhou,Chenhui Luo,Jianming Xia,Liansheng Wang,Chengxian Guo,Jianting Zhang,Zhaoqian Liu,Jiye Yin

Journal of hematology & oncology 17:7 PubMed38302992

2024

METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translation.

Applications

Unspecified application

Species

Unspecified reactive species

Meilin Xue,Lei Dong,Honghai Zhang,Yangchan Li,Kangqiang Qiu,Zhicong Zhao,Min Gao,Li Han,Anthony K N Chan,Wei Li,Keith Leung,Kitty Wang,Sheela Pangeni Pokharel,Ying Qing,Wei Liu,Xueer Wang,Lili Ren,Hongjie Bi,Lu Yang,Chao Shen,Zhenhua Chen,Laleh Melstrom,Hongzhi Li,Nikolai Timchenko,Xiaolan Deng,Wendong Huang,Steven T Rosen,Jingyan Tian,Lin Xu,Jiajie Diao,Chun-Wei Chen,Jianjun Chen,Baiyong Shen,Hao Chen,Rui Su

NAR cancer 4:zcab050 PubMed35047824

2022

High-grade ovarian cancer associated H/ACA snoRNAs promote cancer cell proliferation and survival.

Applications

Unspecified application

Species

Unspecified reactive species

Laurence Faucher-Giguère,Audrey Roy,Gabrielle Deschamps-Francoeur,Sonia Couture,Ryan M Nottingham,Alan M Lambowitz,Michelle S Scott,Sherif Abou Elela

iScience 25:103645 PubMed35005567

2022

Translational and post-translational control of human naïve versus primed pluripotency.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Chen,Xiaobing Zhang,Yisha Wang,Xinyu Chen,Wenjie Chen,Songsong Dan,Shiqi She,Weiwei Hu,Jie Dai,Jianwen Hu,Qingyi Cao,Qianyu Liu,Yinghua Huang,Baoming Qin,Bo Kang,Ying-Jie Wang

Genome medicine 13:183 PubMed34798904

2021

Genome-wide identification and characterization of circular RNA mA modification in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Ye,Weiyi Feng,Jialiang Zhang,Kaiyu Zhu,Xudong Huang,Ling Pan,Jiachun Su,Yanfen Zheng,Rui Li,Shuang Deng,Ruihong Bai,Lisha Zhuang,Lusheng Wei,Junge Deng,Mei Li,Rufu Chen,Dongxin Lin,Zhixiang Zuo,Jian Zheng

Molecular cancer 19:40 PubMed32106857

2020

mA demethylase ALKBH5 inhibits tumor growth and metastasis by reducing YTHDFs-mediated YAP expression and inhibiting miR-107/LATS2-mediated YAP activity in NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Jin,Jiwei Guo,Yan Wu,Lijuan Yang,Xiaohong Wang,Jing Du,Juanjuan Dai,Weiwei Chen,Kaikai Gong,Shuang Miao,Xuelin Li,Hongliang Sun

Molecular cell 73:775-787.e10 PubMed30642763

2019

The RNA-Binding Protein PUM2 Impairs Mitochondrial Dynamics and Mitophagy During Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Davide D'Amico,Adrienne Mottis,Francesca Potenza,Vincenzo Sorrentino,Hao Li,Mario Romani,Vera Lemos,Kristina Schoonjans,Nicola Zamboni,Graham Knott,Bernard L Schneider,Johan Auwerx

Cell 176:491-504.e21 PubMed30612740

2019

METTL13 Methylation of eEF1A Increases Translational Output to Promote Tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Liu,Simone Hausmann,Scott Moore Carlson,Mary Esmeralda Fuentes,Joel William Francis,Renjitha Pillai,Shane Michael Lofgren,Laura Hulea,Kristofferson Tandoc,Jiuwei Lu,Ami Li,Nicholas Dang Nguyen,Marcello Caporicci,Michael Paul Kim,Anirban Maitra,Huamin Wang,Ignacio Ivan Wistuba,John Anthony Porco,Michael Cory Bassik,Joshua Eric Elias,Jikui Song,Ivan Topisirovic,Capucine Van Rechem,Pawel Karol Mazur,Or Gozani

Cell death & disease 9:796 PubMed30038300

2018

METTL3 regulates WTAP protein homeostasis.

Applications

WB

Species

Human

Melissa Sorci,Zaira Ianniello,Sonia Cruciani,Simone Larivera,Lavinia Ceci Ginistrelli,Ernestina Capuano,Marcella Marchioni,Francesco Fazi,Alessandro Fatica

PloS one 8:e56272 PubMed23405269

2013

MicroRNA-122 dependent binding of Ago2 protein to hepatitis C virus RNA is associated with enhanced RNA stability and translation stimulation.

Applications

IP

Species

Human

K Dominik Conrad,Florian Giering,Corinna Erfurth,Angelina Neumann,Carmen Fehr,Gunter Meister,Michael Niepmann
View all publications
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