AB155412

Anti-eIF3A antibody - N-terminal

Name: Anti-eIF3A antibody - N-terminal (ab155412) | Abcam
Brand: Abcam Limited
SKU: AB155412

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(1 Publication)

Rabbit Polyclonal EIF3A antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EIF3A aa 100-350.

View Alternative Names

EIF3S10, KIAA0139, EIF3A, Eukaryotic translation initiation factor 3 subunit A, eIF3a, Eukaryotic translation initiation factor 3 subunit 10, eIF-3-theta, eIF3 p167, eIF3 p180, eIF3 p185

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3A antibody - N-terminal (AB155412)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3A antibody - N-terminal (AB155412)

Immunohistochemical analysis of paraffin embedded Hepatoma tissue, labeling eIF3A with ab155412 at 1/500.

Immunocytochemistry/ Immunofluorescence - Anti-eIF3A antibody - N-terminal (AB155412)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-eIF3A antibody - N-terminal (AB155412)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling EIF3A protein at cytoplasm using ab155412 at a 1/500 dilution (Green).
alpha Tubulin antibody was used at 1/1000 dilution (Red)
Hoechst 33342 was used for nuclear staining (Blue).

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-eIF3A antibody - N-terminal (AB155412)

Immunofluorescence analysis of methanol fixed A431 cells, labeling eIF3A with ab155412 at 1/200. Lower image shows costaining with Hoechst 33342.

Unknown

Western blot - Anti-eIF3A antibody - N-terminal (AB155412)

5% SDS PAGE

All lanes:

Western blot - Anti-eIF3A antibody - N-terminal (ab155412) at 1/500 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 166 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EIF3A aa 100-350. The exact immunogen used to generate this antibody is proprietary information.

Q14152

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eukaryotic translation initiation factor 3 subunit A (eIF3A) sometimes known as eIF3 p170 is an important component in the initiation of protein synthesis. This protein with a molecular mass of approximately 170 kDa plays an important role in forming the eIF3 complex which is essential for the binding of mRNA to the ribosome. eIF3A is widely expressed in various tissues indicating its fundamental role in basic cellular functions.

Biological function summary

EIF3A is a central component of the eIF3 complex which consists of multiple subunits that interact to facilitate the beginning of translation. It supports the recruitment of mRNA to the small ribosomal subunit mediating the formation of the 43S pre-initiation complex. Its activity influences multiple downstream biological processes including cell growth and proliferation highlighting its essential role in maintaining normal cellular functions.

Pathways

EIF3A is integral to the mTOR signaling pathway which is important for regulating cell growth and protein synthesis. In this pathway eIF3A works closely with proteins like mTOR and Raptor interacting to control the translation initiation machinery. Additionally eIF3A contributes to the regulation of the Wnt signaling pathway which is important for cell proliferation and differentiation.

EIF3A has significant connections with various cancer types including lung cancer and breast cancer. Its overexpression often correlates with enhanced tumor growth and poor prognosis implicating its role in oncogenic pathways. In cancerous conditions eIF3A might interact with proteins such as EGFR and c-Myc which are also key players in tumor progression and cellular transformation further emphasizing its impact on disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed : 17581632, PubMed : 25849773). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2 : GTP : methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed : 11169732, PubMed : 17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed : 25849773, PubMed : 27462815).. (Microbial infection) Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed : 23766293, PubMed : 24357634).. (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed : 18056426).

See full target information EIF3A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 9: PubMed32142410

2020

Presynaptic PTPσ regulates postsynaptic NMDA receptor function through direct adhesion-independent mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Kyungdeok Kim,Wangyong Shin,Muwon Kang,Suho Lee,Doyoun Kim,Ryeonghwa Kang,Yewon Jung,Yisul Cho,Esther Yang,Hyun Kim,Yong Chul Bae,Eunjoon Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-eIF3A antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3A antibody - N-terminal (AB155412)

Immunocytochemistry/ Immunofluorescence - Anti-eIF3A antibody - N-terminal (AB155412)

Immunocytochemistry/ Immunofluorescence - Anti-eIF3A antibody - N-terminal (AB155412)

Western blot - Anti-eIF3A antibody - N-terminal (AB155412)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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