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Rabbit Recombinant Monoclonal EIF3B antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 8 publications.


Images

Western blot - Anti-eIF3B antibody [EPR5804] (AB133601), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF3B antibody [EPR5804] (AB133601), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-eIF3B antibody [EPR5804] (AB133601), expandable thumbnail
  • OI-RD Scanning - Anti-eIF3B antibody [EPR5804] (AB133601), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Mouse
Expected
Not recommended
Predicted
Predicted
Rat
Expected
Not recommended
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815, PubMed:9388245). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:9388245). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426).

Alternative names

EIF3S9, EIF3B, Eukaryotic translation initiation factor 3 subunit B, eIF3b, Eukaryotic translation initiation factor 3 subunit 9, Prt1 homolog, eIF-3-eta, eIF3 p110, eIF3 p116, hPrt1

Recommended products

Rabbit Recombinant Monoclonal EIF3B antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5804
Purification technique
Affinity purification Protein A
Dissociation constant
5.6 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The eukaryotic initiation factor 3 subunit B (eIF3B) also known as EIF3S9 or p116 plays a central role in the initiation of translation. This protein has a mass of approximately 97 kDa and serves as an important component of the eIF3 complex important for assembling the 40S ribosomal subunit with mRNA and initiator tRNA. eIF3B is ubiquitously expressed in various tissues indicating its fundamental role in cellular protein synthesis.

Biological function summary

EIF3B functions as part of the large eIF3 complex which consists of 13 subunits. This complex is important for the recruitment of mRNA to the 40S ribosomal subunit playing an essential role in the start of translation. eIF3B directly contributes to stabilizing the interaction between mRNA and the ribosome promoting the accurate and efficient formation of the translation initiation complex. This function highlights the importance of eIF3B in regulating cellular protein levels.

Pathways

EIF3B participates in key processes of mRNA translation and is integral to the translation initiation pathway. It interacts with other translation initiation factors including eIF4F and eIF5 orchestrating the assembly and positioning of the ribosome for translation initiation. Moreover eIF3B is also involved in the mTOR signaling pathway linking nutrient sensing to protein synthesis regulation.

Associated diseases and disorders

EIF3B has been associated with various types of cancer such as breast and prostate cancer where its increased expression correlates with tumor progression and poor prognosis. The dysfunction of eIF3B affects protein synthesis and cell proliferation contributing to oncogenesis. Furthermore eIF3B's role is connected to the dysregulation of mTOR pathways which is significant in cancer development. Its interaction with other proteins like eIF4E and eIF5 signifies its impact on cancer progression pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-eIF3B antibody [EPR5804] (ab133601), expandable thumbnail

    Western blot - Anti-eIF3B antibody [EPR5804] (ab133601)

    All lanes: Western blot - Anti-eIF3B antibody [EPR5804] (ab133601) at 1/10000 dilution

    Lane 1: A431 cell lysate at 10 µg

    Lane 2: 293T cell lysate at 10 µg

    Lane 3: Jurkat cell lysate at 10 µg

    Lane 4: HeLa cell lysate at 10 µg

    Lane 5: Caco 2 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 92 kDa

    Observed band size: 116 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF3B antibody [EPR5804] (ab133601), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-eIF3B antibody [EPR5804] (ab133601)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling eIF3B with purified ab133601 at 1/500 dilution (4.1 μg/mL). Cells were fixed in 100% Methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain.

  • Flow Cytometry (Intracellular) - Anti-eIF3B antibody [EPR5804] (ab133601), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-eIF3B antibody [EPR5804] (ab133601)

    Intracellular flow cytometric analysis of permeabilized HeLa cells labelling eIF3B with ab133601 at 1/10 (red) with a Rabbit IgG control (green).

  • OI-RD Scanning - Anti-eIF3B antibody [EPR5804] (ab133601), expandable thumbnail

    OI-RD Scanning - Anti-eIF3B antibody [EPR5804] (ab133601)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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