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AB19360

Anti-EIF3C antibody

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Rabbit Polyclonal EIF3C antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human EIF3C aa 850 to C-terminus.

View Alternative Names

EIF3S8, EIF3C, Eukaryotic translation initiation factor 3 subunit C, eIF3c, Eukaryotic translation initiation factor 3 subunit 8, eIF3 p110

2 Images
Immunoprecipitation - Anti-EIF3C antibody (AB19360)
  • IP

Supplier Data

Immunoprecipitation - Anti-EIF3C antibody (AB19360)

ab19360 immunoprecipitating EIF3C in HEK293T whole cell lysate.

Lane 1 : Rabbit anti EIF3C + HEK293T whole cell lysate

Lane 2 (+) : ab19360 + HEK293T whole cell lysate.

Lane 3 (-) : Control IgG instead of ab19360 in HEK293T whole cell lysate.

For western blotting, ab19360 was used at 1μg/mL.

Detection : Chemiluminescence

All lanes:

Immunoprecipitation - Anti-EIF3C antibody (ab19360)

Predicted band size: 105 kDa

false

Exposure time: 3min

Western blot - Anti-EIF3C antibody (AB19360)
  • WB

Supplier Data

Western blot - Anti-EIF3C antibody (AB19360)

Detection : Chemiluminescence

All lanes:

Western blot - Anti-EIF3C antibody (ab19360) at 0.1 µg/mL

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

HEK293T cell lysate at 50 µg

Lane 3:

Jurkat cell lysate at 50 µg

Predicted band size: 105 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human EIF3C aa 850 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99613

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/15000 - 1/30000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF3C also known as eukaryotic translation initiation factor 3 subunit C is an important component within the eIF3 complex. It possesses a molecular weight of approximately 110 kDa. This protein is expressed in various tissues with significant levels observed in actively dividing cells such as those in the immune system and epithelial tissues. EIF3C functions as a part of the initiation factor that is involved in the binding of mRNA to the ribosome facilitating the formation of the competent 40S pre-initiation complex necessary for protein synthesis.
Biological function summary

Eukaryotic translation initiation factor 3 subunit C is integral to the multi-protein eIF3 complex. This protein complex plays a critical role in the initiation phase of eukaryotic translation. EIF3C assists in assembling the pre-initiation complex which then engages in scanning the mRNA to locate the start codon. The successful translation initiation process results from the efficient interaction of EIF3C with other components of the eIF3 complex contributing to proper cellular function and regulation.

Pathways

EIF3C is actively engaged in the translation initiation pathway and the mTOR signaling pathway. The mTOR pathway which is associated with cell growth proliferation and survival relies on EIF3C as part of the eIF3 complex to regulate protein synthesis by responding to extracellular signals such as nutrients and growth factors. EIF3C interacts closely with other translation initiation factors like eIF4E as part of these pathways reinforcing its involvement in regulating cellular metabolism and growth.

Eukaryotic translation initiation factor 3 subunit C holds significance in certain cancers and neurodegenerative disorders. EIF3C expression levels have been linked to tumorigenesis with elevated levels observed in various types of cancer including lung cancer and hepatocellular carcinoma. The altered regulation of EIF3C has connections with proteins such as p53 a well-known tumor suppressor implicating its role in cellular stress responses and oncogenesis. Additionally discrepancies in EIF3C expression may correlate with neurodegenerative disorders although the precise mechanisms remain under investigation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed : 17581632, PubMed : 25849773, PubMed : 27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2 : GTP : methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed : 17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed : 25849773).
See full target information EIF3C
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