Rabbit Polyclonal EIF3D antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EIF3D aa 200 to C-terminus.
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- Bioengineered 13:1952-19612022Overexpression of Eukaryotic translation initiation factor 3D induces stem cell-like properties and metastasis in cervix cancer by activating FAK through inhibiting degradation of GRP78.
- Proceedings of the National Academy of Sciences of the United States of America 118:2021Local translation in nuclear condensate amyloid bodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhaedra R Theodoridis,Michael Bokros,Dane Marijan,Nathan C Balukoff,Dazhi Wang,Chloe C Kirk,Taylor D Budine,Harris D Goldsmith,Miling Wang,Timothy E Audas,Stephen LeePubMed 33568529
- Journal of translational medicine 17:1672019Reduced eIF3d accelerates HIV disease progression by attenuating CD8+ T cell function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Pan et. al.PubMed 31118081
- Cancer management and research 9:539-5442017High expression of eIF3d is associated with poor prognosis in patients with gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiaqi He,Xuefei Wang,Jianhua Cai,Wei Wang,Xinyu QinPubMed 29123423
- FEBS open bio 6:1201-12102016Eukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancer.
- International journal of oncology 48:2580-902016EIF3D silencing suppresses renal cell carcinoma tumorigenesis via inducing G2/M arrest through downregulation of Cyclin B1/CDK1 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiu-Wu Pan et. al.PubMed 27035563
- RNA biology 14:553-5672016Cellular mRNA recruits the ribosome via eIF3-PABP bridge to initiate internal translation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNehal Thakor,M Duane Smith,Luc Roberts,Mame Daro Faye,Harshil Patel,Hans-Joachim Wieden,Jamie H D Cate,Martin HolcikPubMed 26828225
- Chemical biology & drug design 86:715-222015RNAi-Mediated Silencing of EIF3D Alleviates Proliferation and Migration of Glioma U251 and U87MG Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingliang Ren,Chun Zhou,Hong Liang,Xuhui Wang,Lunshan XuPubMed 25682860
- Bioscience reports 34:e001612014Lentivirus-mediated knockdown of eukaryotic translation initiation factor 3 subunit D inhibits proliferation of HCT116 colon cancer cells.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human EIF3D aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O15371
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Species Rat | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
mRNA cap-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, a complex required for several steps in the initiation of protein synthesis of a specialized repertoire of mRNAs (PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:18599441, PubMed:25849773). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). In the eIF-3 complex, EIF3D specifically recognizes and binds the 7-methylguanosine cap of a subset of mRNAs (PubMed:27462815). (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426).
Alternative names
EIF3S7, EIF3D, Eukaryotic translation initiation factor 3 subunit D, eIF3d, Eukaryotic translation initiation factor 3 subunit 7, eIF-3-zeta, eIF3 p66
Recommended products
Rabbit Polyclonal EIF3D antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EIF3D aa 200 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human EIF3D aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O15371
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EIF3D also known as eukaryotic translation initiation factor 3 subunit D is part of the EIF3 complex and plays a fundamental role in the initiation phase of protein synthesis. EIF3D has a molecular weight of approximately 66 kDa. It is expressed in various tissues with high levels observed in cells that have a high rate of protein synthesis such as those found in proliferating tissues.
- Biological function summary
EIF3D integrates into the large EIF3 complex which consists of multiple subunits and is vital for the proper assembly of the 40S ribosomal subunit. This assembly process is essential for the initiation of translation acting as a scaffold for other initiation factors. EIF3D contributes to the stabilization and binding of mRNAs to the ribosome facilitating efficient translation initiation. The protein is integral for mRNA selection and because of its interaction within the complex it influences cellular proliferation and growth.
- Pathways
EIF3D participates actively in translation initiation and mRNA surveillance pathways. These pathways regulate gene expression at the translational level maintaining the fidelity and quality control of protein synthesis. EIF3D works closely with EIF3E another subunit within the EIF3 complex to modulate these processes. Their interaction ensures the correct binding of mRNA and aids in the prevention of errors during protein synthesis.
- Associated diseases and disorders
EIF3D has been implicated in cancer due to its high expression in rapidly proliferating cells and its role in sustaining increased protein synthesis rates that tumors demand. Alterations in the expression or function of EIF3D may lead to aberrant pathways that support oncogenesis. Moreover EIF3D relates to viral infections where pathogens exploit the host's translational machinery for their replication. Proteins like EIF3A within the same complex often act in unison with EIF3D to facilitate viral protein production.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-EIF3D antibody (ab155419)
7.5 % SDS-PAGE
All lanes: Western blot - Anti-EIF3D antibody (ab155419) at 1/1000 dilution
All lanes: HeLa S3 whole cell lysate at 30 µg
Predicted band size: 64 kDa
Western blot - Anti-EIF3D antibody (ab155419)
7.5 % SDS-PAGE
All lanes: Western blot - Anti-EIF3D antibody (ab155419) at 1/5000 dilution
All lanes: Mouse liver whole cell lysate at 50 µg
Predicted band size: 64 kDa
Western blot - Anti-EIF3D antibody (ab155419)
7.5 % SDS-PAGE
All lanes: Western blot - Anti-EIF3D antibody (ab155419) at 1/3000 dilution
Lane 1: PC12 whole cell lysate at 30 µg
Lane 2: Rat2 whole cell lysate at 30 µg
Predicted band size: 64 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3D antibody (ab155419)
Immunohistochemical analysis of paraffin embedded HUH7
xenograft labeling EIF3D with ab155419 antibody at 1/100.
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