AB155419

Anti-EIF3D antibody

Name: Anti-EIF3D antibody (ab155419) | Abcam
Brand: Abcam Limited
SKU: AB155419

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(11 Publications)

Rabbit Polyclonal EIF3D antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EIF3D aa 200-550.

View Alternative Names

EIF3S7, EIF3D, Eukaryotic translation initiation factor 3 subunit D, eIF3d, Eukaryotic translation initiation factor 3 subunit 7, eIF-3-zeta, eIF3 p66

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3D antibody (AB155419)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3D antibody (AB155419)

Immunohistochemical analysis of paraffin embedded HUH7 xenograft labeling EIF3D with ab155419 antibody at 1/100.

Unknown

Western blot - Anti-EIF3D antibody (AB155419)

7.5 % SDS-PAGE

All lanes:

Western blot - Anti-EIF3D antibody (ab155419) at 1/3000 dilution

Lane 1:

PC12 whole cell lysate at 30 µg

Lane 2:

Rat2 whole cell lysate at 30 µg

Predicted band size: 64 kDa

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Western blot - Anti-EIF3D antibody (AB155419)

7.5 % SDS-PAGE

All lanes:

Western blot - Anti-EIF3D antibody (ab155419) at 1/5000 dilution

All lanes:

Mouse liver whole cell lysate at 50 µg

Predicted band size: 64 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human EIF3D aa 200-550. The exact immunogen used to generate this antibody is proprietary information.

O15371

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF3D also known as eukaryotic translation initiation factor 3 subunit D is part of the EIF3 complex and plays a fundamental role in the initiation phase of protein synthesis. EIF3D has a molecular weight of approximately 66 kDa. It is expressed in various tissues with high levels observed in cells that have a high rate of protein synthesis such as those found in proliferating tissues.

Biological function summary

EIF3D integrates into the large EIF3 complex which consists of multiple subunits and is vital for the proper assembly of the 40S ribosomal subunit. This assembly process is essential for the initiation of translation acting as a scaffold for other initiation factors. EIF3D contributes to the stabilization and binding of mRNAs to the ribosome facilitating efficient translation initiation. The protein is integral for mRNA selection and because of its interaction within the complex it influences cellular proliferation and growth.

Pathways

EIF3D participates actively in translation initiation and mRNA surveillance pathways. These pathways regulate gene expression at the translational level maintaining the fidelity and quality control of protein synthesis. EIF3D works closely with EIF3E another subunit within the EIF3 complex to modulate these processes. Their interaction ensures the correct binding of mRNA and aids in the prevention of errors during protein synthesis.

EIF3D has been implicated in cancer due to its high expression in rapidly proliferating cells and its role in sustaining increased protein synthesis rates that tumors demand. Alterations in the expression or function of EIF3D may lead to aberrant pathways that support oncogenesis. Moreover EIF3D relates to viral infections where pathogens exploit the host's translational machinery for their replication. Proteins like EIF3A within the same complex often act in unison with EIF3D to facilitate viral protein production.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

mRNA cap-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, a complex required for several steps in the initiation of protein synthesis of a specialized repertoire of mRNAs (PubMed : 27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2 : GTP : methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed : 18599441, PubMed : 25849773). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed : 25849773). In the eIF-3 complex, EIF3D specifically recognizes and binds the 7-methylguanosine cap of a subset of mRNAs (PubMed : 27462815).. (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed : 18056426).

See full target information EIF3D

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eadl5638 PubMed39018414

2024

Repression of mRNA translation initiation by GIGYF1 via disrupting the eIF3-eIF4G1 interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Jung-Hyun Choi,Jun Luo,Geoffrey G Hesketh,Shuyue Guo,Angelos Pistofidis,Reese Jalal Ladak,Yuxin An,Parisa Naeli,Tommy Alain,T Martin Schmeing,Anne-Claude Gingras,Thomas Duchaine,Xu Zhang,Nahum Sonenberg,Seyed Mehdi Jafarnejad

Journal of clinical laboratory analysis 37:e24825 PubMed36592991

2023

EIF3D promotes resistance to 5-fluorouracil in colorectal cancer through upregulating RUVBL1.

Applications

Unspecified application

Species

Unspecified reactive species

Chaobin Li,Kemei Lu,Chenggang Yang,Wenfeng Du,Zhengkai Liang

Bioengineered 13:1952-1961 PubMed35104170

2022

Overexpression of Eukaryotic translation initiation factor 3D induces stem cell-like properties and metastasis in cervix cancer by activating FAK through inhibiting degradation of GRP78.

Applications

IHC-P, WB

Species

Human, Human

Yan Zhong,Jian Lan

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33568529

2021

Local translation in nuclear condensate amyloid bodies.

Applications

Unspecified application

Species

Unspecified reactive species

Phaedra R Theodoridis,Michael Bokros,Dane Marijan,Nathan C Balukoff,Dazhi Wang,Chloe C Kirk,Taylor D Budine,Harris D Goldsmith,Miling Wang,Timothy E Audas,Stephen Lee

Journal of translational medicine 17:167 PubMed31118081

2019

Reduced eIF3d accelerates HIV disease progression by attenuating CD8+ T cell function.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Pan,Zi-Ning Zhang,Lin-Bo Yin,Ya-Jing Fu,Yong-Jun Jiang,Hong Shang

Cancer management and research 9:539-544 PubMed29123423

2017

High expression of eIF3d is associated with poor prognosis in patients with gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqi He,Xuefei Wang,Jianhua Cai,Wei Wang,Xinyu Qin

FEBS open bio 6:1201-1210 PubMed28203520

2016

Eukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancer.

Applications

IHC, WB

Species

Unspecified reactive species, Unspecified reactive species

Yaying Lin,Rongrong Zhang,Ping Zhang

International journal of oncology 48:2580-90 PubMed27035563

2016

EIF3D silencing suppresses renal cell carcinoma tumorigenesis via inducing G2/M arrest through downregulation of Cyclin B1/CDK1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiu-Wu Pan,Lu Chen,Yi Hong,Dan-Feng Xu,Xi Liu,Lin Li,Yi Huang,Li-Ming Cui,Si-Shun Gan,Qi-Wei Yang,Hai Huang,Fa-Jun Qu,Jian-Qing Ye,Lin-Hui Wang,Xin-Gang Cui

RNA biology 14:553-567 PubMed26828225

2016

Cellular mRNA recruits the ribosome via eIF3-PABP bridge to initiate internal translation.

Applications

Unspecified application

Species

Unspecified reactive species

Nehal Thakor,M Duane Smith,Luc Roberts,Mame Daro Faye,Harshil Patel,Hans-Joachim Wieden,Jamie H D Cate,Martin Holcik

Chemical biology & drug design 86:715-22 PubMed25682860

2015

RNAi-Mediated Silencing of EIF3D Alleviates Proliferation and Migration of Glioma U251 and U87MG Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mingliang Ren,Chun Zhou,Hong Liang,Xuhui Wang,Lunshan Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-EIF3D antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3D antibody (AB155419)

Western blot - Anti-EIF3D antibody (AB155419)

Western blot - Anti-EIF3D antibody (AB155419)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise