AB264228

Anti-EIF3D antibody

Name: Anti-EIF3D antibody (ab264228) | Abcam
Brand: Abcam Limited
SKU: AB264228
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal EIF3D antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EIF3D aa 150-200.

View Alternative Names

EIF3S7, EIF3D, Eukaryotic translation initiation factor 3 subunit D, eIF3d, Eukaryotic translation initiation factor 3 subunit 7, eIF-3-zeta, eIF3 p66

2 Images

Supplier Data

Immunoprecipitation - Anti-EIF3D antibody (AB264228)

EIF3D was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264228 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab264228 at 0.4 μg/ml.
Lane 1 : ab264228 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 30 secs.

All lanes:

Immunoprecipitation - Anti-EIF3D antibody (ab264228)

Predicted band size: 64 kDa

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Supplier Data

Western blot - Anti-EIF3D antibody (AB264228)

Detected with chemiluminescence with exposure time of 3 minutes.

4-20% SDS-PAGE

All lanes:

Western blot - Anti-EIF3D antibody (ab264228) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK293-T whole cell lysate at 50 µg

Predicted band size: 64 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human EIF3D aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

O15371

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF3D also known as eukaryotic translation initiation factor 3 subunit D is part of the EIF3 complex and plays a fundamental role in the initiation phase of protein synthesis. EIF3D has a molecular weight of approximately 66 kDa. It is expressed in various tissues with high levels observed in cells that have a high rate of protein synthesis such as those found in proliferating tissues.

Biological function summary

EIF3D integrates into the large EIF3 complex which consists of multiple subunits and is vital for the proper assembly of the 40S ribosomal subunit. This assembly process is essential for the initiation of translation acting as a scaffold for other initiation factors. EIF3D contributes to the stabilization and binding of mRNAs to the ribosome facilitating efficient translation initiation. The protein is integral for mRNA selection and because of its interaction within the complex it influences cellular proliferation and growth.

Pathways

EIF3D participates actively in translation initiation and mRNA surveillance pathways. These pathways regulate gene expression at the translational level maintaining the fidelity and quality control of protein synthesis. EIF3D works closely with EIF3E another subunit within the EIF3 complex to modulate these processes. Their interaction ensures the correct binding of mRNA and aids in the prevention of errors during protein synthesis.

EIF3D has been implicated in cancer due to its high expression in rapidly proliferating cells and its role in sustaining increased protein synthesis rates that tumors demand. Alterations in the expression or function of EIF3D may lead to aberrant pathways that support oncogenesis. Moreover EIF3D relates to viral infections where pathogens exploit the host's translational machinery for their replication. Proteins like EIF3A within the same complex often act in unison with EIF3D to facilitate viral protein production.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

mRNA cap-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, a complex required for several steps in the initiation of protein synthesis of a specialized repertoire of mRNAs (PubMed : 27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2 : GTP : methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed : 18599441, PubMed : 25849773). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed : 25849773). In the eIF-3 complex, EIF3D specifically recognizes and binds the 7-methylguanosine cap of a subset of mRNAs (PubMed : 27462815).. (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed : 18056426).

See full target information EIF3D

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eadl5638 PubMed39018414

2024

Repression of mRNA translation initiation by GIGYF1 via disrupting the eIF3-eIF4G1 interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Jung-Hyun Choi,Jun Luo,Geoffrey G Hesketh,Shuyue Guo,Angelos Pistofidis,Reese Jalal Ladak,Yuxin An,Parisa Naeli,Tommy Alain,T Martin Schmeing,Anne-Claude Gingras,Thomas Duchaine,Xu Zhang,Nahum Sonenberg,Seyed Mehdi Jafarnejad

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-EIF3D antibody

Immunoprecipitation - Anti-EIF3D antibody (AB264228)

Western blot - Anti-EIF3D antibody (AB264228)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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