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Rabbit Polyclonal EIF3E antibody. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human samples. Cited in 10 publications.


Images

Immunoprecipitation - Anti-eIF3e antibody (AB36766), expandable thumbnail
  • Western blot - Anti-eIF3e antibody (AB36766), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF3e antibody (AB36766), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3e antibody (AB36766), expandable thumbnail
  • Western blot - Anti-eIF3e antibody (AB36766), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Predicted
Chicken
Predicted
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Predicted
Xenopus laevis
Predicted
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Chicken, Cow, Xenopus laevis, Zebrafish

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Chicken, Cow, Xenopus laevis, Zebrafish

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Chicken, Cow, Xenopus laevis, Zebrafish

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Chicken, Cow, Xenopus laevis, Zebrafish

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway (PubMed:17468741). May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins (PubMed:17310990, PubMed:17324924).

Alternative names

Recommended products

Rabbit Polyclonal EIF3E antibody. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human samples. Cited in 10 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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5 product images

  • Immunoprecipitation - Anti-eIF3e antibody (ab36766), expandable thumbnail

    Immunoprecipitation - Anti-eIF3e antibody (ab36766)

    eIF3e was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to eIF3e and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab36766.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) ab99697).
    Band: 48kDa: eIF3e.

    All lanes: Immunoprecipitation - Anti-eIF3e antibody (ab36766)

    Predicted band size: 52 kDa

  • Western blot - Anti-eIF3e antibody (ab36766), expandable thumbnail

    Western blot - Anti-eIF3e antibody (ab36766)

    All lanes: Western blot - Anti-eIF3e antibody (ab36766) at 1 µg/mL

    Lanes 1 and 5: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Lanes 2 and 6: Western blot - Jurkat whole cell lysate (Jurkat whole cell lysate ab7899) at 10 µg

    Lanes 3 and 7: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 10 µg

    Lanes 4 and 8: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 10 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 52 kDa

    Observed band size: 40 kDa, 48 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF3e antibody (ab36766), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-eIF3e antibody (ab36766)

    ab36766 staining eIF3e in MCF7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab36766 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3e antibody (ab36766), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3e antibody (ab36766)

    IHC image of ab36766 staining in pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36766, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-eIF3e antibody (ab36766), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-eIF3e antibody (ab36766)

    All lanes: Western blot - Anti-eIF3e antibody (ab36766) at 1/1000 dilution

    Lane 1: Chicken tectum lysate at 5 µg

    Lane 2: Zebrafish lysate at 5 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit polyclonal IgG at 1/10000 dilution

    Predicted band size: 52 kDa

    Observed band size: 38 kDa, 48 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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