Rabbit Recombinant Monoclonal EIF3E antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Images
![Western blot - Anti-eIF3e antibody [EPR6878(2)] - BSA and Azide free (AB248752), expandable thumbnail](https://content.abcam.com/products/images/eif3e-antibody-epr68782-bsa-and-azide-free-ab248752--western-blot-img32418.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-eIF3e antibody [EPR6878(2)] - BSA and Azide free (AB248752)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3e antibody [EPR6878(2)] - BSA and Azide free (AB248752), expandable thumbnail](https://content.abcam.com/products/images/eif3e-antibody-epr68782-bsa-and-azide-free-ab248752--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76433.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3e antibody [EPR6878(2)] - BSA and Azide free (AB248752)")
![Immunocytochemistry/ Immunofluorescence - Anti-eIF3e antibody [EPR6878(2)] - BSA and Azide free (AB248752), expandable thumbnail](https://content.abcam.com/products/images/eif3e-antibody-epr68782-bsa-and-azide-free-ab248752--immunocytochemistry-immunofluorescence-img61144.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-eIF3e antibody [EPR6878(2)] - BSA and Azide free (AB248752)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested |
| Mouse | Predicted | Predicted | Not recommended | Predicted |
| Rat | Predicted | Predicted | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway (PubMed:17468741). May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins (PubMed:17310990, PubMed:17324924).
Alternative names
EIF3S6, INT6, EIF3E, Eukaryotic translation initiation factor 3 subunit E, eIF3e, Eukaryotic translation initiation factor 3 subunit 6, Viral integration site protein INT-6 homolog, eIF-3 p48
Recommended products
Rabbit Recombinant Monoclonal EIF3E antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6878(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248752 is the carrier-free version of Anti-eIF3e antibody [EPR6878(2)] ab134958.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EIF3e also known as eukaryotic translation initiation factor 3 subunit E is a critical component of the eIF3 complex. This protein has a mass of approximately 60 kDa and is expressed in a wide range of tissues. eIF3e plays a mechanical role in the translation initiation process facilitating the assembly of the pre-initiation complex by binding to the 40S ribosomal subunit and mRNA. It is essential for the correct positioning of mRNA in the ribosome enabling efficient start codon recognition.
- Biological function summary
EIF3e participates in translation as part of the eIF3 complex which includes other subunits like eIF3a eIF3b and eIF3c. The complex facilitates ribosomal scanning and joining of the 60S ribosomal subunit promoting efficient protein synthesis. eIF3e specifically stabilizes the interaction of the initiation complex with mRNA ensuring accurate translation initiation. It is abundantly expressed in highly proliferative tissues reflecting its role in cell growth and division regulation.
- Pathways
EIF3e plays a significant role in the mTOR signaling pathway and the Wnt signaling pathway. Both pathways regulate cell growth and proliferation which are important for development and tumorigenesis. eIF3e interacts with proteins like mTOR and beta-catenin that are integral to these pathways. It influences translation efficiency and has implications for protein synthesis regulation aligned with cellular growth cues.
- Associated diseases and disorders
EIF3e has connections with cancer and viral infections. Aberrant expression and mutations of eIF3e occur in various cancers affecting normal translation control and promoting tumorigenesis. eIF3e also relates to viral pathogenesis as some viruses hijack the host's translation machinery involving eIF3e for viral protein synthesis. These interactions can involve proteins like p53 in cancer where eIF3e's dysregulation contributes to cell cycle alterations and oncogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-eIF3e antibody [EPR6878(2)] - BSA and Azide free (ab248752), expandable thumbnail](https://content.abcam.com/products/images/eif3e-antibody-epr68782-bsa-and-azide-free-ab248752--western-blot-img32418.jpg "Western blot - Anti-eIF3e antibody [EPR6878(2)] - BSA and Azide free (ab248752)")
Western blot - Anti-eIF3e antibody [EPR6878(2)] - BSA and Azide free (ab248752)
This data was developed using Anti-eIF3e antibody [EPR6878(2)] ab134958, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-eIF3e antibody [EPR6878(2)] (Anti-eIF3e antibody [EPR6878(2)] ab134958) at 1/1000 dilution
Lane 1: Jurkat cell lysate at 10 µg
Lane 2: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg
Lane 3: BxPC-3 cell lysate at 10 µg
Lane 4: Saos 2 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 52 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3e antibody [EPR6878(2)] - BSA and Azide free (ab248752), expandable thumbnail](https://content.abcam.com/products/images/eif3e-antibody-epr68782-bsa-and-azide-free-ab248752--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img76433.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3e antibody [EPR6878(2)] - BSA and Azide free (ab248752)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3e antibody [EPR6878(2)] - BSA and Azide free (ab248752)
This data was developed using Anti-eIF3e antibody [EPR6878(2)] ab134958, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling eIF3e with Anti-eIF3e antibody [EPR6878(2)] ab134958 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-eIF3e antibody [EPR6878(2)] - BSA and Azide free (ab248752), expandable thumbnail](https://content.abcam.com/products/images/eif3e-antibody-epr68782-bsa-and-azide-free-ab248752--immunocytochemistry-immunofluorescence-img61144.jpg "Immunocytochemistry/ Immunofluorescence - Anti-eIF3e antibody [EPR6878(2)] - BSA and Azide free (ab248752)")
Immunocytochemistry/ Immunofluorescence - Anti-eIF3e antibody [EPR6878(2)] - BSA and Azide free (ab248752)
This data was developed using Anti-eIF3e antibody [EPR6878(2)] ab134958, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 293T cells labelling eIF3e with Anti-eIF3e antibody [EPR6878(2)] ab134958 at 1/250 dilution.
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