AB155475

Anti-EIF3F antibody

Name: Anti-EIF3F antibody (ab155475) | Abcam
Brand: Abcam Limited
SKU: AB155475

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(2 Publications)

Rabbit Polyclonal EIF3F antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EIF3F aa 50-350.

View Alternative Names

EIF3S5, EIF3F, Eukaryotic translation initiation factor 3 subunit F, eIF3f, Deubiquitinating enzyme eIF3f, Eukaryotic translation initiation factor 3 subunit 5, eIF-3-epsilon, eIF3 p47

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3F antibody (AB155475)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3F antibody (AB155475)

Immunohistochemical analysis of paraffin-embedded Human BT483 xenograft tissue, labeling EIF3F using ab155475 at a 1/100 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-EIF3F antibody (AB155475)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EIF3F antibody (AB155475)

Immunofluorescence analysis of methanol-fixed HeLa cells, labeling EIF3F using ab155475 at a 1/200 dilution. The image in the lower panel was costained with Hoechst 33342.

Supplier Data

Western blot - Anti-EIF3F antibody (AB155475)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-EIF3F antibody (ab155475) at 1/1000 dilution

Lane 1:

A431 whole cell lysates at 30 µg

Lane 2:

HepG2 whole cell lysates at 30 µg

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EIF3F aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

O00303

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Antigen retrieval also possible using Tris-EDTA buffer (pH8.0). Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF3F also known as eukaryotic translation initiation factor 3 subunit F is a critical component in the initiation of mRNA translation. The molecular weight of EIF3F is approximately 42 kDa. This protein is expressed in various tissues with a higher presence in rapidly dividing cells such as those in the liver and testes. EIF3F plays a mechanical role in the recruitment and assembly of other initiation factors and ribosomal units facilitating the proper positioning of the mRNA on the ribosome.

Biological function summary

EIF3F contributes to the regulation of protein synthesis by being part of the EIF3 complex which is essential for translation initiation. This complex interacts with several other initiation factors to ensure efficient ribosomal translation of mRNA into proteins. EIF3F is involved in maintaining the balance between global protein synthesis and selective translation thereby influencing cell growth and proliferation. Its interaction with ribosomal RNA and other initiation factors helps in stabilizing the pre-initiation complex.

Pathways

EIF3F is integral to the mTOR signaling pathway and the endoplasmic reticulum (ER) stress response pathway. These pathways are central to cellular responses to nutrient availability and misfolded proteins respectively. The mTOR pathway involves EIF3F in regulating cell growth and autophagy through its interaction with other proteins like TSC1/2. In the ER stress response EIF3F works alongside proteins such as ATF4 to control protein synthesis rates during stress conditions.

EIF3F shows associations with cancer and neurodegenerative diseases. In cancer aberrant EIF3F expression can lead to uncontrolled cell growth and tumor formation particularly noted in liver and prostate cancers. It interacts frequently with proteins like c-Myc which are key in oncogenic pathways. In neurodegenerative conditions disruptions in EIF3F and related proteins can affect neural cell survival with links observed in disorders such as amyotrophic lateral sclerosis (ALS). The disruption in EIF3F's role in protein synthesis pathways may contribute to neuronal damage and disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed : 17581632, PubMed : 25849773, PubMed : 27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2 : GTP : methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed : 17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed : 25849773).. Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling.

See full target information EIF3F

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International immunopharmacology 111:109151 PubMed36007390

2022

Interferon inducible porcine 2', 5'-oligoadenylate synthetase like-1 protein limits porcine reproductive and respiratory syndrome virus 2 infection via the MDA5-mediated interferon-signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Zhao,Huiyang Sha,Huawei Li,Hang Zhang,Liangzong Huang,Ruining Wang

Oncogene 39:617-636 PubMed31527668

2019

Nuclear control of lung cancer cells migration, invasion and bioenergetics by eukaryotic translation initiation factor 3F.

Applications

Unspecified application

Species

Unspecified reactive species

Pauline Esteves,Laetitia Dard,Aurélia Brillac,Christophe Hubert,Saharnaz Sarlak,Benoît Rousseau,Elodie Dumon,Julien Izotte,Marc Bonneu,Didier Lacombe,Jean-William Dupuy,Nivea Amoedo,Rodrigue Rossignol

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EIF3F antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3F antibody (AB155475)

Immunocytochemistry/ Immunofluorescence - Anti-EIF3F antibody (AB155475)

Western blot - Anti-EIF3F antibody (AB155475)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise