JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB315966

Anti-EIF3F antibody [EPR24363-61]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal EIF3F antibody. Suitable for WB and reacts with Human, Mouse samples.

View Alternative Names

EIF3S5, EIF3F, Eukaryotic translation initiation factor 3 subunit F, eIF3f, Deubiquitinating enzyme eIF3f, Eukaryotic translation initiation factor 3 subunit 5, eIF-3-epsilon, eIF3 p47

1 Images
Western blot - Anti-EIF3F antibody [EPR24363-61] (AB315966)
  • WB

Supplier Data

Western blot - Anti-EIF3F antibody [EPR24363-61] (AB315966)

ab315966 was shown to specifically react with EIF3F in NIH/3T3, HeLa and C2C12.

Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. ab315966 and Anti-Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1/1000 and 1/20000 dilutions, respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-EIF3F antibody [EPR24363-61] (ab315966) at 1/1000 dilution

Lane 1:

NIH/3T3 at 20 µg with 3% milk in TBS-0.1 % Tween® 20 (TBS-T)

Lane 2:

HeLa at 20 µg with 3% milk in TBS-0.1 % Tween® 20 (TBS-T)

Lane 3:

C2C12 at 20 µg with 3% milk in TBS-0.1 % Tween® 20 (TBS-T)

Secondary

Lanes 1 - 3:

Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed at 1/20000 dilution

Lanes 1 - 3:

Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed at 1/20000 dilution

Observed band size: 48 kDa,50 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24363-61

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF3F also known as eukaryotic translation initiation factor 3 subunit F is a critical component in the initiation of mRNA translation. The molecular weight of EIF3F is approximately 42 kDa. This protein is expressed in various tissues with a higher presence in rapidly dividing cells such as those in the liver and testes. EIF3F plays a mechanical role in the recruitment and assembly of other initiation factors and ribosomal units facilitating the proper positioning of the mRNA on the ribosome.
Biological function summary

EIF3F contributes to the regulation of protein synthesis by being part of the EIF3 complex which is essential for translation initiation. This complex interacts with several other initiation factors to ensure efficient ribosomal translation of mRNA into proteins. EIF3F is involved in maintaining the balance between global protein synthesis and selective translation thereby influencing cell growth and proliferation. Its interaction with ribosomal RNA and other initiation factors helps in stabilizing the pre-initiation complex.

Pathways

EIF3F is integral to the mTOR signaling pathway and the endoplasmic reticulum (ER) stress response pathway. These pathways are central to cellular responses to nutrient availability and misfolded proteins respectively. The mTOR pathway involves EIF3F in regulating cell growth and autophagy through its interaction with other proteins like TSC1/2. In the ER stress response EIF3F works alongside proteins such as ATF4 to control protein synthesis rates during stress conditions.

EIF3F shows associations with cancer and neurodegenerative diseases. In cancer aberrant EIF3F expression can lead to uncontrolled cell growth and tumor formation particularly noted in liver and prostate cancers. It interacts frequently with proteins like c-Myc which are key in oncogenic pathways. In neurodegenerative conditions disruptions in EIF3F and related proteins can affect neural cell survival with links observed in disorders such as amyotrophic lateral sclerosis (ALS). The disruption in EIF3F's role in protein synthesis pathways may contribute to neuronal damage and disease progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed : 17581632, PubMed : 25849773, PubMed : 27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2 : GTP : methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed : 17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed : 25849773).. Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling.
See full target information EIF3F
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB193081

Anti-MYSM1 antibody [EPR18657]

RabMAb|Recombinant|KO Validated

Western blot - Anti-MYSM1 antibody [EPR18657] (AB193081)

  • 0
  • 1
Primary Antibodies

AB134958

Anti-eIF3e antibody [EPR6878(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-eIF3e antibody [EPR6878(2)] (AB134958)

  • 0
  • 0
Primary Antibodies

AB85968

Anti-eIF3K antibody

Western blot - Anti-eIF3K antibody (AB85968)

  • 0
  • 0
Primary Antibodies

AB174438

Anti-ATP5D antibody [4D11]

Western blot - Anti-ATP5D antibody [4D11] (AB174438)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com