Anti-eIF3g antibody [EPR16146] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-eIF3g antibody [EPR16146] - BSA and Azide free (AB251075)

This data was developed using ab191422, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed 293T cells labeling eIF3g using ab191422 at a 1/80 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-eIF3g antibody [EPR16146] - BSA and Azide free (AB251075)

This data was developed using ab191422, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cells labeling eIF3g using ab191422 at a 1/150 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/200 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100. The two negative controls : Primary ab concentration (anti-EIF3G), Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/400 dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3g antibody [EPR16146] - BSA and Azide free (AB251075)

This data was developed using ab191422, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue sections labeling eIF3g using ab191422 at a 1/50 dilution. A ready to use HRP polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3g antibody [EPR16146] - BSA and Azide free (AB251075)

This data was developed using ab191422, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded rat skeletal muscle tissue sections labeling eIF3g using ab191422 at a 1/50 dilution. A ready to use HRP polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-eIF3g antibody [EPR16146] - BSA and Azide free (AB251075)

This data was developed using ab191422, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-eIF3g antibody [EPR16146] - N-terminal (<a href='/en-us/products/primary-antibodies/eif3g-antibody-epr16146-n-terminal-ab191422'>ab191422</a>) at 1/10000 dilution

Lane 1:

293T cell lysate at 20 µg

Lane 2:

Molt-4 cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 40 kDa

false

• WB

Supplier Data

Western blot - Anti-eIF3g antibody [EPR16146] - BSA and Azide free (AB251075)

This data was developed using ab191422, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-eIF3g antibody [EPR16146] - N-terminal (<a href='/en-us/products/primary-antibodies/eif3g-antibody-epr16146-n-terminal-ab191422'>ab191422</a>) at 1/1000 dilution

Lane 1:

PC-12 cell lysate at 10 µg

Lane 2:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 40 kDa

false