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AB251137

Anti-eIF3g antibody [EPR16147] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal EIF3G antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

EIF3S4, EIF3G, Eukaryotic translation initiation factor 3 subunit G, eIF3g, Eukaryotic translation initiation factor 3 RNA-binding subunit, Eukaryotic translation initiation factor 3 subunit 4, eIF-3-delta, eIF3 p42, eIF3 p44, eIF-3 RNA-binding subunit

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (AB251137)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (AB251137)

This data was developed using ab192601, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling eIF3g with ab192601 at a 1/100 dilution ( 7.0 μg/ml). As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/400 dilution. In blue DAPI staining.

Immunoprecipitation - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (AB251137)
  • IP

Supplier Data

Immunoprecipitation - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (AB251137)

This data was developed using ab192601, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation from 1) K562 cell lysate and 2) PBS, labeling eIF3g using ab192601 at 1/40 dilution and HRP-conjugated anti-rabbit IgG specific to the non-reduced form of rabbit IgG at a 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-eIF3g antibody [EPR16147] (<a href='/en-us/products/primary-antibodies/eif3g-antibody-epr16147-ab192601'>ab192601</a>)

Predicted band size: 36 kDa

true

Western blot - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (AB251137)
  • WB

Supplier Data

Western blot - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (AB251137)

This data was developed using ab192601, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-eIF3g antibody [EPR16147] (<a href='/en-us/products/primary-antibodies/eif3g-antibody-epr16147-ab192601'>ab192601</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 20 µg

Lane 2:

MOLT-4 cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 40 kDa

true

Western blot - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (AB251137)
  • WB

Supplier Data

Western blot - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (AB251137)

This data was developed using ab192601, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-eIF3g antibody [EPR16147] (<a href='/en-us/products/primary-antibodies/eif3g-antibody-epr16147-ab192601'>ab192601</a>) at 1/1000 dilution

Lane 1:

mouse Raw 264.7 cell lysate at 10 µg

Lane 2:

rat PC12 cell lysate at 10 µg

Lane 3:

mouse NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 40 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16147

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251137 is the carrier-free version of ab192601.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eIF3g also known as eukaryotic translation initiation factor 3 subunit G is a part of the eIF3 complex. It has a mass of approximately 39 kDa. This protein is expressed in various tissues throughout the body. Mechanically eIF3g participates in the process of initiation during protein synthesis by binding to the small ribosomal subunit and assisting in the recruitment of mRNA and initiator tRNA. This helps in the assembly of the functional ribosome making the translation process efficient.
Biological function summary

EIF3g plays an important role in gene expression by being part of the eIF3 complex which is important for starting translation. As a component of the eIF3 complex eIF3g influences cell growth differentiation and survival by regulating the protein synthesis rates. Researchers have identified that its presence in multiple tissues is essential for the proper functioning of cellular activities indicating its broad importance in maintaining cellular physiology.

Pathways

Experts recognize that eIF3g is integral to the mTOR signaling pathway which governs cell growth in response to nutrient availability. It also plays a significant role in the regulation of the EIF4E activity an important protein in initiating translation. eIF3g influences these pathways by acting together with other subunits of the eIF3 complex and interacting with translation-related proteins.

Disruptions in eIF3g function link to certain cancers such as breast cancer where increased expression levels of eIF3g have been associated with tumor progression. Additionally abnormalities in eIF3g may contribute to neurodegenerative diseases like Alzheimer's disease given its role in protein synthesis and cell survival. In these contexts eIF3g operates alongside proteins like eIF4E and the eIF3 complex to influence disease development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed : 17581632, PubMed : 25849773, PubMed : 27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2 : GTP : methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed : 17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed : 25849773). This subunit can bind 18S rRNA.. (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed : 18056426).
See full target information EIF3G
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34769092

2021

11,12 Epoxyeicosatrienoic Acid Rescues Deteriorated Wound Healing in Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Sommer,Heike Jakob,Caroline Reiche,Dirk Henrich,Jasmina Sterz,Johannes Frank,Ingo Marzi,Anna Lena Sander
View all publications
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Product promise

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