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Rabbit Recombinant Monoclonal EIF3G antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (AB251137), expandable thumbnail
  • Western blot - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (AB251137), expandable thumbnail
  • Immunoprecipitation - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (AB251137), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (AB251137), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWB
Human
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Rat
Expected
Expected
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Target data

Function

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). This subunit can bind 18S rRNA. (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426).

Alternative names

EIF3S4, EIF3G, Eukaryotic translation initiation factor 3 subunit G, eIF3g, Eukaryotic translation initiation factor 3 RNA-binding subunit, Eukaryotic translation initiation factor 3 subunit 4, eIF-3-delta, eIF3 p42, eIF3 p44, eIF-3 RNA-binding subunit

Recommended products

Rabbit Recombinant Monoclonal EIF3G antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR16147
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251137 is the carrier-free version of Anti-eIF3g antibody [EPR16147] ab192601.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The eIF3g also known as eukaryotic translation initiation factor 3 subunit G is a part of the eIF3 complex. It has a mass of approximately 39 kDa. This protein is expressed in various tissues throughout the body. Mechanically eIF3g participates in the process of initiation during protein synthesis by binding to the small ribosomal subunit and assisting in the recruitment of mRNA and initiator tRNA. This helps in the assembly of the functional ribosome making the translation process efficient.

Biological function summary

EIF3g plays an important role in gene expression by being part of the eIF3 complex which is important for starting translation. As a component of the eIF3 complex eIF3g influences cell growth differentiation and survival by regulating the protein synthesis rates. Researchers have identified that its presence in multiple tissues is essential for the proper functioning of cellular activities indicating its broad importance in maintaining cellular physiology.

Pathways

Experts recognize that eIF3g is integral to the mTOR signaling pathway which governs cell growth in response to nutrient availability. It also plays a significant role in the regulation of the EIF4E activity an important protein in initiating translation. eIF3g influences these pathways by acting together with other subunits of the eIF3 complex and interacting with translation-related proteins.

Associated diseases and disorders

Disruptions in eIF3g function link to certain cancers such as breast cancer where increased expression levels of eIF3g have been associated with tumor progression. Additionally abnormalities in eIF3g may contribute to neurodegenerative diseases like Alzheimer's disease given its role in protein synthesis and cell survival. In these contexts eIF3g operates alongside proteins like eIF4E and the eIF3 complex to influence disease development and progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (ab251137), expandable thumbnail

    Western blot - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (ab251137)

    This data was developed using Anti-eIF3g antibody [EPR16147] ab192601, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-eIF3g antibody [EPR16147] (Anti-eIF3g antibody [EPR16147] ab192601) at 1/1000 dilution

    Lane 1: 293T cell lysate at 20 µg

    Lane 2: MOLT-4 cell lysate at 20 µg

    Lane 3: K562 cell lysate at 20 µg

    Secondary

    All lanes: goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 36 kDa

    Observed band size: 40 kDa

  • Western blot - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (ab251137), expandable thumbnail

    Western blot - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (ab251137)

    This data was developed using Anti-eIF3g antibody [EPR16147] ab192601, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-eIF3g antibody [EPR16147] (Anti-eIF3g antibody [EPR16147] ab192601) at 1/1000 dilution

    Lane 1: mouse Raw 264.7 cell lysate at 10 µg

    Lane 2: rat PC12 cell lysate at 10 µg

    Lane 3: mouse NIH 3T3 cell lysate at 10 µg

    Secondary

    All lanes: goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 36 kDa

    Observed band size: 40 kDa

  • Immunoprecipitation - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (ab251137), expandable thumbnail

    Immunoprecipitation - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (ab251137)

    This data was developed using Anti-eIF3g antibody [EPR16147] ab192601, the same antibody clone in a different buffer formulation.

    Western blot analysis on immunoprecipitation from 1) K562 cell lysate and 2) PBS, labeling eIF3g using Anti-eIF3g antibody [EPR16147] ab192601 at 1/40 dilution and HRP-conjugated anti-rabbit IgG specific to the non-reduced form of rabbit IgG at a 1/1500 dilution.

    All lanes: Immunoprecipitation - Anti-eIF3g antibody [EPR16147] (Anti-eIF3g antibody [EPR16147] ab192601)

    Developed using the ECL technique.

    Predicted band size: 36 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (ab251137), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-eIF3g antibody [EPR16147] - BSA and Azide free (ab251137)

    This data was developed using Anti-eIF3g antibody [EPR16147] ab192601, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling eIF3g with Anti-eIF3g antibody [EPR16147] ab192601 at a 1/100 dilution ( 7.0 μg/ml). As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 was used at a 1/400 dilution. In blue DAPI staining.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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