Rabbit Polyclonal EIF3H antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EIF3H.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human EIF3H. The exact immunogen used to generate this antibody is proprietary information. Database link O15372
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Chicken | Predicted |
| Cow | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow | Dilution info - | Notes - |
Target data
Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).
Alternative names
EIF3S3, EIF3H, Eukaryotic translation initiation factor 3 subunit H, eIF3h, Eukaryotic translation initiation factor 3 subunit 3, eIF-3-gamma, eIF3 p40 subunit
Recommended products
Rabbit Polyclonal EIF3H antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EIF3H.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human EIF3H. The exact immunogen used to generate this antibody is proprietary information. Database link O15372
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EIF3H also known as eukaryotic translation initiation factor 3 subunit H is a component of the eIF3 complex which plays a substantial role in translation initiation. The protein possesses a molecular mass of approximately 42 kDa. EIF3H is broadly expressed in various tissues and cells including high expression levels in proliferating cells. The protein is part of the larger eIF3 complex which consists of 13 subunits contributing to the formation of the pre-initiation complex essential for mRNA translation.
- Biological function summary
EIF3H facilitates the recruitment of mRNA to the ribosome by stabilizing the interaction between mRNA and the 40S ribosomal subunit. It functions within the eIF3 complex to assist in ribosomal scanning and the start codon selection. As part of this complex EIF3H helps govern the efficiency of translation initiation impacting protein synthesis rates. These activities are important for cell growth and protein expression regulation underlying the complex's importance in cellular processes.
- Pathways
EIF3H plays a role in the mTOR signaling pathway and the MAPK/ERK pathway both extensively involved in regulating cell growth and proliferation. Through these pathways EIF3H interacts with proteins such as eIF4G and ribosomal protein S6 which help coordinate the translation machinery's function and cellular metabolic control. These pathways highlight EIF3H's role in translational control affecting overall cellular function.
- Associated diseases and disorders
EIF3H is implicated in cancer particularly in types characterized by unchecked cellular proliferation such as lung and breast cancer. EIF3H's dysregulated expression or activity can influence oncogenic pathways leading to malignancies. Additionally EIF3H interacts with proteins like c-Myc a well-known oncogene through its involvement in cancer pathways. These associations highlight EIF3H as a potential target for therapeutic intervention in cancer treatments.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-EIF3H antibody (ab228536)
All lanes: Western blot - Anti-EIF3H antibody (ab228536) at 1/1000 dilution
Lane 1: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Lane 2: NCI-H1229 (human lung carcinoma cell line) whole cell lysate at 30 µg
Lane 3: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com