AB251743

Anti-EIF3H antibody

Name: Anti-EIF3H antibody (ab251743) | Abcam
Brand: Abcam Limited
SKU: AB251743
Price: 655 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal EIF3H antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EIF3H aa 250 to C-terminus.

View Alternative Names

EIF3S3, EIF3H, Eukaryotic translation initiation factor 3 subunit H, eIF3h, Eukaryotic translation initiation factor 3 subunit 3, eIF-3-gamma, eIF3 p40 subunit

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3H antibody (AB251743)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3H antibody (AB251743)

Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis in human testis tissue labeling EIF3H with ab251743 at 1/1000 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3H antibody (AB251743)

Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis in human duodenum tissue labeling EIF3H with ab251743 at 1/1000 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-EIF3H antibody (AB251743)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EIF3H antibody (AB251743)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for EIF3H (green) using ab251743 at 4 µg/ml in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3H antibody (AB251743)

Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis in human cerebellum tissue labeling EIF3H with ab251743 at 1/1000 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3H antibody (AB251743)

Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis in human cervix tissue labeling EIF3H with ab251743 at 1/1000 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6.0 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-EIF3H antibody (AB251743)

All lanes:

Western blot - Anti-EIF3H antibody (ab251743) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) whole cell lysate

Lane 3:

Human plasma (IgG/HSA depleted)

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 40 kDa

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Western blot - Anti-EIF3H antibody (AB251743)

All lanes:

Western blot - Anti-EIF3H antibody (ab251743) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lane 2:

NBT-II (rat Wistar bladder tumor cell line) whole cell lysate

Predicted band size: 40 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human EIF3H aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15372

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF3H also known as eukaryotic translation initiation factor 3 subunit H is a component of the eIF3 complex which plays a substantial role in translation initiation. The protein possesses a molecular mass of approximately 42 kDa. EIF3H is broadly expressed in various tissues and cells including high expression levels in proliferating cells. The protein is part of the larger eIF3 complex which consists of 13 subunits contributing to the formation of the pre-initiation complex essential for mRNA translation.

Biological function summary

EIF3H facilitates the recruitment of mRNA to the ribosome by stabilizing the interaction between mRNA and the 40S ribosomal subunit. It functions within the eIF3 complex to assist in ribosomal scanning and the start codon selection. As part of this complex EIF3H helps govern the efficiency of translation initiation impacting protein synthesis rates. These activities are important for cell growth and protein expression regulation underlying the complex's importance in cellular processes.

Pathways

EIF3H plays a role in the mTOR signaling pathway and the MAPK/ERK pathway both extensively involved in regulating cell growth and proliferation. Through these pathways EIF3H interacts with proteins such as eIF4G and ribosomal protein S6 which help coordinate the translation machinery's function and cellular metabolic control. These pathways highlight EIF3H's role in translational control affecting overall cellular function.

EIF3H is implicated in cancer particularly in types characterized by unchecked cellular proliferation such as lung and breast cancer. EIF3H's dysregulated expression or activity can influence oncogenic pathways leading to malignancies. Additionally EIF3H interacts with proteins like c-Myc a well-known oncogene through its involvement in cancer pathways. These associations highlight EIF3H as a potential target for therapeutic intervention in cancer treatments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed : 17581632, PubMed : 25849773, PubMed : 27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2 : GTP : methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed : 17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed : 25849773).

See full target information EIF3H

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 25:435 PubMed40069645

2025

CGREF1 facilitates the cell proliferation, migration and invasion of hepatocellular carcinoma cells via regulation of EIF3H/ Wnt/β-Catenin signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dongkai Gao,Zumo Zhou,Lin Chen,Jun Zheng,Jinna Yang

Journal of experimental & clinical cancer research 39:175 PubMed32867821

2020

EIF3H promotes aggressiveness of esophageal squamous cell carcinoma by modulating Snail stability.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobin Guo,Rui Zhu,Aiping Luo,Honghong Zhou,Fang Ding,Hongxin Yang,Zhihua Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EIF3H antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3H antibody (AB251743)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3H antibody (AB251743)

Immunocytochemistry/ Immunofluorescence - Anti-EIF3H antibody (AB251743)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3H antibody (AB251743)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3H antibody (AB251743)

Western blot - Anti-EIF3H antibody (AB251743)

Western blot - Anti-EIF3H antibody (AB251743)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise