Rabbit Polyclonal EIF3K antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human EIF3K aa 150 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human EIF3K aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UBQ5
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla | Dilution info - | Notes - |
Target data
Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).
Alternative names
EIF3S12, ARG134, HSPC029, MSTP001, PTD001, EIF3K, Eukaryotic translation initiation factor 3 subunit K, eIF3k, Eukaryotic translation initiation factor 3 subunit 12, Muscle-specific gene M9 protein, PLAC-24, eIF-3 p25, eIF-3 p28
Recommended products
Rabbit Polyclonal EIF3K antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human EIF3K aa 150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human EIF3K aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UBQ5
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EIF3K also known as eukaryotic translation initiation factor 3 subunit K is a protein subunit of the eIF3 complex. This protein has a mass of approximately 30 kilodaltons and it is expressed in many tissues across the body. The mechanical role of eIF3K is primarily in the assembly and functioning of the translation initiation complex which positions ribosomes at the start codon of messenger RNA (mRNA) strands to begin protein synthesis.
- Biological function summary
EIF3K is involved in the regulation of the eukaryotic translation initiation factor 3 complex which is essential for initiating translation by serving as a scaffold for other eIF3 subunits. This complex stabilizes the formation of the pre-initiation complex necessary for ribosome binding to mRNA. Through its involvement in protein synthesis eIF3K influences cell growth proliferation and response to various cellular signals. It is involved in processes such as cell cycle control and apoptotic regulation as it aids the translation of specific mRNAs coding for key regulatory proteins.
- Pathways
EIF3K participates in mTOR and Wnt signaling pathways. These pathways are critical in controlling cell growth proliferation and differentiation. Through mTOR signaling eIF3K interacts with proteins like ribosomal protein S6 kinase beta-1 (S6K1) influencing cell metabolism and growth. In the Wnt pathway eIF3K contributes to the translation of proteins that regulate cell fate and stem cell renewal. Interactions with these pathways illustrate the importance of eIF3K in maintaining cellular homeostasis.
- Associated diseases and disorders
Alterations in eIF3K function have links to cancer and neurodegenerative diseases. In cancer dysregulated eIF3K expression can lead to unregulated cell growth and tumorigenesis due to its role in protein synthesis and cell proliferation. eIF3K connects to oncogenic proteins such as c-Myc which stimulate cancer progression. In neurodegenerative diseases eIF3K may contribute to cell death through its interactions with apoptotic pathways and impaired cellular stress responses highlighting its potential as a target for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-eIF3K antibody (ab85968)
All lanes: Western blot - Anti-eIF3K antibody (ab85968) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: 293T whole cell lysate at 50 µg
Predicted band size: 25 kDa
Exposure time: 10s
Immunoprecipitation - Anti-eIF3K antibody (ab85968)
1 mg of HeLa whole cell lysate was immunoprecipitated using 3µg ab85968. Subsequently, 20% of IP was loaded per lane and probed with ab85968 at 1µg/ml (lane 1) or with a control IgG (lane 2).
Detection: chemiluminescence with exposure time of 3 seconds.All lanes: Immunoprecipitation - Anti-eIF3K antibody (ab85968)
Predicted band size: 25 kDa
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