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AB154527

Anti-eIF3l antibody

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(1 Publication)

Rabbit Polyclonal EIF3L antibody. Suitable for WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EIF3L aa 300 to C-terminus.

View Alternative Names

EIF3EIP, EIF3S6IP, HSPC021, HSPC025, MSTP005, EIF3L, Eukaryotic translation initiation factor 3 subunit L, eIF3l, Eukaryotic translation initiation factor 3 subunit 6-interacting protein, Eukaryotic translation initiation factor 3 subunit E-interacting protein

1 Images
Western blot - Anti-eIF3l antibody (AB154527)
  • WB

Unknown

Western blot - Anti-eIF3l antibody (AB154527)

7.5% SDS PAGE

All lanes:

Western blot - Anti-eIF3l antibody (ab154527) at 1/1000 dilution

Lane 1:

Mouse brown adipose whole cell lysate at 50 µg

Lane 2:

Mouse white adipose whole cell lysate at 50 µg

Predicted band size: 66 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human EIF3L aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y262

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eukaryotic translation initiation factor 3 subunit L (eIF3l) also known as eIF-3 eta plays an essential role in the initiation of protein synthesis by facilitating the assembly of the pre-initiation complex on the mRNA. This protein with a mass of approximately 44 kDa is ubiquitously expressed across various tissues indicating its importance in overall cellular processes. As a component of the eIF3 complex it helps in regulating the translation initiation step which is a critical control point in protein synthesis.
Biological function summary

EIF3l interacts with other components of the eIF3 complex to ensure the proper function of the translation machinery. This subunit as part of the eIF3 complex helps in the recruitment of the 40S ribosomal subunit to the 5' mRNA cap structure which is a necessary step for the initiation of translation. The eIF3 complex consists of multiple subunits and eIF3l specifically plays a role in the stability and function of this complex contributing to the efficiency and fidelity of protein biosynthesis.

Pathways

EIF3l participates heavily in the translation initiation pathway which is a major step in gene expression regulation. This process influences many other pathways involving cell growth and proliferation such as the mTOR signaling pathway. In this context eIF3l interacts with other elements of the translation machinery such as the protein eIF4F which together impact processes like cap-dependent translation and cellular response to nutrient availability.

EIF3l has connections to cancer and neurodegenerative diseases indicating its significance in both proliferation-related and degenerative pathways. Abnormal expression or malfunction of eIF3l can potentially lead to disorders such as specific types of cancer due to its role in uncontrolled cell growth and protein synthesis. In addition eIF3l's interaction with key proteins like mTOR connects it to pathways involved in oncogenesis and cancer cell survival strategies further highlighting its clinical importance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed : 17581632, PubMed : 25849773, PubMed : 27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2 : GTP : methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed : 17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed : 25849773).. (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed : 18056426).
See full target information EIF3L
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine : PubMed33939305

2021

cIAP1/2 are involved in the radiosensitizing effect of birinapant on NSCLC cell line in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Sun,Yanan Du,Ming Yao,Qin Wang,Kaihua Ji,Liqing Du,Chang Xu,Ningning He,Jinhan Wang,Manman Zhang,Yang Liu,Yan Wang,Kaixue Wen,Qiang Liu
View all publications
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Product promise

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