AB241367

Anti-eIF3l antibody

Name: Anti-eIF3l antibody (ab241367) | Abcam
Brand: Abcam Limited
SKU: AB241367
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal EIF3L antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EIF3L aa 1-50.

View Alternative Names

EIF3EIP, EIF3S6IP, HSPC021, HSPC025, MSTP005, EIF3L, Eukaryotic translation initiation factor 3 subunit L, eIF3l, Eukaryotic translation initiation factor 3 subunit 6-interacting protein, Eukaryotic translation initiation factor 3 subunit E-interacting protein

2 Images

Supplier Data

Immunoprecipitation - Anti-eIF3l antibody (AB241367)

eIF3l was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241367 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241367 at 0.4 μg/ml.

Lane 1 : ab241367 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

Lysate prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-eIF3l antibody (ab241367)

Predicted band size: 66 kDa

false

Supplier Data

Western blot - Anti-eIF3l antibody (AB241367)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-eIF3l antibody (ab241367) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 66 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human EIF3L aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9Y262

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab241367 was affinity purified using an epitope specific to eIF3l immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eukaryotic translation initiation factor 3 subunit L (eIF3l) also known as eIF-3 eta plays an essential role in the initiation of protein synthesis by facilitating the assembly of the pre-initiation complex on the mRNA. This protein with a mass of approximately 44 kDa is ubiquitously expressed across various tissues indicating its importance in overall cellular processes. As a component of the eIF3 complex it helps in regulating the translation initiation step which is a critical control point in protein synthesis.

Biological function summary

EIF3l interacts with other components of the eIF3 complex to ensure the proper function of the translation machinery. This subunit as part of the eIF3 complex helps in the recruitment of the 40S ribosomal subunit to the 5' mRNA cap structure which is a necessary step for the initiation of translation. The eIF3 complex consists of multiple subunits and eIF3l specifically plays a role in the stability and function of this complex contributing to the efficiency and fidelity of protein biosynthesis.

Pathways

EIF3l participates heavily in the translation initiation pathway which is a major step in gene expression regulation. This process influences many other pathways involving cell growth and proliferation such as the mTOR signaling pathway. In this context eIF3l interacts with other elements of the translation machinery such as the protein eIF4F which together impact processes like cap-dependent translation and cellular response to nutrient availability.

EIF3l has connections to cancer and neurodegenerative diseases indicating its significance in both proliferation-related and degenerative pathways. Abnormal expression or malfunction of eIF3l can potentially lead to disorders such as specific types of cancer due to its role in uncontrolled cell growth and protein synthesis. In addition eIF3l's interaction with key proteins like mTOR connects it to pathways involved in oncogenesis and cancer cell survival strategies further highlighting its clinical importance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed : 17581632, PubMed : 25849773, PubMed : 27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2 : GTP : methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed : 17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed : 25849773).. (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed : 18056426).

See full target information EIF3L

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 7:392 PubMed34907164

2021

CRISPR/Cas9 genome-wide screening identifies LUC7L2 that promotes radioresistance via autophagy in nasopharyngeal carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Shen,Chao Li,Fang Chen,Liangfang Shen,Zhanzhan Li,Na Li

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-eIF3l antibody

Immunoprecipitation - Anti-eIF3l antibody (AB241367)

Western blot - Anti-eIF3l antibody (AB241367)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise