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AB239071

Anti-EIF3S1/EIF3J antibody

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(1 Publication)

Rabbit Polyclonal EIF3S1/EIF3J antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EIF3J aa 1-150.

View Alternative Names

EIF3S1, PRO0391, EIF3J, Eukaryotic translation initiation factor 3 subunit J, eIF3j, Eukaryotic translation initiation factor 3 subunit 1, eIF-3-alpha, eIF3 p35

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3S1/EIF3J antibody (AB239071)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3S1/EIF3J antibody (AB239071)

Paraffin-embedded human kidney tissue stained for EIF3S1/EIF3J with ab239071 at a 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human EIF3J aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O75822

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF3S1 also known as EIF3J is a subunit of the eukaryotic initiation factor 3 (eIF3) complex. It has a molecular mass of approximately 29 kDa. This protein is integral to the assembly of the eIF3 complex which plays an important role in the initiation phase of translation. EIF3J is expressed in various tissues including those with high rates of protein synthesis such as muscle and liver.
Biological function summary

The eIF3J protein functions as part of the eIF3 complex to facilitate the formation of the 43S pre-initiation complex. It assists in stabilizing the interaction between the small ribosomal subunit and mRNA enhancing the accuracy and efficiency of translation initiation. The eIF3 complex is composed of 13 subunits and EIF3J contributes to the structural integrity and functionality of this complex making it essential for proper translation initiation.

Pathways

EIF3J participates in the mTOR signaling pathway and the MAPK signaling pathway. These pathways regulate cell growth proliferation and survival. In the mTOR pathway the eIF3 complex including EIF3J associates with proteins like mTORC1 to influence protein synthesis and cell growth. In the MAPK pathway EIF3J helps control translation in response to extracellular stimuli through its involvement in the eIF3 complex.

EIF3J is linked to cancer and neurodegenerative diseases. Altered expression or dysfunction of the eIF3 complex involving EIF3J can lead to unregulated protein synthesis contributing to tumorigenesis. Additionally anomalies in translation can impact proteins associated with neurodegenerative conditions like Alzheimer's disease where improper protein synthesis regulation can exacerbate disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed : 25849773, PubMed : 27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2 : GTP : methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed : 25849773).
See full target information EIF3J
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

ASN neuro 13:17590914211044359 PubMed34618621

2021

Role of EphrinA3 in HIV-1 Neuropathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chitra Mohinder Singh Singal,Paritosh Jaiswal,Anuradha Mehta,Kanza Saleem,Pankaj Seth
View all publications
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Product promise

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