Anti-eIF4A1 antibody (ab31217) is a rabbit polyclonal antibody detecting eIF4A1 in Western Blot, IP, IHC-P, ICC/IF. Suitable for African green monkey, Cow, Human, Mouse, Rat.
- Over 50 publications
- Trusted since 2006
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- PloS one 18:e02920802023The Helix-Loop-Helix motif of human EIF3A regulates translation of proliferative cellular mRNAs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarina P Volegova,Cynthia Hermosillo,Jamie H D CatePubMed 37768948
- Cells 12:2023A Practical and Analytical Comparative Study of Gel-Based Top-Down and Gel-Free Bottom-Up Proteomics Including Unbiased Proteoform Detection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuriye Ercan,Ulrike Resch,Felicia Hsu,Goran Mitulovic,Andrea Bileck,Christopher Gerner,Jae-Won Yang,Margarethe Geiger,Ingrid Miller,Maria ZellnerPubMed 36899884
- Nucleic acids research 51:1859-18792023eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTobias Schmidt,Adrianna Dabrowska,Joseph A Waldron,Kelly Hodge,Grigorios Koulouras,Mads Gabrielsen,June Munro,David C Tack,Gemma Harris,Ewan McGhee,David Scott,Leo M Carlin,Danny Huang,John Le Quesne,Sara Zanivan,Ania Wilczynska,Martin BushellPubMed 36727461
- Nucleic acids research 51:1326-13522023Identification of RNA helicases with unwinding activity on angiogenin-processed tRNAs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAleksej Drino,Lisa König,Charlotte Capitanchik,Nasim Sanadgol,Eva Janisiw,Tom Rappol,Elisa Vilardo,Matthias R SchaeferPubMed 36718960
- International journal of molecular sciences 24:2023eIF4A1 Is a Prognostic Marker and Actionable Target in Human Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara M Steinmann,Anabel Sánchez-Martín,Elisabeth Tanzer,Antonio Cigliano,Giovanni M Pes,Maria M Simile,Laurent Desaubry,Jose J G Marin,Matthias Evert,Diego F CalvisiPubMed 36768380
- Cell death & disease 13:9332022Splicing factor SRSF3 represses translation of p21 mRNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeeho Kim,Ra Young Park,Younghoon Kee,Sunjoo Jeong,Takbum OhnPubMed 36344491
- Molecular medicine (Cambridge, Mass.) 28:1012022Novel eIF4A1 inhibitors with anti-tumor activity in lymphoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesForum Kayastha,Noah B Herrington,Bandish Kapadia,Anirban Roychowdhury,Nahid Nanaji,Glen E Kellogg,Ronald B GartenhausPubMed 36058921
- Molecular cell 82:2401-2414.e92022Localization of a TORC1-eIF4F translation complex during CD8 T cell activation drives divergent cell fate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSwantje Liedmann,Xueyan Liu,Clifford S Guy,Jeremy Chase Crawford,Diego A Rodriguez,Duygu Kuzuoğlu-Öztürk,Ao Guo,Katherine C Verbist,Jamshid Temirov,Mark J Chen,Davide Ruggero,Hui Zhang,Paul G Thomas,Douglas R GreenPubMed 35597236
- Journal of extracellular vesicles 11:e122212022Extracellular vesicles rich in HAX1 promote angiogenesis by modulating ITGB6 translation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo You,Si Pan,Miao Gu,Kaiwen Zhang,Tian Xia,Siyu Zhang,Wenhui Chen,Haijing Xie,Yue Fan,Hui Yao,Tianyi Cheng,Panpan Zhang,Dong Liu,Yiwen YouPubMed 35524442
- Frontiers in immunology 13:8747922022Identification and Verification of mG Modification Patterns and Characterization of Tumor Microenvironment Infiltration Multi-Omics Analysis in Clear Cell Renal Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Dong,Di Gu,Jiazi Shi,Yewei Bao,Zhibin Fu,Yu Fang,Le Qu,Wentong Zhu,Aimin Jiang,Linhui WangPubMed 35592316
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested |
| Mouse | Expected | Tested | Expected | Expected |
| Rat | Predicted | Predicted | Expected | Predicted |
| African green monkey | Predicted | Predicted | Expected | Predicted |
| Cow | Predicted | Predicted | Expected | Predicted |
| Monkey | Predicted | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Monkey, African green monkey, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Monkey, African green monkey, Cow | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, African green monkey, Human, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Monkey, African green monkey, Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome (PubMed:20156963). In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. As a result, promotes cell proliferation and growth (PubMed:20156963).
Alternative names
DDX2A, EIF4A, EIF4A1, Eukaryotic initiation factor 4A-I, eIF-4A-I, eIF4A-I, ATP-dependent RNA helicase eIF4A-1
Recommended products
Anti-eIF4A1 antibody (ab31217) is a rabbit polyclonal antibody detecting eIF4A1 in Western Blot, IP, IHC-P, ICC/IF. Suitable for African green monkey, Cow, Human, Mouse, Rat.
- Over 50 publications
- Trusted since 2006
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Replenishment batches of our polyclonal antibody, ab31217 are tested in WB. Previous batches were additionally validated in ICC/IF, IHC-P and IP. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab185946.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-eIF4A1 antibody (ab31217) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in African green monkey, Cow, Human, Mouse, Rat samples.
What is the molecular weight of eIF4A1?
Anti-eIF4A1 (ab31217) specifically detects a band for eIF4A1 (UniProt: P60842) at a molecular weight of 47kDa.
Trusted by the scientific community
Anti-eIF4A1 (ab31217) was first used in a scientific publication in 2006 and has been cited over 50 times in peer-reviewed journals.
Reviewed by scientists
Anti-eIF4A1 (ab31217) has over 5 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The eukaryotic initiation factor 4A1 also known as eIF4A1 is an essential RNA helicase part of the DEAD-box family. It unwinds secondary structures in the 5' untranslated region of mRNA facilitating ribosome binding during translation initiation. This protein has a molecular mass of approximately 46 kDa. Expressed ubiquitously in eukaryotic cells eIF4A1 plays a central role in regulating protein synthesis by enhancing the accessibility of mRNA templates to the ribosome.
- Biological function summary
The eIF4A1 protein functions as a component of the eIF4F complex which includes eIF4E and eIF4G. This complex modulates the initiation of mRNA translation a critical step in gene expression. By associating with other initiation factors eIF4A1 helps orchestrate the recruitment of the ribosome to capped mRNA. Its activity influences cell growth proliferation and response to environmental signals.
- Pathways
EIF4A1 involves in the regulation of the mTOR signaling pathway which controls cell growth based on nutrient availability. It also participates in the integrated stress response pathway linking stress signals to translational control. eIF4A1 interacts with other proteins such as eIF4E and eIF4G through its function within these pathways highlighting its role in translation regulation.
- Associated diseases and disorders
EIF4A1 has been implicated in cancer and neurodegenerative diseases. Aberrant regulation of eIF4A1 expression and activity contributes to oncogenesis by promoting uncontrolled protein synthesis and cell proliferation. In neurodegenerative disorders altered eIF4A1 activity disrupts normal neuronal function and viability. Connections to proteins like eIF4E highlight its impact on disease progression emphasizing the importance of understanding eIF4A1's functional mechanisms in these contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Western blot - Anti-eIF4A1 antibody (ab31217)
All lanes: Western blot - Anti-eIF4A1 antibody (ab31217) at 1 µg/mL
Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: Liver (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 4: Western blot - Mouse pancreas tissue lysate - total protein (Mouse pancreas tissue lysate - total protein ab29363) at 10 µg
Lane 5: Testis (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 6: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 47 kDa
Western blot - Anti-eIF4A1 antibody (ab31217)
All lanes: Western blot - Anti-eIF4A1 antibody (ab31217) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 2: Western blot - Jurkat whole cell lysate (Jurkat whole cell lysate ab7899) at 20 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 47 kDa
Immunocytochemistry/ Immunofluorescence - Anti-eIF4A1 antibody (ab31217)
ICC/IF image of ab31217 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab31217, 1μg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used to quench autofluorescence. 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Immunoprecipitation - Anti-eIF4A1 antibody (ab31217)
eIF4A1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to eIF4A1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31217.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) ab99697).
Band: 47kDa: eIF4A1All lanes: Immunoprecipitation - Anti-eIF4A1 antibody (ab31217)
Predicted band size: 46 kDa
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