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Rabbit Polyclonal eIF4A1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat, African green monkey, Cow samples. Cited in 59 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IF
Human
Tested
Tested
Expected
Tested
Mouse
Expected
Tested
Expected
Expected
Rat
Predicted
Predicted
Expected
Predicted
African green monkey
Predicted
Predicted
Expected
Predicted
Cow
Predicted
Predicted
Expected
Predicted
Monkey
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Monkey, African green monkey, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1 µg/mL

Notes

-

Species

Human

Dilution info

1 µg/mL

Notes

-

Predicted
Predicted

Species

Rat, Monkey, African green monkey, Cow

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat, African green monkey, Human, Cow

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Monkey, African green monkey, Cow

Dilution info

-

Notes

-

Target data

Function

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Alternative names

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Rabbit Polyclonal eIF4A1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat, African green monkey, Cow samples. Cited in 59 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Purification technique

Affinity purification Immunogen

Specificity

Replenishment batches of our polyclonal antibody, ab31217 are tested in WB. Previous batches were additionally validated in ICC/IF, IHC-P and IP. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab185946.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological summary

The eIF4A1 protein functions as a component of the eIF4F complex which includes eIF4E and eIF4G. This complex modulates the initiation of mRNA translation a critical step in gene expression. By associating with other initiation factors eIF4A1 helps orchestrate the recruitment of the ribosome to capped mRNA. Its activity influences cell growth proliferation and response to environmental signals.

Mechanical summary

The eukaryotic initiation factor 4A1 also known as eIF4A1 is an essential RNA helicase part of the DEAD-box family. It unwinds secondary structures in the 5' untranslated region of mRNA facilitating ribosome binding during translation initiation. This protein has a molecular mass of approximately 46 kDa. Expressed ubiquitously in eukaryotic cells eIF4A1 plays a central role in regulating protein synthesis by enhancing the accessibility of mRNA templates to the ribosome.

Pathway

EIF4A1 involves in the regulation of the mTOR signaling pathway which controls cell growth based on nutrient availability. It also participates in the integrated stress response pathway linking stress signals to translational control. eIF4A1 interacts with other proteins such as eIF4E and eIF4G through its function within these pathways highlighting its role in translation regulation.

Disease

EIF4A1 has been implicated in cancer and neurodegenerative diseases. Aberrant regulation of eIF4A1 expression and activity contributes to oncogenesis by promoting uncontrolled protein synthesis and cell proliferation. In neurodegenerative disorders altered eIF4A1 activity disrupts normal neuronal function and viability. Connections to proteins like eIF4E highlight its impact on disease progression emphasizing the importance of understanding eIF4A1's functional mechanisms in these contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-eIF4A1 antibody (ab31217), expandable thumbnail

    Western blot - Anti-eIF4A1 antibody (ab31217)

    All lanes: Western blot - Anti-eIF4A1 antibody (AB31217) at 1 µg/mL

    Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

    Lane 2: Western blot - Jurkat whole cell lysate (AB7899) at 20 µg

    Lane 3: Western blot - A-431 whole cell lysate (AB7909) at 20 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 46 kDa

    Observed band size: 47 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4A1 antibody (ab31217), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-eIF4A1 antibody (ab31217)

    ICC/IF image of ab31217 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab31217, 1μg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used to quench autofluorescence. 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • Immunoprecipitation - Anti-eIF4A1 antibody (ab31217), expandable thumbnail

    Immunoprecipitation - Anti-eIF4A1 antibody (ab31217)

    eIF4A1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to eIF4A1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31217.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 47kDa: eIF4A1

    All lanes: Immunoprecipitation - Anti-eIF4A1 antibody (AB31217)

    Predicted band size: 46 kDa

  • Western blot - Anti-eIF4A1 antibody (ab31217), expandable thumbnail

    Western blot - Anti-eIF4A1 antibody (ab31217)

    All lanes: Western blot - Anti-eIF4A1 antibody (AB31217) at 1 µg/mL

    Lane 1: Western blot - NIH/3T3 whole cell lysate (AB7179) at 10 µg

    Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Lane 3: Liver (Mouse) Tissue Lysate - normal tissue at 10 µg

    Lane 4: Western blot - Mouse pancreas tissue lysate - total protein (AB29363) at 10 µg

    Lane 5: Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

    Lane 6: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 46 kDa

    Observed band size: 47 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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