AB185946

Anti-eIF4A1 antibody [EPR14506]

Name: Anti-eIF4A1 antibody [EPR14506] (ab185946) | Abcam
Brand: Abcam Limited
SKU: AB185946
Price: 580 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal eIF4A1 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

DDX2A, EIF4A, EIF4A1, Eukaryotic initiation factor 4A-I, eIF-4A-I, eIF4A-I, ATP-dependent RNA helicase eIF4A-1

3 Images

Supplier Data

Immunoprecipitation - Anti-eIF4A1 antibody [EPR14506] (AB185946)

Western blot analysis on immunoprecipitation from 1) 293 cell lysate and 2) PBS, labeling eIF4A1 using ab185946 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-eIF4A1 antibody [EPR14506] (ab185946)

Predicted band size: 46 kDa

Observed band size: 46 kDa

false

Supplier Data

Western blot - Anti-eIF4A1 antibody [EPR14506] (AB185946)

All lanes:

Western blot - Anti-eIF4A1 antibody [EPR14506] (ab185946) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 46 kDa

Observed band size: 46 kDa

false

Supplier Data

Western blot - Anti-eIF4A1 antibody [EPR14506] (AB185946)

All lanes:

Western blot - Anti-eIF4A1 antibody [EPR14506] (ab185946) at 1/1000 dilution

Lane 1:

rat C6 cell lysate at 10 µg

Lane 2:

mouse Raw264.7 cell lysate at 10 µg

Lane 3:

rat PC12 cell lysate at 10 µg

Lane 4:

mouse NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 46 kDa

Observed band size: 46 kDa

false

Carrier free

Anti-eIF4A1 antibody [EPR14506] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14506

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 93% homology with eIF4A2. Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eukaryotic initiation factor 4A1 also known as eIF4A1 is an essential RNA helicase part of the DEAD-box family. It unwinds secondary structures in the 5' untranslated region of mRNA facilitating ribosome binding during translation initiation. This protein has a molecular mass of approximately 46 kDa. Expressed ubiquitously in eukaryotic cells eIF4A1 plays a central role in regulating protein synthesis by enhancing the accessibility of mRNA templates to the ribosome.

Biological function summary

The eIF4A1 protein functions as a component of the eIF4F complex which includes eIF4E and eIF4G. This complex modulates the initiation of mRNA translation a critical step in gene expression. By associating with other initiation factors eIF4A1 helps orchestrate the recruitment of the ribosome to capped mRNA. Its activity influences cell growth proliferation and response to environmental signals.

Pathways

EIF4A1 involves in the regulation of the mTOR signaling pathway which controls cell growth based on nutrient availability. It also participates in the integrated stress response pathway linking stress signals to translational control. eIF4A1 interacts with other proteins such as eIF4E and eIF4G through its function within these pathways highlighting its role in translation regulation.

EIF4A1 has been implicated in cancer and neurodegenerative diseases. Aberrant regulation of eIF4A1 expression and activity contributes to oncogenesis by promoting uncontrolled protein synthesis and cell proliferation. In neurodegenerative disorders altered eIF4A1 activity disrupts normal neuronal function and viability. Connections to proteins like eIF4E highlight its impact on disease progression emphasizing the importance of understanding eIF4A1's functional mechanisms in these contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome (PubMed : 20156963). In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. As a result, promotes cell proliferation and growth (PubMed : 20156963).

See full target information EIF4A1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 15:e70270 PubMed40088428

2025

Targeting YBX1-m5C mediates RNF115 mRNA circularisation and translation to enhance vulnerability of ferroptosis in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ouwen Li,Ke An,Han Wang,Xianbin Li,Yueqin Wang,Lan Huang,Yue Du,Nuo Qin,Jiasheng Dong,Jingyao Wei,Ranran Sun,Yong Shi,Yanjia Guo,Xiangyi Sun,Ying Yang,Yun-Gui Yang,Quancheng Kan,Xin Tian

Nature communications 15:7249 PubMed39179537

2024

Macrophage ILF3 promotes abdominal aortic aneurysm by inducing inflammatory imbalance in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao-Yang Wang,Jie Cheng,Ying Wang,Hai-Tao Yuan,Shao-Jie Bi,Shuang-Xi Wang,Ya-Min Hou,Xu Zhang,Bo-Han Xu,Ze-Ying Wang,Yun Zhang,Wen-Jian Jiang,Yu-Guo Chen,Ming-Xiang Zhang

Bioengineered 13:10111-10122 PubMed35435117

2022

Astragaloside IV exhibits anti-tumor function in gastric cancer via targeting circRNA dihydrolipoamide S-succinyltransferase (circDLST)/miR-489-3p/ eukaryotic translation initiation factor 4A1(EIF4A1) pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fagen Li,Ke Cao,Maoyun Wang,Yi Liu,Yin Zhang

Nature communications 10:2682 PubMed31213602

2019

CAPRI enables comparison of evolutionarily conserved RNA interacting regions.

Applications

Unspecified application

Species

Unspecified reactive species

Amol Panhale,Florian M Richter,Fidel Ramírez,Maria Shvedunova,Thomas Manke,Gerhard Mittler,Asifa Akhtar

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-eIF4A1 antibody [EPR14506]

Immunoprecipitation - Anti-eIF4A1 antibody [EPR14506] (AB185946)

Western blot - Anti-eIF4A1 antibody [EPR14506] (AB185946)

Western blot - Anti-eIF4A1 antibody [EPR14506] (AB185946)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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