AB194471

Anti-eIF4A2 antibody

Name: Anti-eIF4A2 antibody (ab194471) | Abcam
Brand: Abcam Limited
SKU: AB194471
Price: 535 USD
Availability: InStock
Rating: 5 (3 reviews)

(3 Reviews)

(2 Publications)

Mouse Polyclonal eIF4A2 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EIF4A2 aa 1-100.

View Alternative Names

DDX2B, EIF4F, EIF4A2, Eukaryotic initiation factor 4A-II, eIF-4A-II, eIF4A-II, ATP-dependent RNA helicase eIF4A-2

2 Images

Supplier Data

Western blot - Anti-eIF4A2 antibody (AB194471)

All lanes:

Western blot - Anti-eIF4A2 antibody (ab194471) at 1/500 dilution

All lanes:

Jurkat cell lysate at 50 µg

Predicted band size: 46 kDa

true

Supplier Data

Western blot - Anti-eIF4A2 antibody (AB194471)

Predicted MWt of immunogen : 37.11 kDa.

All lanes:

Western blot - Anti-eIF4A2 antibody (ab194471) at 1/1000 dilution

All lanes:

Recombinant Human eIF4A2 protein (immunogen) at 0.2 µg

Predicted band size: 37 kDa,46 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human EIF4A2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q14240

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The eIF4A2 also known as DDX2B functions as an RNA helicase with an ATP-dependent mechanism. It unwinds RNA secondary structures in the 5'UTR of mRNAs to facilitate ribosome binding. eIF4A2 belongs to the DEAD-box family of proteins and it has a molecular mass of approximately 46 kDa. It is ubiquitously expressed in various tissues indicating its broad role in cellular processes.

Biological function summary

EIF4A2 is integral to the initiation of protein synthesis. It acts as a core member of the eIF4F complex along with the cap-binding protein eIF4E and scaffold protein eIF4G. This complex enables the recruitment of ribosomes to mRNA an important step in translation initiation. eIF4A2 helps regulate the translation of specific mRNAs that are important for cell growth and proliferation affecting how cells respond to environmental stimuli.

Pathways

EIF4A2 plays a significant role in the mTOR and MAPK signaling pathways. In the mTOR pathway eIF4A2 controls the translation of mRNAs that drive cell growth and metabolism. It also interacts with other proteins such as mTORC1 and S6K which modulate these processes. Its involvement in the MAPK pathway links it with cell survival and stress responses emphasizing its interaction with proteins like MEK1/2 and ERK1/2 that further propagate these signals.

EIF4A2 has implications in cancer and neurodegenerative diseases. eIF4A2's overexpression has been associated with various cancers whereby it encourages oncogenic transformation through its role in synthesis of growth-related proteins. Moreover the helicase has links to Alzheimer's disease where it may influence disease progression by modulating synaptic plasticity proteins such as APP and tau important for neuronal function. The regulation by eIF4A2 of these proteins highlights its potential as a therapeutic target.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

See full target information EIF4A2

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Science advances 8:eabm0478 PubMed35353581

2022

eIF4A2 targets developmental potency and histone H3.3 transcripts for translational control of stem cell pluripotency.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Li,Jihong Yang,Xin Huang,Hongwei Zhou,Jianlong Wang

Journal of cell science 129:2407-15 PubMed27160682

2016

Sumoylation of eIF4A2 affects stress granule formation.

Applications

Unspecified application

Species

Unspecified reactive species

Jirapas Jongjitwimol,Robert A Baldock,Simon J Morley,Felicity Z Watts

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-eIF4A2 antibody

Western blot - Anti-eIF4A2 antibody (AB194471)

Western blot - Anti-eIF4A2 antibody (AB194471)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise