Rabbit Polyclonal eIF4A2 antibody. Suitable for IP, WB and reacts with Mouse, Human, Rat samples. Cited in 31 publications.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
IP | WB | |
---|---|---|
Human | Expected | Tested |
Mouse | Tested | Tested |
Rat | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Select an associated product type
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
DDX2B, EIF4F, EIF4A2, Eukaryotic initiation factor 4A-II, eIF-4A-II, eIF4A-II, ATP-dependent RNA helicase eIF4A-2
Rabbit Polyclonal eIF4A2 antibody. Suitable for IP, WB and reacts with Mouse, Human, Rat samples. Cited in 31 publications.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
The eIF4A2 also known as DDX2B functions as an RNA helicase with an ATP-dependent mechanism. It unwinds RNA secondary structures in the 5'UTR of mRNAs to facilitate ribosome binding. eIF4A2 belongs to the DEAD-box family of proteins and it has a molecular mass of approximately 46 kDa. It is ubiquitously expressed in various tissues indicating its broad role in cellular processes.
EIF4A2 is integral to the initiation of protein synthesis. It acts as a core member of the eIF4F complex along with the cap-binding protein eIF4E and scaffold protein eIF4G. This complex enables the recruitment of ribosomes to mRNA an important step in translation initiation. eIF4A2 helps regulate the translation of specific mRNAs that are important for cell growth and proliferation affecting how cells respond to environmental stimuli.
EIF4A2 plays a significant role in the mTOR and MAPK signaling pathways. In the mTOR pathway eIF4A2 controls the translation of mRNAs that drive cell growth and metabolism. It also interacts with other proteins such as mTORC1 and S6K which modulate these processes. Its involvement in the MAPK pathway links it with cell survival and stress responses emphasizing its interaction with proteins like MEK1/2 and ERK1/2 that further propagate these signals.
EIF4A2 has implications in cancer and neurodegenerative diseases. eIF4A2's overexpression has been associated with various cancers whereby it encourages oncogenic transformation through its role in synthesis of growth-related proteins. Moreover the helicase has links to Alzheimer's disease where it may influence disease progression by modulating synaptic plasticity proteins such as APP and tau important for neuronal function. The regulation by eIF4A2 of these proteins highlights its potential as a therapeutic target.
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All lanes: Western blot - Anti-eIF4A2 antibody (ab31218) at 1 µg/mL
All lanes: Western blot - Jurkat whole cell lysate (Jurkat whole cell lysate ab7899) at 20 µg
All lanes: IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 47 kDa, 52 kDa
eIF4A2 was immunoprecipitated using 0.5mg Mouse skeletal muscle whole tissue extract, 5μg of Rabbit polyclonal to eIF4A2 and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse skeletal muscle whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70oC; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31218.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) ab99697).
Band: 47kDa: eIF4A2 .
All lanes: Immunoprecipitation - Anti-eIF4A2 antibody (ab31218)
Predicted band size: 46 kDa
All lanes: Western blot - Anti-eIF4A2 antibody (ab31218) at 1 µg/mL
Lane 1: Brain (Mouse) Tissue Lysate at 10 µg
Lane 2: Testis (Mouse) Tissue Lysate at 10 µg
Lane 3: Western blot - Mouse skeletal muscle tissue lysate - total protein (Mouse skeletal muscle tissue lysate - total protein ab29711) at 10 µg
Lane 4: Spinal Cord (Mouse) Tissue Lysate at 10 µg
Lane 5: Ovary (Mouse) Tissue Lysate at 10 µg
Lane 6: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 7: Brain (Rat) Tissue Lysate at 10 µg
Lane 8: Heart (Rat) Tissue Lysate at 10 µg
All lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 47 kDa, 51 kDa
Western blot: Anti-EIF4A2 antibody (ab31218) staining at 1 ug/ml, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab31218 was shown to bind specifically to EIF4A2. A band was observed at 40-50 kDa in wild-type A549 cell lysates with no signal observed at this size in EIF4A2 knockout cell line. To generate this image, wild-type and EIF4A2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-eIF4A2 antibody (ab31218) at 1 µg/mL
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: EIF4A2 knockout A549 cell lysate at 20 µg
Lane 3: MCF7 cell lysate at 20 µg
Lane 4: MCF7 Membrane Prep cell lysate at 20 µg
All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 40 kDa, 50 kDa
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