AB32485

Anti-eIF4A3 antibody

Name: Anti-eIF4A3 antibody (ab32485) | Abcam
Brand: Abcam Limited
SKU: AB32485
Rating: 4 (9 reviews)

(9 Reviews)

(34 Publications)

Rabbit Polyclonal eIF4A3 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Rat samples. Cited in 34 publications.

View Alternative Names

DDX48, KIAA0111, EIF4A3, Eukaryotic initiation factor 4A-III, eIF-4A-III, eIF4A-III, ATP-dependent RNA helicase DDX48, ATP-dependent RNA helicase eIF4A-3, DEAD box protein 48, Eukaryotic initiation factor 4A-like NUK-34, Eukaryotic translation initiation factor 4A isoform 3, Nuclear matrix protein 265, NMP 265, hNMP 265

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody (AB32485)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody (AB32485)

ICC/IF image of ab32485 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab32485, 5μg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Unknown

Immunoprecipitation - Anti-eIF4A3 antibody (AB32485)

eIF4A3 was immunoprecipitated using 0.5mg Hela whole cell extract, 5μg of Rabbit polyclonal to eIF4A3 and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).

The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70°C; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32485.

Secondary : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

Band : 47kDa; eIF4A3

All lanes:

Immunoprecipitation - Anti-eIF4A3 antibody (ab32485)

Predicted band size: 47 kDa

true

Exposure time: 20min

Project

Western blot - Anti-eIF4A3 antibody (AB32485)

All lanes:

Western blot - Anti-eIF4A3 antibody (ab32485) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg

Secondary

All lanes:

IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

Project

Western blot - Anti-eIF4A3 antibody (AB32485)

All lanes:

Western blot - Anti-eIF4A3 antibody (ab32485) at 1 µg/mL

Lane 1:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 2:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 47 kDa

Observed band size: 100 kDa,46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5 µg/mL", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF4A3 also known as DDX48 is an ATP-dependent RNA helicase. It unwinds RNA secondary structures in an energy-dependent manner being an important part in RNA metabolic processes. eIF4A3 has a molecular mass of approximately 46 kDa and is ubiquitously expressed in various tissues and cell types. It localizes in both the cytoplasm and nucleus indicating its involvement in diverse cellular functions.

Biological function summary

EIF4A3 engages in nonsense-mediated mRNA decay and plays a role in mRNA splicing. It is an important component of the exon junction complex (EJC) which includes other proteins like MAGOH and Y14. This complex ensures proper mRNA surveillance by marking transcripts that need to be subject to degradation if errors are found.

Pathways

EIF4A3 interacts with cellular processes such as mRNA surveillance and splicing. It integrates within the nonsense-mediated decay (NMD) pathway and the spliceosome-associated pathways. eIF4A3's activity closely relates to proteins like UPF1 and RNPS1 which coordinate the removal of defective mRNA and support mRNA splicing.

EIF4A3 has connections with cancer and neurodegenerative diseases. Aberrant expression of eIF4A3 links to tumor progression where its role in RNA processing becomes dysregulated. Moreover in neurodegenerative disorders like spinal muscular atrophy eIF4A3 interacts with proteins such as SMN leading to deficits in mRNA processing which contributes to disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATP-dependent RNA helicase (PubMed : 16170325). Involved in pre-mRNA splicing as component of the spliceosome (PubMed : 11991638, PubMed : 22961380, PubMed : 28076346, PubMed : 28502770, PubMed : 29301961). Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs (PubMed : 16170325, PubMed : 16209946, PubMed : 16314458, PubMed : 16923391, PubMed : 16931718, PubMed : 19033377, PubMed : 20479275). The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly (PubMed : 22203037). Involved in craniofacial development (PubMed : 24360810).

See full target information EIF4A3

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Publications (34)

Recent publications for all applications. Explore the full list and refine your search

World journal of diabetes 16:105173 PubMed40548267

2025

Mechanism of the epidermal growth factor receptor in promoting endothelial cell dysfunction in gestational diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Tang,Cheng-Fen Wang,Jue Wang,Xiao-Tao Jing,Jing Ma

Scientific reports 15:19979 PubMed40481141

2025

EIF4A3 enhances the viability, invasion and osteogenic differentiation of BMSCs via the USP53/SMAD5 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Cheng,Xiaoling Yang,Yongtao Tang,Changjun Wu,Zulin Tang,Siqi Wei

Nature communications 16:2434 PubMed40069174

2025

The DEAD-box helicase eIF4A1/2 acts as RNA chaperone during mitotic exit enabling chromatin decondensation.

Applications

Unspecified application

Species

Unspecified reactive species

Ramona Jühlen,Sabine C Wiesmann,Anja Scheufen,Thilo Stausberg,Isabel Braun,Chantal Strobel,Carmen Llera-Brandt,Sabrina Rappold,Rabia Suluyayla,Marianna Tatarek-Nossol,Birgitt Lennartz,Hongqi Lue,Maximilian W G Schneider,Juan-Felipe Perez-Correa,Daniel Moreno-Andrés,Wolfram Antonin

Journal of cellular and molecular medicine 28:e70098 PubMed39307884

2024

Inhibition of circular JUN prevents the proliferation and invasion of glioblastoma via miR-3064-IGFBP5 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhao Zhang,Shiming Liu,Cheng Wu,Xin Gao,Hongtao Zhao,Ou Li,Faliang Gao

Cell reports 43:114666 PubMed39182224

2024

The RNA-binding protein EIF4A3 promotes axon development by direct control of the cytoskeleton.

Applications

Unspecified application

Species

Unspecified reactive species

Fernando C Alsina,Bianca M Lupan,Lydia J Lin,Camila M Musso,Federica Mosti,Carly R Newman,Lisa M Wood,Aussie Suzuki,Mark Agostino,Jeffrey K Moore,Debra L Silver

Translational vision science & technology 13:12 PubMed38587436

2024

Role of Hsa_circ_0000880 in the Regulation of High Glucose-Induced Apoptosis of Retinal Microvascular Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiawei Wang,Nannan Yang,Wanna Li,Han Zhang,Jianqiao Li

Molecular medicine (Cambridge, Mass.) 29:155 PubMed37940881

2023

Exosomal circCOL1A1 promotes angiogenesis via recruiting EIF4A3 protein and activating Smad2/3 pathway in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Gui Hu,Changwei Lin,Kai Gao,Miao Chen,Fei Long,Buning Tian

Journal of biochemical and molecular toxicology 38:e23530 PubMed37822284

2023

CircDLGAP4 overexpression ameliorates neuronal injury in Parkinson's disease by binding to EIF4A3 and increasing HMGA2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Haiping Bao,Qiang Zhang,Yu Li,Chen Nie

Cell death discovery 9:161 PubMed37179359

2023

Circ_0020256 induces fibroblast activation to drive cholangiocarcinoma development via recruitment of EIF4A3 protein to stabilize KLF4 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Zongyan Li,Zuxiao Chen,Shiying Li,Xiangjun Qian,Lei Zhang,Guojie Long,Jiancong Xie,Xiaoming Huang,Zheyu Zheng,Weidong Pan,Haiyan Li,Dawei Zhang

The Kaohsiung journal of medical sciences 39:478-488 PubMed37098796

2023

Promotive role of eukaryotic translation initiation factor 4A isoform 3 in ovarian cancer cell growth and aerobic glycolysis through the pyruvate dehydrogenase kinase 4 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Sha-Sha Bai,Li-Wei Yan,Chun-Hui Liu

View all publications

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Anti-eIF4A3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody (AB32485)

Immunoprecipitation - Anti-eIF4A3 antibody (AB32485)

Western blot - Anti-eIF4A3 antibody (AB32485)

Western blot - Anti-eIF4A3 antibody (AB32485)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (34)

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