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Rabbit Recombinant Monoclonal eIF4A3 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 12 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] (AB180573), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] (AB180573), expandable thumbnail
  • Western blot - Anti-eIF4A3 antibody [EPR14301(B)] (AB180573), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] (AB180573), expandable thumbnail
  • Immunoprecipitation - Anti-eIF4A3 antibody [EPR14301(B)] (AB180573), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Predicted
Predicted
Chicken
Predicted
Predicted
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Predicted
Predicted
Cynomolgus monkey
Predicted
Predicted
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted
Predicted
Predicted
Xenopus laevis
Predicted
Predicted
Predicted
Predicted
Predicted
Xenopus tropicalis
Predicted
Predicted
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Cynomolgus monkey, Xenopus tropicalis
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/2000
Notes

-

Predicted
Predicted

Species
Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Cynomolgus monkey, Xenopus tropicalis
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Predicted
Predicted

Species
Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Cynomolgus monkey, Xenopus tropicalis
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/160
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Cynomolgus monkey, Xenopus tropicalis
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Cynomolgus monkey, Xenopus tropicalis
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

ATP-dependent RNA helicase (PubMed:16170325). Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:22961380, PubMed:28076346, PubMed:28502770, PubMed:29301961). Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs (PubMed:16170325, PubMed:16209946, PubMed:16314458, PubMed:16923391, PubMed:16931718, PubMed:19033377, PubMed:20479275). The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly (PubMed:22203037). Involved in craniofacial development (PubMed:24360810).

Alternative names

DDX48, KIAA0111, EIF4A3, Eukaryotic initiation factor 4A-III, eIF-4A-III, eIF4A-III, ATP-dependent RNA helicase DDX48, ATP-dependent RNA helicase eIF4A-3, DEAD box protein 48, Eukaryotic initiation factor 4A-like NUK-34, Eukaryotic translation initiation factor 4A isoform 3, Nuclear matrix protein 265, NMP 265, hNMP 265

Recommended products

Rabbit Recombinant Monoclonal eIF4A3 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 12 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR14301(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EIF4A3 also known as DDX48 is an ATP-dependent RNA helicase. It unwinds RNA secondary structures in an energy-dependent manner being an important part in RNA metabolic processes. eIF4A3 has a molecular mass of approximately 46 kDa and is ubiquitously expressed in various tissues and cell types. It localizes in both the cytoplasm and nucleus indicating its involvement in diverse cellular functions.

Biological function summary

EIF4A3 engages in nonsense-mediated mRNA decay and plays a role in mRNA splicing. It is an important component of the exon junction complex (EJC) which includes other proteins like MAGOH and Y14. This complex ensures proper mRNA surveillance by marking transcripts that need to be subject to degradation if errors are found.

Pathways

EIF4A3 interacts with cellular processes such as mRNA surveillance and splicing. It integrates within the nonsense-mediated decay (NMD) pathway and the spliceosome-associated pathways. eIF4A3's activity closely relates to proteins like UPF1 and RNPS1 which coordinate the removal of defective mRNA and support mRNA splicing.

Associated diseases and disorders

EIF4A3 has connections with cancer and neurodegenerative diseases. Aberrant expression of eIF4A3 links to tumor progression where its role in RNA processing becomes dysregulated. Moreover in neurodegenerative disorders like spinal muscular atrophy eIF4A3 interacts with proteins such as SMN leading to deficits in mRNA processing which contributes to disease pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

    Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling eIF4A3 with ab180573 at 1/100 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

    Immunofluorescent analysis of 4% paraformaldehyde MCF7 cells labeling eIF4A3 with ab180573 at 1/500 dilution (green), or with Dapi counter stain (blue). Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody, at a dilution of 1/200.

  • Western blot - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573), expandable thumbnail

    Western blot - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

    Blocking and dilution buffer:5% NFDM/TBST.

    Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606) used as loading control at 1/1000 dilution. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP goat anti-rabbit IgG (H+L), at a dilution of 1/20000.

    All lanes: Western blot - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573) at 1/1000 dilution

    Lane 1: eIF4A1 Human Recombinant Protein at 10 ng

    Lane 2: eIF4A2 Human Recombinant Protein at 10 ng

    Lane 3: eIF4A3 Human Recombinant Protein at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 47 kDa

    Observed band size: 46 kDa

    Exposure time: 5s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

    Immunohistochemical analysis of formalin fixed paraffin embedded Human spleen tissue labelling eIF4A3 with ab180573 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Sections were counterstained with Hematoxylin.

    Positive staining on human spleen.

    The section was incubated with ab180573 at 4°C overnight.

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH9.0)

  • Immunoprecipitation - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573), expandable thumbnail

    Immunoprecipitation - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

    eIF4A3 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab180573 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab180573 at 1/500 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate, 10 µg
    Lane 2: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab180573 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573) at 1/500 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

    Lane 2: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab180573 in HeLa whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Predicted band size: 47 kDa

    Exposure time: 1s

  • Western blot - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573), expandable thumbnail

    Western blot - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

    Blocking and diluting buffer and concentration: 5% NFDM /TBST

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.

    ab180573 has a faint band above the target band, we recommend using Anti-eIF4A3 antibody [EPR14302(B)] ab180519 to get desire result in western blot.

    All lanes: Western blot - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573) at 1/1000 dilution

    Lane 1: SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysate at 20 µg

    Lane 2: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 3: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

    Lane 7: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 47 kDa

    Observed band size: 46 kDa

  • Flow Cytometry (Intracellular) - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling eIF4A3 with ab180573 at 1/90 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed ab150097) at 1/5000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] (ab180573)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling eIF4A3 with ab180573 at 1/100 (9.39 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Nuclear DNA was labelled with DAPI (shown in blue). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red).

    Confocal image showing nuclear staining in HeLa cells.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

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Product protocols

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