Rabbit Recombinant Monoclonal eIF4A3 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (AB236131), expandable thumbnail](https://content.abcam.com/products/images/eif4a3-antibody-epr14301b-bsa-and-azide-free-ab236131--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img54566.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (AB236131)")
![Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (AB236131), expandable thumbnail](https://content.abcam.com/products/images/eif4a3-antibody-epr14301b-bsa-and-azide-free-ab236131--immunocytochemistry-immunofluorescence-img13834.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (AB236131)")
![Western blot - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (AB236131), expandable thumbnail](https://content.abcam.com/products/images/eif4a3-antibody-epr14301b-bsa-and-azide-free-ab236131--western-blot-img436494.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (AB236131)")
![Flow Cytometry (Intracellular) - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (AB236131), expandable thumbnail](https://content.abcam.com/products/images/eif4a3-antibody-epr14301b-bsa-and-azide-free-ab236131--flow-cytometry-intracellular-img436491.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (AB236131)")
![Western blot - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (AB236131), expandable thumbnail](https://content.abcam.com/products/images/eif4a3-antibody-epr14301b-bsa-and-azide-free-ab236131--western-blot-img431690.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (AB236131)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted | Predicted | Predicted | Predicted |
| Zebrafish | Predicted | Predicted | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Cynomolgus monkey, Xenopus tropicalis | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Cynomolgus monkey, Xenopus tropicalis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Cynomolgus monkey, Xenopus tropicalis | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Cynomolgus monkey, Xenopus tropicalis | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Pig, Xenopus laevis, Zebrafish, Cynomolgus monkey, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Version
2 products for Alternative Product
Target data
Function
ATP-dependent RNA helicase (PubMed:16170325). Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:22961380, PubMed:28076346, PubMed:28502770, PubMed:29301961). Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs (PubMed:16170325, PubMed:16209946, PubMed:16314458, PubMed:16923391, PubMed:16931718, PubMed:19033377, PubMed:20479275). The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly (PubMed:22203037). Involved in craniofacial development (PubMed:24360810).
Alternative names
DDX48, KIAA0111, EIF4A3, Eukaryotic initiation factor 4A-III, eIF-4A-III, eIF4A-III, ATP-dependent RNA helicase DDX48, ATP-dependent RNA helicase eIF4A-3, DEAD box protein 48, Eukaryotic initiation factor 4A-like NUK-34, Eukaryotic translation initiation factor 4A isoform 3, Nuclear matrix protein 265, NMP 265, hNMP 265
Recommended products
Rabbit Recombinant Monoclonal eIF4A3 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR14301(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab236131 is the carrier-free version of Anti-eIF4A3 antibody [EPR14301(B)] ab180573.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EIF4A3 also known as DDX48 is an ATP-dependent RNA helicase. It unwinds RNA secondary structures in an energy-dependent manner being an important part in RNA metabolic processes. eIF4A3 has a molecular mass of approximately 46 kDa and is ubiquitously expressed in various tissues and cell types. It localizes in both the cytoplasm and nucleus indicating its involvement in diverse cellular functions.
- Biological function summary
EIF4A3 engages in nonsense-mediated mRNA decay and plays a role in mRNA splicing. It is an important component of the exon junction complex (EJC) which includes other proteins like MAGOH and Y14. This complex ensures proper mRNA surveillance by marking transcripts that need to be subject to degradation if errors are found.
- Pathways
EIF4A3 interacts with cellular processes such as mRNA surveillance and splicing. It integrates within the nonsense-mediated decay (NMD) pathway and the spliceosome-associated pathways. eIF4A3's activity closely relates to proteins like UPF1 and RNPS1 which coordinate the removal of defective mRNA and support mRNA splicing.
- Associated diseases and disorders
EIF4A3 has connections with cancer and neurodegenerative diseases. Aberrant expression of eIF4A3 links to tumor progression where its role in RNA processing becomes dysregulated. Moreover in neurodegenerative disorders like spinal muscular atrophy eIF4A3 interacts with proteins such as SMN leading to deficits in mRNA processing which contributes to disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131), expandable thumbnail](https://content.abcam.com/products/images/eif4a3-antibody-epr14301b-bsa-and-azide-free-ab236131--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img54566.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling eIF4A3 with Anti-eIF4A3 antibody [EPR14301(B)] ab180573 at 1/100 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-eIF4A3 antibody [EPR14301(B)] ab180573).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131), expandable thumbnail](https://content.abcam.com/products/images/eif4a3-antibody-epr14301b-bsa-and-azide-free-ab236131--immunocytochemistry-immunofluorescence-img13834.jpg "Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)")
Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)
Immunofluorescent analysis of 4% paraformaldehyde MCF7 cells labeling eIF4A3 with Anti-eIF4A3 antibody [EPR14301(B)] ab180573 at 1/500 dilution (green), or with Dapi counter stain (blue). Goat anti rabbit IgG (Dylight 488) was used as a secondary antibody, at a dilution of 1/200.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-eIF4A3 antibody [EPR14301(B)] ab180573).
![Western blot - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131), expandable thumbnail](https://content.abcam.com/products/images/eif4a3-antibody-epr14301b-bsa-and-azide-free-ab236131--western-blot-img436494.jpg "Western blot - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)")
Western blot - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-eIF4A3 antibody [EPR14301(B)] ab180573).
Blocking and diluting buffer and concentration: 5% NFDM /TBST
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.
Anti-eIF4A3 antibody [EPR14301(B)] ab180573 has a faint band above the target band, we recommend using Anti-eIF4A3 antibody [EPR14302(B)] ab180519 to get desire result in western blot.
All lanes: Western blot - Anti-eIF4A3 antibody [EPR14301(B)] (Anti-eIF4A3 antibody [EPR14301(B)] ab180573) at 1/1000 dilution
Lane 1: SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysate at 20 µg
Lane 2: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 3: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 6: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg
Lane 7: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 46 kDa
![Flow Cytometry (Intracellular) - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131), expandable thumbnail](https://content.abcam.com/products/images/eif4a3-antibody-epr14301b-bsa-and-azide-free-ab236131--flow-cytometry-intracellular-img436491.jpg "Flow Cytometry (Intracellular) - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)")
Flow Cytometry (Intracellular) - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-eIF4A3 antibody [EPR14301(B)] ab180573).
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling eIF4A3 with Anti-eIF4A3 antibody [EPR14301(B)] ab180573 at 1/90 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed ab150097) at 1/5000 dilution was used as the secondary antibody.
![Western blot - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131), expandable thumbnail](https://content.abcam.com/products/images/eif4a3-antibody-epr14301b-bsa-and-azide-free-ab236131--western-blot-img431690.jpg "Western blot - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)")
Western blot - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)
Blocking and dilution buffer:5% NFDM/TBST.Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606) used as loading control at 1/1000 dilution. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP goat anti-rabbit IgG (H+L), at a dilution of 1/20000.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-eIF4A3 antibody [EPR14301(B)] ab180573).
All lanes: Western blot - Anti-eIF4A3 antibody [EPR14301(B)] (Anti-eIF4A3 antibody [EPR14301(B)] ab180573) at 1/1000 dilution
Lane 1: eIF4A1 Human Recombinant Protein at 10 ng
Lane 2: eIF4A2 Human Recombinant Protein at 10 ng
Lane 3: eIF4A3 Human Recombinant Protein at 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 46 kDa
Exposure time: 5s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131), expandable thumbnail](https://content.abcam.com/products/images/eif4a3-antibody-epr14301b-bsa-and-azide-free-ab236131--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436493.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-eIF4A3 antibody [EPR14301(B)] ab180573).
Immunohistochemical analysis of formalin fixed paraffin embedded Human spleen tissue labelling eIF4A3 with Anti-eIF4A3 antibody [EPR14301(B)] ab180573 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Sections were counterstained with Hematoxylin.
Positive staining on human spleen.
The section was incubated with Anti-eIF4A3 antibody [EPR14301(B)] ab180573 at 4°C overnight.Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH9.0)
![Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131), expandable thumbnail](https://content.abcam.com/products/images/eif4a3-antibody-epr14301b-bsa-and-azide-free-ab236131--immunocytochemistry-immunofluorescence-img436492.jpg "Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)")
Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-eIF4A3 antibody [EPR14301(B)] ab180573).
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling eIF4A3 with Anti-eIF4A3 antibody [EPR14301(B)] ab180573 at 1/100 (9.39 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Nuclear DNA was labelled with DAPI (shown in blue). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red).
Confocal image showing nuclear staining in HeLa cells.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
![Immunoprecipitation - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131), expandable thumbnail](https://content.abcam.com/products/images/eif4a3-antibody-epr14301b-bsa-and-azide-free-ab236131--immunoprecipitation-img436490.jpg "Immunoprecipitation - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)")
Immunoprecipitation - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-eIF4A3 antibody [EPR14301(B)] ab180573).
eIF4A3 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with Anti-eIF4A3 antibody [EPR14301(B)] ab180573 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-eIF4A3 antibody [EPR14301(B)] ab180573 at 1/500 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate, 10 µg
Lane 2: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-eIF4A3 antibody [EPR14301(B)] ab180573 in HeLa whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST
All lanes: Immunoprecipitation - Anti-eIF4A3 antibody [EPR14301(B)] (Anti-eIF4A3 antibody [EPR14301(B)] ab180573) at 1/500 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg
Lane 2: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-eIF4A3 antibody [EPR14301(B)] ab180573 in HeLa whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Predicted band size: 47 kDa
Exposure time: 1s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131), expandable thumbnail](https://content.abcam.com/products/images/eif4a3-antibody-epr14301b-bsa-and-azide-free-ab236131--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436493.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)")
![Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131), expandable thumbnail](https://content.abcam.com/products/images/eif4a3-antibody-epr14301b-bsa-and-azide-free-ab236131--immunocytochemistry-immunofluorescence-img436492.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)")
![Immunoprecipitation - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131), expandable thumbnail](https://content.abcam.com/products/images/eif4a3-antibody-epr14301b-bsa-and-azide-free-ab236131--immunoprecipitation-img436490.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-eIF4A3 antibody [EPR14301(B)] - BSA and Azide free (ab236131)")
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