AB308024

Anti-eIF4A3 antibody [RP23040208]

Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Multiclonal eIF4A3 antibody. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human EIF4A3.

View Alternative Names

DDX48, KIAA0111, EIF4A3, Eukaryotic initiation factor 4A-III, eIF-4A-III, eIF4A-III, ATP-dependent RNA helicase DDX48, ATP-dependent RNA helicase eIF4A-3, DEAD box protein 48, Eukaryotic initiation factor 4A-like NUK-34, Eukaryotic translation initiation factor 4A isoform 3, Nuclear matrix protein 265, NMP 265, hNMP 265

1 Images

Supplier Data

Western blot - Anti-eIF4A3 antibody [RP23040208] (AB308024)

Western blot analysis was performed on Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), Raji (Lane 2), Hep G2 (Lane 3), C2C12 (Lane 4), CaCO2 (Lane 5) and PC3 (Lane 6). The blots were probed with ab308024, 1-2 µg/mL and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.4 µg/mL, 1 : 2500 dilution). A 48 kDa band corresponding to DDX48 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).

All lanes:

Western blot - Anti-eIF4A3 antibody [RP23040208] (ab308024) at 1 µg/mL

Lane 1:

MCF7 cell lysate at 30 µg

Lane 2:

Raji cell lysate at 30 µg

Lane 3:

Hep G2 cell lysate at 30 µg

Lane 4:

C2C12 cell lysate at 30 µg

Lane 5:

CaCo2 cell lysate at 30 µg

Lane 6:

PC3 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugate Goat anti-Rabbit IgG (H+L) Secondary Antibody at 0.4 µg/mL

Predicted band size: 47 kDa

Observed band size: 48 kDa

true

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040208

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human EIF4A3. Database link P38919

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

style

left-column

Properties and storage information

Form

Liquid

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF4A3 also known as DDX48 is an ATP-dependent RNA helicase. It unwinds RNA secondary structures in an energy-dependent manner being an important part in RNA metabolic processes. eIF4A3 has a molecular mass of approximately 46 kDa and is ubiquitously expressed in various tissues and cell types. It localizes in both the cytoplasm and nucleus indicating its involvement in diverse cellular functions.

Biological function summary

EIF4A3 engages in nonsense-mediated mRNA decay and plays a role in mRNA splicing. It is an important component of the exon junction complex (EJC) which includes other proteins like MAGOH and Y14. This complex ensures proper mRNA surveillance by marking transcripts that need to be subject to degradation if errors are found.

Pathways

EIF4A3 interacts with cellular processes such as mRNA surveillance and splicing. It integrates within the nonsense-mediated decay (NMD) pathway and the spliceosome-associated pathways. eIF4A3's activity closely relates to proteins like UPF1 and RNPS1 which coordinate the removal of defective mRNA and support mRNA splicing.

EIF4A3 has connections with cancer and neurodegenerative diseases. Aberrant expression of eIF4A3 links to tumor progression where its role in RNA processing becomes dysregulated. Moreover in neurodegenerative disorders like spinal muscular atrophy eIF4A3 interacts with proteins such as SMN leading to deficits in mRNA processing which contributes to disease pathology.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

ATP-dependent RNA helicase (PubMed : 16170325). Involved in pre-mRNA splicing as component of the spliceosome (PubMed : 11991638, PubMed : 22961380, PubMed : 28076346, PubMed : 28502770, PubMed : 29301961). Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs (PubMed : 16170325, PubMed : 16209946, PubMed : 16314458, PubMed : 16923391, PubMed : 16931718, PubMed : 19033377, PubMed : 20479275). The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly (PubMed : 22203037). Involved in craniofacial development (PubMed : 24360810).

See full target information EIF4A3

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-eIF4A3 antibody [RP23040208]

Western blot - Anti-eIF4A3 antibody [RP23040208] (AB308024)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise