AB129369

Anti-eIF4B antibody [1F5]

Name: Anti-eIF4B antibody [1F5] (ab129369) | Abcam
Brand: Abcam Limited
SKU: AB129369

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(1 Publication)

Mouse Monoclonal eIF4B antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EIF4B.

View Alternative Names

Eukaryotic translation initiation factor 4B, eIF-4B, EIF4B

4 Images

Flow Cytometry - Anti-eIF4B antibody [1F5] (AB129369)

Flow Cyt

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Flow Cytometry - Anti-eIF4B antibody [1F5] (AB129369)

ab129369 at 1/200 dilution (green) or negative control (red) staining eIF4B in HeLa cells by flow cytometry.

Immunocytochemistry/ Immunofluorescence - Anti-eIF4B antibody [1F5] (AB129369)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-eIF4B antibody [1F5] (AB129369)

ab129369 at 1/200 dilution staining eIF4B in NIH3T3 cells by immunofluorescence. DNA was stained with DRAQ5 dye (blue) and actin with Alexa 555 phalloidin (red).

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Western blot - Anti-eIF4B antibody [1F5] (AB129369)

All lanes:

Western blot - Anti-eIF4B antibody [1F5] (ab129369) at 1/500 dilution

Lane 1:

A549 cell lysate

Lane 2:

A431 cell lysate

Lane 3:

HepG2 cell lysate

Lane 4:

HEK293 cell lysate

Lane 5:

HeLa cell lysate

Lane 6:

Jurkat cell lysate

Lane 7:

K562 cell lysate

Lane 8:

NIH3T3 cell lysate

Lane 9:

MCF-7 cell lysate

Predicted band size: 69 kDa

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Supplier Data

Western blot - Anti-eIF4B antibody [1F5] (AB129369)

The expected molecular weight of the recombinant fragment is 48 kDa.

All lanes:

Western blot - Anti-eIF4B antibody [1F5] (ab129369) at 1/500 dilution

All lanes:

eIF4B recombinant fragment (aa 381-585)

Predicted band size: 69 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1F5

Isotype

IgG2b

Carrier free

Reacts with

Mouse, Human

Applications

WB, Flow Cyt, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EIF4B. The exact immunogen used to generate this antibody is proprietary information.

P23588

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

This product was changed from ascites to supernatant. Lot no's high than GR185156-5 are from Tissue Culture Supernatant

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF4B also known as eukaryotic translation initiation factor 4B is a protein that enhances the RNA helicase activity of the eIF4A protein during the initiation of translation. It weighs approximately 80 kDa. eIF4B interacts directly with RNA stimulating the unwinding of mRNA secondary structures allowing the ribosome to access the mRNA strand for translation. It is expressed in most cell types playing an essential role in efficient protein synthesis.

Biological function summary

EIF4B supports the recruitment of ribosomal subunits to mRNA by interacting with eIF3 and the large ribosomal subunit. It forms part of the larger eIF4F complex which is important for the cap-dependent initiation of translation. By working with eIF4A and eIF4G eIF4B facilitates the scanning process of the ribosome to the start codon on mRNA leading to proper protein translation.

Pathways

EIF4B participates in the mTOR signaling pathway a central regulator of cellular growth and metabolism. It also contributes to the PI3K/AKT/mTOR pathway affecting cell proliferation and survival. Additionally eIF4B interacts with other proteins like eIF4E and eIF5 linking its function to these pathways and ensuring effective coordination of translation initiation as well as regulation under various conditions.

Aberrant eIF4B activity has connections to cancer and neurodegenerative diseases. Overexpression or dysregulation of eIF4B is often observed in various cancers due to its role in promoting protein synthesis necessary for tumor growth. It also indirectly interacts with oncogenic proteins such as mTOR highlighting its importance in cancer. In neurodegenerative disorders eIF4B’s role in protein homeostasis is disrupted contributing to disease progression alongside pathways involving proteins like eIF4A.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

See full target information EIF4B

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The international journal of biochemistry & cell b 104:73-86 PubMed30236993

2018

A MAPK/c-Jun-mediated switch regulates the initial adaptive and cell death responses to mitochondrial damage in a neuronal cell model.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas A Ryan,Katherine M Roper,Jacquelyn Bond,Sandra M Bell,Sean T Sweeney,Ewan E Morrison

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-eIF4B antibody [1F5]

Flow Cytometry - Anti-eIF4B antibody [1F5] (AB129369)

Immunocytochemistry/ Immunofluorescence - Anti-eIF4B antibody [1F5] (AB129369)

Western blot - Anti-eIF4B antibody [1F5] (AB129369)

Western blot - Anti-eIF4B antibody [1F5] (AB129369)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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