AB68474

Anti-eIF4B antibody [EP2299Y]

Name: Anti-eIF4B antibody [EP2299Y] (ab68474) | Abcam
Brand: Abcam Limited
SKU: AB68474
Price: 255 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
What is this?

(1 Review)

(4 Publications)

Rabbit Monoclonal eIF4B antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Eukaryotic translation initiation factor 4B, eIF-4B, EIF4B

3 Images

Flow Cytometry (Intracellular) - Anti-eIF4B antibody [EP2299Y] (AB68474)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-eIF4B antibody [EP2299Y] (AB68474)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling eIF4B with ab68474 at 1/200 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B antibody [EP2299Y] (AB68474)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B antibody [EP2299Y] (AB68474)

Human prostate adenocarcinoma stained with ab68474 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-eIF4B antibody [EP2299Y] (AB68474)

All lanes:

Western blot - Anti-eIF4B antibody [EP2299Y] (ab68474) at 1/200000 dilution

All lanes:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP conjugated Goat anti-rabbit. at 1/2000 dilution

Predicted band size: 69 kDa

Observed band size: 90 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2299Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF4B also known as eukaryotic translation initiation factor 4B is a protein that enhances the RNA helicase activity of the eIF4A protein during the initiation of translation. It weighs approximately 80 kDa. eIF4B interacts directly with RNA stimulating the unwinding of mRNA secondary structures allowing the ribosome to access the mRNA strand for translation. It is expressed in most cell types playing an essential role in efficient protein synthesis.

Biological function summary

EIF4B supports the recruitment of ribosomal subunits to mRNA by interacting with eIF3 and the large ribosomal subunit. It forms part of the larger eIF4F complex which is important for the cap-dependent initiation of translation. By working with eIF4A and eIF4G eIF4B facilitates the scanning process of the ribosome to the start codon on mRNA leading to proper protein translation.

Pathways

EIF4B participates in the mTOR signaling pathway a central regulator of cellular growth and metabolism. It also contributes to the PI3K/AKT/mTOR pathway affecting cell proliferation and survival. Additionally eIF4B interacts with other proteins like eIF4E and eIF5 linking its function to these pathways and ensuring effective coordination of translation initiation as well as regulation under various conditions.

Aberrant eIF4B activity has connections to cancer and neurodegenerative diseases. Overexpression or dysregulation of eIF4B is often observed in various cancers due to its role in promoting protein synthesis necessary for tumor growth. It also indirectly interacts with oncogenic proteins such as mTOR highlighting its importance in cancer. In neurodegenerative disorders eIF4B’s role in protein homeostasis is disrupted contributing to disease progression alongside pathways involving proteins like eIF4A.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

See full target information EIF4B

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 52:5356-5375 PubMed38366783

2024

Nuclear RNA-related processes modulate the assembly of cytoplasmic RNA granules.

Applications

Unspecified application

Species

Unspecified reactive species

Mor Angel,Eden Fleshler,Mohammad Khaled Atrash,Noa Kinor,Jennifer I C Benichou,Yaron Shav-Tal

Journal of cell science 135: PubMed35713120

2022

Glucocorticoids enhance chemotherapy-driven stress granule assembly and impair granule dynamics, leading to cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Avital Schwed-Gross,Hila Hamiel,Gabriel P Faber,Mor Angel,Rakefet Ben-Yishay,Jennifer I C Benichou,Dana Ishay-Ronen,Yaron Shav-Tal

Molecular cell 82:1557-1572.e7 PubMed35180429

2022

Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stress.

Applications

Unspecified application

Species

Unspecified reactive species

Mark Stoneley,Robert F Harvey,Thomas E Mulroney,Ryan Mordue,Rebekah Jukes-Jones,Kelvin Cain,Kathryn S Lilley,Ritwick Sawarkar,Anne E Willis

Journal of virology 88:6611-22 PubMed24696465

2014

The stress granule component TIA-1 binds tick-borne encephalitis virus RNA and is recruited to perinuclear sites of viral replication to inhibit viral translation.

Applications

ICC/IF

Species

Human

Amelina Albornoz,Tea Carletti,Gianmarco Corazza,Alessandro Marcello

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-eIF4B antibody [EP2299Y]

Flow Cytometry (Intracellular) - Anti-eIF4B antibody [EP2299Y] (AB68474)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B antibody [EP2299Y] (AB68474)

Western blot - Anti-eIF4B antibody [EP2299Y] (AB68474)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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