AB284714

Anti-eIF4B antibody [EP2299Y] - BSA and Azide free

RabMAb
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Rabbit Monoclonal eIF4B antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Eukaryotic translation initiation factor 4B, eIF-4B, EIF4B

3 Images

Flow Cytometry (Intracellular) - Anti-eIF4B antibody [EP2299Y] - BSA and Azide free (AB284714)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-eIF4B antibody [EP2299Y] - BSA and Azide free (AB284714)

This data was developed using ab68474, the same antibody clone in a different buffer formulation.

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling eIF4B with ab68474 at 1/200 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B antibody [EP2299Y] - BSA and Azide free (AB284714)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B antibody [EP2299Y] - BSA and Azide free (AB284714)

This data was developed using ab68474, the same antibody clone in a different buffer formulation.Human prostate adenocarcinoma stained with ab68474 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-eIF4B antibody [EP2299Y] - BSA and Azide free (AB284714)

This data was developed using ab68474, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-eIF4B antibody [EP2299Y] (<a href='/en-us/products/primary-antibodies/eif4b-antibody-ep2299y-ab68474'>ab68474</a>) at 1/200000 dilution

All lanes:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP conjugated Goat anti-rabbit. at 1/2000 dilution

Predicted band size: 69 kDa

Observed band size: 90 kDa

false

Unconjugated

Anti-eIF4B antibody [EP2299Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2299Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

ab284714 is the carrier-free version of ab68474

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF4B also known as eukaryotic translation initiation factor 4B is a protein that enhances the RNA helicase activity of the eIF4A protein during the initiation of translation. It weighs approximately 80 kDa. eIF4B interacts directly with RNA stimulating the unwinding of mRNA secondary structures allowing the ribosome to access the mRNA strand for translation. It is expressed in most cell types playing an essential role in efficient protein synthesis.

Biological function summary

EIF4B supports the recruitment of ribosomal subunits to mRNA by interacting with eIF3 and the large ribosomal subunit. It forms part of the larger eIF4F complex which is important for the cap-dependent initiation of translation. By working with eIF4A and eIF4G eIF4B facilitates the scanning process of the ribosome to the start codon on mRNA leading to proper protein translation.

Pathways

EIF4B participates in the mTOR signaling pathway a central regulator of cellular growth and metabolism. It also contributes to the PI3K/AKT/mTOR pathway affecting cell proliferation and survival. Additionally eIF4B interacts with other proteins like eIF4E and eIF5 linking its function to these pathways and ensuring effective coordination of translation initiation as well as regulation under various conditions.

Aberrant eIF4B activity has connections to cancer and neurodegenerative diseases. Overexpression or dysregulation of eIF4B is often observed in various cancers due to its role in promoting protein synthesis necessary for tumor growth. It also indirectly interacts with oncogenic proteins such as mTOR highlighting its importance in cancer. In neurodegenerative disorders eIF4B’s role in protein homeostasis is disrupted contributing to disease progression alongside pathways involving proteins like eIF4A.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

See full target information EIF4B

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-eIF4B antibody [EP2299Y] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-eIF4B antibody [EP2299Y] - BSA and Azide free (AB284714)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B antibody [EP2299Y] - BSA and Azide free (AB284714)

Western blot - Anti-eIF4B antibody [EP2299Y] - BSA and Azide free (AB284714)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise