AB59300

Anti-eIF4B (phospho S422) antibody

Name: Anti-eIF4B (phospho S422) antibody (ab59300) | Abcam
Brand: Abcam Limited
SKU: AB59300
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal eIF4B phospho S422 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Eukaryotic translation initiation factor 4B phospho S422 aa 350-450.

View Alternative Names

Eukaryotic translation initiation factor 4B, eIF-4B, EIF4B

3 Images

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Western blot - Anti-eIF4B (phospho S422) antibody (AB59300)

All lanes:

Western blot - Anti-eIF4B (phospho S422) antibody (ab59300) at 1/500 dilution

Lane 1:

extracts from NIH/3T3cells

Lane 2:

extracts from NIH/3T3cells with immunizing peptide

Predicted band size: 69 kDa

Observed band size: 69 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B (phospho S422) antibody (AB59300)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B (phospho S422) antibody (AB59300)

ab59300 at 1/50 dilution staining human colon carcinoma without (left) and with (right) immunizing peptide; paraffin embedded.

Immunocytochemistry/ Immunofluorescence - Anti-eIF4B (phospho S422) antibody (AB59300)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-eIF4B (phospho S422) antibody (AB59300)

ICC/IF image of ab59300 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59300, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human Eukaryotic translation initiation factor 4B phospho S422 aa 350-450. The exact immunogen used to generate this antibody is proprietary information.

P23588

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF4B also known as eukaryotic translation initiation factor 4B is a protein that enhances the RNA helicase activity of the eIF4A protein during the initiation of translation. It weighs approximately 80 kDa. eIF4B interacts directly with RNA stimulating the unwinding of mRNA secondary structures allowing the ribosome to access the mRNA strand for translation. It is expressed in most cell types playing an essential role in efficient protein synthesis.

Biological function summary

EIF4B supports the recruitment of ribosomal subunits to mRNA by interacting with eIF3 and the large ribosomal subunit. It forms part of the larger eIF4F complex which is important for the cap-dependent initiation of translation. By working with eIF4A and eIF4G eIF4B facilitates the scanning process of the ribosome to the start codon on mRNA leading to proper protein translation.

Pathways

EIF4B participates in the mTOR signaling pathway a central regulator of cellular growth and metabolism. It also contributes to the PI3K/AKT/mTOR pathway affecting cell proliferation and survival. Additionally eIF4B interacts with other proteins like eIF4E and eIF5 linking its function to these pathways and ensuring effective coordination of translation initiation as well as regulation under various conditions.

Aberrant eIF4B activity has connections to cancer and neurodegenerative diseases. Overexpression or dysregulation of eIF4B is often observed in various cancers due to its role in promoting protein synthesis necessary for tumor growth. It also indirectly interacts with oncogenic proteins such as mTOR highlighting its importance in cancer. In neurodegenerative disorders eIF4B’s role in protein homeostasis is disrupted contributing to disease progression alongside pathways involving proteins like eIF4A.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

See full target information Eukaryotic translation initiation factor 4B phospho S422

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer discovery 11:1228-1247 PubMed33328217

2020

MNK Inhibition Sensitizes -Mutant Colorectal Cancer to mTORC1 Inhibition by Reducing eIF4E Phosphorylation and c-MYC Expression.

Applications

Unspecified application

Species

Unspecified reactive species

John R P Knight,Constantinos Alexandrou,George L Skalka,Nikola Vlahov,Kathryn Pennel,Leah Officer,Ana Teodosio,Georgios Kanellos,David M Gay,Sebastian May-Wilson,Ewan M Smith,Arafath K Najumudeen,Kathryn Gilroy,Rachel A Ridgway,Dustin J Flanagan,Rachael C L Smith,Laura McDonald,Craig MacKay,Anne Cheasty,Kerri McArthur,Emma Stanway,Joshua D Leach,Rene Jackstadt,Joseph A Waldron,Andrew D Campbell,Georgios Vlachogiannis,Nicola Valeri,Kevin M Haigis,Nahum Sonenberg,Christopher G Proud,Neil P Jones,Martin E Swarbrick,Heather J McKinnon,William J Faller,John Le Quesne,Joanne Edwards,Anne E Willis,Martin Bushell,Owen J Sansom

BMC molecular biology 13:9 PubMed22436005

2012

Novel polysome messages and changes in translational activity appear after induction of adipogenesis in 3T3-L1 cells.

Applications

Species

Unspecified reactive species

Carolin Fromm-Dornieden,Silvia von der Heyde,Oleksandr Lytovchenko,Gabriela Salinas-Riester,Bertram Brenig,Tim Beissbarth,Bernhard G Baumgartner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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