JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB75823

Anti-eIF4B (phospho S504) antibody [EP2261Y]

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Monoclonal eIF4B phospho S504 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Eukaryotic translation initiation factor 4B, eIF-4B, EIF4B

1 Images
Western blot - Anti-eIF4B (phospho S504) antibody [EP2261Y] (AB75823)
  • WB

Unknown

Western blot - Anti-eIF4B (phospho S504) antibody [EP2261Y] (AB75823)

All lanes:

Western blot - Anti-eIF4B (phospho S504) antibody [EP2261Y] (ab75823) at 1/50000 dilution

Lane 1:

293T cell lysate, untreated at 10 µg

Lane 2:

293T cell lysate, treated with AP at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa

false

  • Carrier free

    Anti-eIF4B (phospho S504) antibody [EP2261Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2261Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB228800

Anti-eIF4B antibody - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-eIF4B antibody - N-terminal (AB228800)

  • 0
  • 0
Primary Antibodies

AB59300

Anti-eIF4B (phospho S422) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B (phospho S422) antibody (AB59300)

  • 0
  • 0
Primary Antibodies

AB129369

Anti-eIF4B antibody [1F5]

Flow Cytometry - Anti-eIF4B antibody [1F5] (AB129369)

  • 0
  • 0
Primary Antibodies

AB186856

Anti-eIF4B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B antibody (AB186856)

  • 0
  • 0
Primary Antibodies

AB245618

Anti-eIF4B antibody

Immunoprecipitation - Anti-eIF4B antibody (AB245618)

  • 0
  • 0
Primary Antibodies

AB68474

Anti-eIF4B antibody [EP2299Y]

RabMAb

Flow Cytometry (Intracellular) - Anti-eIF4B antibody [EP2299Y] (AB68474)

  • 5
  • 1
Primary Antibodies

AB134138

Anti-eIF4B (phospho S406) antibody [EPR7683]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4B (phospho S406) antibody [EPR7683] (AB134138)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB109268

Anti-mTOR (phospho S2448) antibody [EPR426(2)]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Frozen sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)

  • 4
  • 6
Primary Antibodies

AB176348

Anti-DDX39 antibody [EPR13507(B)]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-DDX39 antibody [EPR13507(B)] (AB176348)

  • 0
  • 0
Primary Antibodies

AB131210

Anti-ULK1 (phospho S623) antibody [EPR6154]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-ULK1 (phospho S623) antibody [EPR6154] (AB131210)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/25000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
style
left-column

Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF4B also known as eukaryotic translation initiation factor 4B is a protein that enhances the RNA helicase activity of the eIF4A protein during the initiation of translation. It weighs approximately 80 kDa. eIF4B interacts directly with RNA stimulating the unwinding of mRNA secondary structures allowing the ribosome to access the mRNA strand for translation. It is expressed in most cell types playing an essential role in efficient protein synthesis.
Biological function summary

EIF4B supports the recruitment of ribosomal subunits to mRNA by interacting with eIF3 and the large ribosomal subunit. It forms part of the larger eIF4F complex which is important for the cap-dependent initiation of translation. By working with eIF4A and eIF4G eIF4B facilitates the scanning process of the ribosome to the start codon on mRNA leading to proper protein translation.

Pathways

EIF4B participates in the mTOR signaling pathway a central regulator of cellular growth and metabolism. It also contributes to the PI3K/AKT/mTOR pathway affecting cell proliferation and survival. Additionally eIF4B interacts with other proteins like eIF4E and eIF5 linking its function to these pathways and ensuring effective coordination of translation initiation as well as regulation under various conditions.

Aberrant eIF4B activity has connections to cancer and neurodegenerative diseases. Overexpression or dysregulation of eIF4B is often observed in various cancers due to its role in promoting protein synthesis necessary for tumor growth. It also indirectly interacts with oncogenic proteins such as mTOR highlighting its importance in cancer. In neurodegenerative disorders eIF4B’s role in protein homeostasis is disrupted contributing to disease progression alongside pathways involving proteins like eIF4A.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
See full target information EIF4B pS504
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 12:769 PubMed34349120

2021

Casein Kinase 2 dependent phosphorylation of eIF4B regulates BACE1 expression in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Bettegazzi,Laura Sebastian Monasor,Serena Bellani,Franca Codazzi,Lisa Michelle Restelli,Alessio Vittorio Colombo,Nikolaus Deigendesch,Stephan Frank,Takashi Saito,Takaomi C Saido,Sven Lammich,Sabina Tahirovic,Fabio Grohovaz,Daniele Zacchetti

Scientific reports 7:10563 PubMed28874824

2017

eIF4B phosphorylation at Ser504 links synaptic activity with protein translation in physiology and pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Bettegazzi,Serena Bellani,Paolo Roncon,Fabrizia Claudia Guarnieri,Alice Bertero,Franca Codazzi,Flavia Valtorta,Michele Simonato,Fabio Grohovaz,Daniele Zacchetti
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com