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AB254593

Anti-EIF4E2 antibody

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Rabbit Polyclonal EIF4E2 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EIF4E2 aa 1-150.

View Alternative Names

EIF4EL3, EIF4E2, Eukaryotic translation initiation factor 4E type 2, eIF-4E type 2, eIF4E type 2, Eukaryotic translation initiation factor 4E homologous protein, Eukaryotic translation initiation factor 4E-like 3, eIF4E-like protein 4E-LP, mRNA cap-binding protein 4EHP, mRNA cap-binding protein type 3, h4EHP

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-EIF4E2 antibody (AB254593)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EIF4E2 antibody (AB254593)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling EIF4E2 using ab254593 at 4µg/ml (green) in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EIF4E2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O60573

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF4E2 also known as eIF4E homologous protein (4EHP) is a mechanistic player in translation initiation. As a part of the eukaryotic translation initiation factor family it has a mass of approximately 25 kDa. EIF4E2 predominantly locates in the cytoplasm and its expression levels vary but often arise in specific tissues such as the brain and placenta. It interacts with other components of translation machinery acting to regulate protein synthesis by modulating mRNA cap binding activity.
Biological function summary

EIF4E2 plays a role in mRNA translation regulation. It forms a complex with other initiation factors such as eIF4G but lacks some binding capabilities of its closely related factor eIF4E. EIF4E2 impacts translation repression contributing by silencing specific mRNAs in cell cycles or stress responses. The function of EIF4E2 is necessary for maintaining cellular homeostasis particularly in adapting to environmental or cellular stresses.

Pathways

EIF4E2 participates significantly in the mTOR signaling and hypoxia response pathways. EIF4E2's involvement in mTOR signaling overlaps with its relationship to target proteins like 4E-BP1 which regulate cell growth and proliferation. In the hypoxia response pathway EIF4E2's translation control role is critical for cellular adaptation to low oxygen conditions by modulating hypoxia-inducible factors (HIFs).

Research links EIF4E2 to cancer and neurological disorders. Its role in abnormal cellular growth ties it to various cancers where changes in translation rates contribute to oncogenesis. EIF4E2's regulatory function in mRNA translation also connects it to neurodegenerative conditions which often involve dysfunctions in protein synthesis. EIF4E2 interacts with related proteins such as eIF4E and 4E-BP1 in these pathologies pointing to translation control as a potential therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation. Acts as a repressor of translation initiation (PubMed : 17368478, PubMed : 22751931, PubMed : 25624349, PubMed : 33581076, PubMed : 9582349). In contrast to EIF4E, it is unable to bind eIF4G (EIF4G1, EIF4G2 or EIF4G3), suggesting that it acts by competing with EIF4E and block assembly of eIF4F at the cap (By similarity). In P-bodies, component of a complex that promotes miRNA-mediated translational repression (PubMed : 28487484). Involved in virus-induced host response by mediating miRNA MIR34A-induced translational silencing which controls IFNB1 production by a negative feedback mechanism (PubMed : 28487484, PubMed : 33581076).. Component of the 4EHP-GYF2 complex, a multiprotein complex that acts as a repressor of translation initiation (PubMed : 22751931, PubMed : 35878012). In association with GIGYF2, assists ribosome-associated quality control (RQC) by sequestering the mRNA cap, blocking ribosome initiation and decreasing the translational load on problematic messages. Part of a pathway that works in parallel to RQC-mediated degradation of the stalled nascent polypeptide. GIGYF2 and EIF4E2 work downstream and independently of ZNF598, which seems to work as a scaffold that can recruit them to faulty mRNA even if alternative recruitment mechanisms may exist (PubMed : 32726578).. (Microbial infection) Upon SARS coronavirus-2/SARS-CoV-2 infection, the interaction with non-structural protein 2 (nsp2) with GIGYF2 enhances GIGYF2 binding to EIF4E2 and increases repression of translation initiation of genes involved in antiviral innate immune response such as IFNB1.
See full target information EIF4E2
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Product promise

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