Rabbit Polyclonal eIF4EBP1 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 37 publications. Immunogen corresponding to Synthetic Peptide within Human EIF4EBP1.
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- PLoS biology 21:e30023552023Nonsense mutation suppression is enhanced by targeting different stages of the protein synthesis process.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmnon Wittenstein,Michal Caspi,Ido Rippin,Orna Elroy-Stein,Hagit Eldar-Finkelman,Sven Thoms,Rina Rosin-ArbesfeldPubMed 37943958
- Frontiers in nutrition 10:10987152023Sodium acetate regulates milk fat synthesis through the activation of GPR41/GPR43 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingao Qi,Tenghui Zheng,Xinghong Liu,Siwang Yang,Qihui Li,Jiayuan Shao,Xiangfang Zeng,Wutai Guan,Shihai ZhangPubMed 36969813
- Journal of animal science and technology 64:922-9362022Effect of serotonin on the cell viability of the bovine mammary alveolar cell-T (MAC-T) cell line.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXusheng Dong,Chen Liu,Jialin Miao,Xueyan Lin,Yun Wang,Zhonghua Wang,Qiuling HouPubMed 36287778
- Autophagy 19:1087-10992022NPPA/atrial natriuretic peptide is an extracellular modulator of autophagy in the heart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaurizio Forte,Simona Marchitti,Flavio Di Nonno,Rosita Stanzione,Leonardo Schirone,Maria Cotugno,Franca Bianchi,Sonia Schiavon,Salvatore Raffa,Danilo Ranieri,Salvatore Fioriniello,Floriana Della Ragione,Maria Rosaria Torrisi,Roberto Carnevale,Valentina Valenti,Francesco Versaci,Giacomo Frati,Carmine Vecchione,Massimo Volpe,Speranza Rubattu,Sebastiano SciarrettaPubMed 35998113
- Clinical, cosmetic and investigational dermatology 15:643-6512022Enhanced Activation of mTOR Signaling Pathway Was Found in the Hypertrophic and Nodular Lesions of Port Wine Stains.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng-Nan Xu,Qian Wang,Min Wang,Yuan Xu,Si-Ming YuanPubMed 35444442
- Oncology letters 23:702022CRNDE silencing promotes apoptosis and enhances cisplatin sensitivity of colorectal carcinoma cells by inhibiting the Akt/mTORC1-mediated Warburg effect.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenyu Yang,Yanchun Wang,Chunhui Tao,Yunhai Li,Shan Cao,Xiqian YangPubMed 35069879
- Frontiers in veterinary science 8:7563752021Intracellular Infection Decreases Milk Protein Synthesis by Preventing Amino Acid Uptake in Bovine Mammary Epithelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuhao Chen,Yuze Ma,Qiang Ji,Xiaoru Yang,Xue Feng,Ruiyuan Yao,Xiaoou Cheng,Tingting Li,Yanfeng Wang,Zhigang WangPubMed 34869729
- Experimental & molecular medicine 53:1207-12172021Histone demethylase KDM4A plays an oncogenic role in nasopharyngeal carcinoma by promoting cell migration and invasion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingyi Zhao,Bingyan Li,Yongxia Ren,Tiansong Liang,Juan Wang,Suna Zhai,Xiqian Zhang,Pengcheng Zhou,Xiangxian Zhang,Yuanyuan Pan,Fangfang Gao,Sulan Zhang,Liming Li,Yongqiang Yang,Xiaoyu Deng,Xiaole Li,Linhui Chen,Daoke Yang,Yingjuan ZhengPubMed 34385569
- Animals : an open access journal from MDPI 11:2020Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Tian,Jiaming Chen,Zhihui Wu,Hanqing Song,Fei Yang,Chang Cui,Fang Chen,Shihai Zhang,Wutai GuanPubMed 33375218
- Cell communication and signaling : CCS 18:1872020Pathogenic effects of inhibition of mTORC1/STAT3 axis facilitates Staphylococcus aureus-induced pyroptosis in human macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuiyuan Yao et. al.PubMed 33256738
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human EIF4EBP1. The exact immunogen used to generate this antibody is proprietary information. Database link Q13541
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Rat | Expected | Tested |
| Chimpanzee | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 - 1/20000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan | Dilution info - | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Alternative names
Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, EIF4EBP1
Recommended products
Rabbit Polyclonal eIF4EBP1 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 37 publications. Immunogen corresponding to Synthetic Peptide within Human EIF4EBP1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human EIF4EBP1. The exact immunogen used to generate this antibody is proprietary information. Database link Q13541
- Specificity
This antibody detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1.
- Concentration
- Purification notes
Affinity purified using the immunising peptideimmobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EIF4EBP1 also known as 4EBP1 or p4EBP1 is an important protein that acts as a translational repressor by binding to eIF4E therefore inhibiting cap-dependent translation. The molecular weight of 4EBP1 is approximately 12 kDa. This protein is ubiquitously expressed in various tissues indicating its widespread involvement in cellular functions. eIF4EBP1 can be detected and quantified using techniques like the eIF4EBP1 ELISA making it a common target in research for its role in translation regulation.
- Biological function summary
EIF4EBP1 plays an important role in regulating cell growth and proliferation by modulating protein synthesis. It is a part of the eIF4F complex which is responsible for the initiation of mRNA translation. When hypophosphorylated eIF4EBP1 binds tightly to eIF4E and prevents the assembly of the active eIF4F complex leading to reduced translation initiation of mRNAs involved in growth and survival. This regulation is important in conditions where cells need to adapt to metabolic stress or external signals.
- Pathways
The mammalian target of rapamycin (mTOR) pathway regulates eIF4EBP1 through phosphorylation. In response to growth signals mTOR phosphorylates eIF4EBP1 causing the release of eIF4E and allowing mRNA translation to proceed. This interaction links eIF4EBP1 to the PI3K/AKT/mTOR signaling pathway which influences cell cycle progression and survival. Related proteins in this pathway include ribosomal protein S6 kinase (S6K1) which is also phosphorylated by mTOR to promote protein synthesis.
- Associated diseases and disorders
Deregulation of eIF4EBP1 has been implicated in cancer and neurological disorders. In cancer hyperactive mTOR signaling can lead to excessive phosphorylation of 4EBP1 decreasing its ability to inhibit eIF4E and enhancing translation of oncogenic proteins. In neurological disorders imbalanced eIF4EBP1 activity can disrupt synaptic plasticity and memory formation. Proteins such as p70S6K which are also part of the mTOR pathway share connections with eIF4EBP1 in these pathological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-eIF4EBP1 antibody (ab2606)
ab2606 at a 1:4000 dilution staining alpha, beta and gamma isoforms of eIF-4EBP1 in 100μg of RIN-m5F (Rat insulinoma cell line) whole cell lysate
after exposure to high (lane 1) and low (lane 2) concentrations of leucine. ab2606 at a 1:4000 dilution staining alpha, beta and gamma isoforms of eIF-4EBP1 in 100μg of RINm5F cell lysate after exposure to high (lane 1) and low (lane 2) concentrations of leucine.All lanes: Western blot - Anti-eIF4EBP1 antibody (ab2606)
Predicted band size: 13 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 antibody (ab2606)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling eIF4EBP1 with ab2606 at 1/20000 (0.05µg/ml). Detection: DAB.
Western blot - Anti-eIF4EBP1 antibody (ab2606)
All lanes: Western blot - Anti-eIF4EBP1 antibody (ab2606) at 1/1000 dilution
Lane 1: Western blot - Recombinant Human eIF4EBP1 protein (Recombinant Human eIF4EBP1 protein ab60007) at 0.01 µg
Lane 2: Western blot - Recombinant Human eIF4EBP1 protein (Recombinant Human eIF4EBP1 protein ab60007) at 0.001 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 13 kDa
Exposure time: 10s
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