AB68187

Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y]

20ul selling size
RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(7 Publications)

Rabbit Recombinant Monoclonal eIF4EBP1 phospho T45 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, EIF4EBP1, Eukaryotic translation initiation factor 4E-binding protein 2, 4E-BP2, eIF4E-binding protein 2, EIF4EBP2, Eukaryotic translation initiation factor 4E-binding protein 3, 4E-BP3, eIF4E-binding protein 3, EIF4EBP3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] (AB68187)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] (AB68187)

Human breast carcinoma tissue stained with ab68187 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] (AB68187)

All lanes:

Western blot - Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] (ab68187)

Lane 1:

293T cell lysate, untreated at 10 µg

Lane 2:

293T cell lysate, treated with FBS at 10 µg

Secondary

All lanes:

HRP conjugated Goat anti-rabbit at 1/2000 dilution

Predicted band size: 13 kDa

Observed band size: 14-19 kDa

false

Carrier free

Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2169Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF4EBP1 eIF4EBP2 and eIF4EBP3 are also known as the eukaryotic translation initiation factor 4E-binding proteins. These proteins regulate translation by binding to eukaryotic translation initiation factor 4E (eIF4E) inhibiting its role in the assembly of the eIF4F complex which is important for cap-dependent translation. eIF4EBP1 weighs approximately 12 kDa eIF4EBP2 around 13 kDa and eIF4EBP3 approximately 13 kDa. These proteins are widely expressed in various tissues with expression levels varying depending on cellular conditions and signaling factors.

Biological function summary

These proteins act as repressors of mRNA translation controlling the initiation phase of protein synthesis. They do so by modulating the availability of eIF4E to form the eIF4F complex when bound preventing the initiation of cap-dependent translation. eIF4EBP proteins are phosphorylated in response to growth factors mitogens and other stimuli which leads to their release from eIF4E allowing translation initiation to proceed. This phosphorylation places eIF4EBP proteins within a complex signaling network that involves mTOR (mechanistic target of rapamycin) signaling.

Pathways

These proteins play an important regulatory role in the mTOR pathway known to control cell growth proliferation and survival. mTOR regulates eIF4EBP phosphorylation status and consequently eIF4E availability impacting protein synthesis rates. In these pathways eIF4EBP proteins interact with proteins like eIF4G which also engage in the translational control machinery. The interplay between these proteins enables cells to respond to nutrient availability and growth signals effectively.

EIF4EBP proteins have links to cancer and metabolic diseases like diabetes. Aberrant mTOR signaling where eIF4EBP proteins are involved can contribute to excessive cell growth and cancer progression as eIF4EBPs regulate translation control. Hyperactive mTOR signaling is also connected to insulin resistance linking eIF4EBP proteins to diabetes. These proteins interact with enzymes like AKT which play roles in these disease processes by further influencing signaling pathways that govern cell metabolism and survival.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex : hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

See full target information EIF4EBP1 pT45

Additional targets

Eukaryotic translation initiation factor 4E-binding protein 3,Eukaryotic translation initiation factor 4E-binding protein 2

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature cardiovascular research 2:1060-1077 PubMed38524149

2024

Translational landscape of direct cardiac reprogramming reveals a role of Ybx1 in repressing cardiac fate acquisition.

Applications

Unspecified application

Species

Unspecified reactive species

Yifang Xie,Qiaozi Wang,Yuchen Yang,David Near,Haofei Wang,Marazzano Colon,Christopher Nguyen,Conor Slattery,Benjamin Keepers,Gregory Farber,Tzu-Wen Wang,Sung-Ho Lee,Yen-Yu Ian Shih,Jiandong Liu,Li Qian

Development (Cambridge, England) 148: PubMed34013332

2021

Regulation of the mammalian maternal-to-embryonic transition by eukaryotic translation initiation factor 4E.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Li,Jianan Tang,Xu Ji,Min-Min Hua,Miao Liu,Lu Chang,Yihua Gu,Changgen Shi,Wuhua Ni,Jing Liu,Hui-Juan Shi,Xuefeng Huang,Christopher O'Neill,Xingliang Jin

Oncotarget 8:20974-20987 PubMed28423496

2017

mTOR inhibitors activate PERK signaling and favor viability of gastrointestinal neuroendocrine cell lines.

Applications

Species

Unspecified reactive species

Patricia Freis,Julien Bollard,Justine Lebeau,Patrick Massoma,Joëlle Fauvre,Cécile Vercherat,Thomas Walter,Serge Manié,Colette Roche,Jean-Yves Scoazec,Carole Ferraro-Peyret

Cancer immunology research 5:363-375 PubMed28351890

2017

Targeting CD47 and Autophagy Elicited Enhanced Antitumor Effects in Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xuyao Zhang,Jiajun Fan,Shaofei Wang,Yubin Li,Yichen Wang,Song Li,Jingyun Luan,Ziyu Wang,Ping Song,Qicheng Chen,Wenzhi Tian,Dianwen Ju

Proceedings of the National Academy of Sciences of 113:11330-11335 PubMed27647922

2016

Normalizing translation through 4E-BP prevents mTOR-driven cortical mislamination and ameliorates aberrant neuron integration.

Applications

IHC-FrFl

Species

Mouse

Tiffany V Lin,Lawrence Hsieh,Tomoki Kimura,Taylor J Malone,Angélique Bordey

Biomaterials 35:7588-97 PubMed24906346

2014

The role of autophagy in the neurotoxicity of cationic PAMAM dendrimers.

Applications

Unspecified application

Species

Unspecified reactive species

Shaofei Wang,Yubin Li,Jiajun Fan,Ziyu Wang,Xian Zeng,Yun Sun,Ping Song,Dianwen Ju

PloS one 8:e72478 PubMed24015249

2013

Autophagy plays a critical role in ChLym-1-induced cytotoxicity of non-hodgkin's lymphoma cells.

Applications

Species

Human

Jiajun Fan,Xian Zeng,Yubin Li,Shaofei Wang,Ziyu Wang,Yun Sun,Hongjian Gao,Guoping Zhang,Meiqing Feng,Dianwen Ju

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] (AB68187)

Western blot - Anti-eIF4EBP1 + eIF4EBP2 + eIF4EBP3 (phospho T45) antibody [EPR2169Y] (AB68187)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (7)

Product promise