AB326706

Anti-eIF4EBP1 (phospho T32) + eIF4EBP2 (phospho T32) + eIF4EBP3 (phospho T46) antibody [SR4378]

Recombinant
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Rabbit Recombinant Monoclonal eIF4EBP1 phospho T46 antibody. Suitable for WB, IHC-P and reacts with Human samples.

View Alternative Names

Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, EIF4EBP1

2 Images

Supplier Data

Western blot - Anti-eIF4EBP1 (phospho T32) + eIF4EBP2 (phospho T32) + eIF4EBP3 (phospho T46) antibody [SR4378] (AB326706)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : 293 treated with 10% FBS for 30min whole cell lysates
Lane 2 : 293 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size : 13-19 kDa
Observed band size : 13-19 kDa
Exposure time : 6 seconds

All lanes:

Western blot - Anti-eIF4EBP1 (phospho T32) + eIF4EBP2 (phospho T32) + eIF4EBP3 (phospho T46) antibody [SR4378] (ab326706) at 1/1000 dilution

Lane 1:

293 treated with 10% FBS for 30min whole cell lysates at 20 µg

Lane 2:

293 whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 13-19 kDa

Observed band size: 13-19 kDa

false

Exposure time: 6s

Supplier Data

Western blot - Anti-eIF4EBP1 (phospho T32) + eIF4EBP2 (phospho T32) + eIF4EBP3 (phospho T46) antibody [SR4378] (AB326706)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : eIF4EBP1 (phospho T32) + eIF4EBP2 (phospho T32) + eIF4EBP3 (phospho T46) knockout Hela cell lysate

Lysates/proteins at 20 µg per lane.

All lanes:

Western blot - Anti-eIF4EBP1 (phospho T32) + eIF4EBP2 (phospho T32) + eIF4EBP3 (phospho T46) antibody [SR4378] (ab326706) at 1/1000 dilution

Lane 1:

Wild-type Hela cell lysate at 20 µg

Lane 2:

eIF4EBP1 (phospho T32) + eIF4EBP2 (phospho T32) + eIF4EBP3 (phospho T46) knockout Hela cell lysate at 20 µg

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR4378

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex : hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

See full target information EIF4EBP1 pT46

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com