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AB47365

Anti-eIF4EBP1 (phospho T36) antibody

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(13 Publications)

Rabbit Polyclonal eIF4EBP1 phospho T36 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human EIF4EBP1 pT36 aa 1-100.

View Alternative Names

Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, EIF4EBP1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-eIF4EBP1 (phospho T36) antibody (AB47365)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-eIF4EBP1 (phospho T36) antibody (AB47365)

ICC/IF image of ab47365 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47365, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 (phospho T36) antibody (AB47365)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 (phospho T36) antibody (AB47365)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47365. Left image without immunising peptide treatment; right image with immunising peptide treatment.

Western blot - Anti-eIF4EBP1 (phospho T36) antibody (AB47365)
  • WB

Unknown

Western blot - Anti-eIF4EBP1 (phospho T36) antibody (AB47365)

All lanes:

Western blot - Anti-eIF4EBP1 (phospho T36) antibody (ab47365)

Lane 1:

MDA-MB-435 cells, treated with EGF (200 ng/ml, 30min)

Lane 2:

MDA-MB-435 cells, untreated

Predicted band size: 13 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human EIF4EBP1 pT36 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q13541

Specificity

ab47365 detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 36. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites.

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF4EBP1 also known as 4EBP1 or p4EBP1 is an important protein that acts as a translational repressor by binding to eIF4E therefore inhibiting cap-dependent translation. The molecular weight of 4EBP1 is approximately 12 kDa. This protein is ubiquitously expressed in various tissues indicating its widespread involvement in cellular functions. eIF4EBP1 can be detected and quantified using techniques like the eIF4EBP1 ELISA making it a common target in research for its role in translation regulation.
Biological function summary

EIF4EBP1 plays an important role in regulating cell growth and proliferation by modulating protein synthesis. It is a part of the eIF4F complex which is responsible for the initiation of mRNA translation. When hypophosphorylated eIF4EBP1 binds tightly to eIF4E and prevents the assembly of the active eIF4F complex leading to reduced translation initiation of mRNAs involved in growth and survival. This regulation is important in conditions where cells need to adapt to metabolic stress or external signals.

Pathways

The mammalian target of rapamycin (mTOR) pathway regulates eIF4EBP1 through phosphorylation. In response to growth signals mTOR phosphorylates eIF4EBP1 causing the release of eIF4E and allowing mRNA translation to proceed. This interaction links eIF4EBP1 to the PI3K/AKT/mTOR signaling pathway which influences cell cycle progression and survival. Related proteins in this pathway include ribosomal protein S6 kinase (S6K1) which is also phosphorylated by mTOR to promote protein synthesis.

Deregulation of eIF4EBP1 has been implicated in cancer and neurological disorders. In cancer hyperactive mTOR signaling can lead to excessive phosphorylation of 4EBP1 decreasing its ability to inhibit eIF4E and enhancing translation of oncogenic proteins. In neurological disorders imbalanced eIF4EBP1 activity can disrupt synaptic plasticity and memory formation. Proteins such as p70S6K which are also part of the mTOR pathway share connections with eIF4EBP1 in these pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex : hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
See full target information EIF4EBP1 pT36
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Emerging microbes & infections 11:519-531 PubMed35060842

2022

hnRNP C modulates MERS-CoV and SARS-CoV-2 replication by governing the expression of a subset of circRNAs and cognitive mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Zhang,Hin Chu,Kenn Ka-Heng Chik,Lei Wen,Huiping Shuai,Dong Yang,Yixin Wang,Yuxin Hou,Terrence Tsz-Tai Yuen,Jian-Piao Cai,Shuofeng Yuan,Feifei Yin,Kwok-Yung Yuen,Jasper Fuk-Woo Chan

Journal of healthcare engineering 2022:2373580 PubMed35035814

2022

circRNA LDLRAD3 Enhances the Malignant Behaviors of NSCLC Cells via the miR-20a-5p-SLC7A5 Axis Activating the mTORC1 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Li,Guangle Qin,Jinyun Du,Peng Yue,Yanling Zhang,Na Hou

Experimental & molecular medicine 53:1207-1217 PubMed34385569

2021

Histone demethylase KDM4A plays an oncogenic role in nasopharyngeal carcinoma by promoting cell migration and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyi Zhao,Bingyan Li,Yongxia Ren,Tiansong Liang,Juan Wang,Suna Zhai,Xiqian Zhang,Pengcheng Zhou,Xiangxian Zhang,Yuanyuan Pan,Fangfang Gao,Sulan Zhang,Liming Li,Yongqiang Yang,Xiaoyu Deng,Xiaole Li,Linhui Chen,Daoke Yang,Yingjuan Zheng

International journal of biological sciences 17:2970-2983 PubMed34345220

2021

MicroRNA-216b targets to potentiate autophagy and apoptosis of breast cancer cells the mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Liu,Ping Ye,Yuanyuan Ye,Baosan Han

Cell cycle (Georgetown, Tex.) 19:3468-3479 PubMed33269647

2020

Suppression of store-operated Ca entry regulated by silencing Orai1 inhibits C6 glioma cell motility via decreasing Pyk2 activity and promoting focal adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Zhu,Bingke Lv,Wenjing Ge,Zhenwen Cui,Kai Zhao,Yugong Feng,Xuejun Yang

Cell transplantation 29:963689720965182 PubMed33028104

2020

LncRNA GASAL1 Interacts with SRSF1 to Regulate Trophoblast Cell Proliferation, Invasion, and Apoptosis Via the mTOR Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Liu,Qing Zhang,Nan Ma

Experimental biology and medicine (Maywood, N.J.) 245:983-993 PubMed32408765

2020

Valsartan ameliorates high glucose-induced peritoneal fibrosis by blocking mTORC1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Liu,Yuan Feng,Cheng Sun,Wei Zhu,Qing-Yan Zhang,Bo Jin,Qiu-Yuan Shao,Yang-Yang Xia,Peng-Fei Xu,Miao Zhang,Chun-Ming Jiang

Cell cycle (Georgetown, Tex.) 19:376-389 PubMed31924125

2020

Role of p75 neurotrophin receptor in neuronal autophagy in intracerebral hemorrhage in rats through the mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Meilei Tian,Yugui Hao

International journal of molecular medicine 43:1939-1950 PubMed30864685

2019

GNG7 silencing promotes the proliferation and differentiation of placental cytotrophoblasts in preeclampsia rats through activation of the mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Si Lai,Yi-Ling Ding

American journal of nephrology 48:357-368 PubMed30423569

2018

Mammalian Target of Rapamycin Complex 1 Activation Disrupts the Low-Density Lipoprotein Receptor Pathway: A Novel Mechanism for Extracellular Matrix Accumulation in Human Peritoneal Mesothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Liu,Wei Zhu,Chun-Ming Jiang,Yuan Feng,Yang-Yang Xia,Qing-Yan Zhang,Peng-Fei Xu,Miao Zhang
View all publications
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