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Rabbit Recombinant Monoclonal eIF4EBP1 phospho T37 antibody. Suitable for Dot, ICC/IF and reacts with Synthetic peptide - Human, Human, Mouse samples.

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Images

Dot Blot - Anti-eIF4EBP1 (phospho T37) antibody [EPR28384-73] (AB322664), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4EBP1 (phospho T37) antibody [EPR28384-73] (AB322664), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4EBP1 (phospho T37) antibody [EPR28384-73] (AB322664), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4EBP1 (phospho T37) antibody [EPR28384-73] (AB322664), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotICC/IFIHC-FrIHC-P
Human
Expected
Tested
Not recommended
Not recommended
Mouse
Expected
Tested
Not recommended
Not recommended
Synthetic peptide - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Synthetic peptide - Human

Dilution info
1/1000
Notes

-

Expected
Expected

Species

Human, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info
1/50
Notes

-

Species

Mouse

Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Synthetic peptide - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Synthetic peptide - Human

Dilution info

-

Notes

-

Target data

Function

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

Alternative names

Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, EIF4EBP1

Rabbit Recombinant Monoclonal eIF4EBP1 phospho T37 antibody. Suitable for Dot, ICC/IF and reacts with Synthetic peptide - Human, Human, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR28384-73

Purification technique

Affinity purification Protein A

Specificity

When tested in dot blot this antibody recognizes eIF4EBP1 phosphorylated at Thr37 and does not cross-react with eIF4EBP1 phosphorylated at Thr46 or Thr36. Antibody may cross-react with eIF4EBP2 and eIF4EBP3 when phosphorylated at equivalent sites.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Dot Blot - Anti-eIF4EBP1 (phospho T37) antibody [EPR28384-73] (ab322664), expandable thumbnail

    Dot Blot - Anti-eIF4EBP1 (phospho T37) antibody [EPR28384-73] (ab322664)

    Dot blot analysis of eIF4EBP1 (phospho T37) using ab322664 at 1000 (0.511 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Lane1: Human eIF4EBP1 (phospho T37) peptide

    Lane2: Human eIF4EBP1 non-phospho control peptide for lane 1

    Lane3: Human eIF4EBP1 (phospho T46) peptide

    Lane4: Human eIF4EBP1 (phospho T36) peptide

    Lane5: Human eIF4EBP2 (phospho T37) peptide

    Lane6: Human eIF4EBP3 (phospho T23) peptide

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    When tested in dot blot this antibody recognizes eIF4EBP1 phosphorylated at Thr37 and does not cross-react with eIF4EBP1 phosphorylated at Thr46 or Thr36. Antibody may cross-react with eIF4EBP2 and eIF4EBP3 when phosphorylated at equivalent sites.

    All lanes: Dot Blot - Anti-eIF4EBP1 (phospho T37) antibody [EPR28384-73] (ab322664) at 1/1000 dilution

    Lane 1: Human eIF4EBP1 (phospho T37) peptide a

    Lane 2: Human eIF4EBP1 non-phospho control peptide for lane 1

    Lane 3: Human eIF4EBP1 (phospho T46) peptide b

    Lane 4: Human eIF4EBP1 (phospho T36) peptide c

    Lane 5: Human eIF4EBP2 (phospho T37) peptide d

    Lane 6: Human eIF4EBP3 (phospho T23) peptide e

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4EBP1 (phospho T37) antibody [EPR28384-73] (ab322664), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-eIF4EBP1 (phospho T37) antibody [EPR28384-73] (ab322664)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labelling eIF4EBP1 (phospho T37) with ab322664 at 1/50 (10.22 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

    Confocal image showing cytoplasmic and nuclear staining in F9 cell line (shown in green), the signal both decreased after LY294002 (50 uM) for 2h or phosphatase treatment at 37℃ for 2h. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/ 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ 1000 2ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4EBP1 (phospho T37) antibody [EPR28384-73] (ab322664), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-eIF4EBP1 (phospho T37) antibody [EPR28384-73] (ab322664)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized LNCaP (human prostate carcinoma epithelial cell) cells labelling eIF4EBP1 (phospho T37) with ab322664 at 1/50 (10.22 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ 1000 2ug/ml dilution (Green).

    Confocal image showing cytoplasmic and nuclear staining in LNCaP cell line (shown in green), the signal both decreased after LY294002 (50 uM) for 2h or phosphatase treatment at 37℃ for 2h. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/ 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ 1000 2ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4EBP1 (phospho T37) antibody [EPR28384-73] (ab322664), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-eIF4EBP1 (phospho T37) antibody [EPR28384-73] (ab322664)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EIF4EBP1 KO HAP1 (EIF4EBP1 knockout human chronic myelogenous leukemia near-haploid cell) cells labelling eIF4EBP1 (phospho T37) with ab322664 at 1/50 (10.22 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

    Confocal image showing cytoplasmic and nuclear staining in parental HAP1 cell line (shown in green), the signal decreased after LY294002 (50 uM) for 2 h. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ 1000 2ug/ml dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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