JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB75767

Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y]

Be the first to review this product! Submit a review

|

(28 Publications)

Rabbit Recombinant Monoclonal eIF4EBP1 phospho T37 antibody. Suitable for WB and reacts with Human samples. Cited in 28 publications.

View Alternative Names

Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, EIF4EBP1

1 Images
Western blot - Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] (AB75767)
  • WB

Unknown

Western blot - Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] (AB75767)

All lanes:

Western blot - Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] (ab75767) at 1/2000 dilution

Lane 1:

293 cell lysate, untreated at 10 µg

Lane 2:

293 cell lysate, treated with insulin at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 13 kDa

Observed band size: 17 kDa

false

  • Carrier free

    Anti-eIF4EBP1 (phospho T37) antibody [EPR729(2)Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR729(2)Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB111421

Anti-eIF4EBP1 (phospho T70) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 (phospho T70) antibody (AB111421)

  • 0
  • 0
Primary Antibodies

AB47365

Anti-eIF4EBP1 (phospho T36) antibody

Immunocytochemistry/ Immunofluorescence - Anti-eIF4EBP1 (phospho T36) antibody (AB47365)

  • 0
  • 0
Primary Antibodies

AB27792

Anti-eIF4EBP1 (phospho T46) antibody

Immunocytochemistry/ Immunofluorescence - Anti-eIF4EBP1 (phospho T46) antibody (AB27792)

  • 0
  • 0
Primary Antibodies

AB75831

Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A]

20ul selling size|RabMAb|Recombinant|Trial Size|Trial Size

Western blot - Anti-eIF4EBP1 (phospho T70) antibody [EPR654(2)A] (AB75831)

  • 0
  • 0
Primary Antibodies

AB173370

Anti-eIF4EBP1 antibody [Y329] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-eIF4EBP1 antibody [Y329] - BSA and Azide free (AB173370)

  • 0
  • 0
Primary Antibodies

AB2606

Anti-eIF4EBP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 antibody (AB2606)

  • 5
  • 4
Primary Antibodies

AB32130

Anti-eIF4EBP1 antibody [Y330]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-eIF4EBP1 antibody [Y330] (AB32130)

  • 0
  • 0

Complementary Products

Assay Kits

AB289651

Human eIF4eBP1 ELISA Kit

SimpleStep|Recombinant

Sandwich ELISA - Human eIF4eBP1 ELISA Kit (AB289651)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB32529

Anti-S6K1 antibody [E343]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 antibody [E343] (AB32529)

  • 1
  • 1
Primary Antibodies

AB109268

Anti-mTOR (phospho S2448) antibody [EPR426(2)]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Frozen sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (AB109268)

  • 4
  • 6
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF4EBP1 also known as 4EBP1 or p4EBP1 is an important protein that acts as a translational repressor by binding to eIF4E therefore inhibiting cap-dependent translation. The molecular weight of 4EBP1 is approximately 12 kDa. This protein is ubiquitously expressed in various tissues indicating its widespread involvement in cellular functions. eIF4EBP1 can be detected and quantified using techniques like the eIF4EBP1 ELISA making it a common target in research for its role in translation regulation.
Biological function summary

EIF4EBP1 plays an important role in regulating cell growth and proliferation by modulating protein synthesis. It is a part of the eIF4F complex which is responsible for the initiation of mRNA translation. When hypophosphorylated eIF4EBP1 binds tightly to eIF4E and prevents the assembly of the active eIF4F complex leading to reduced translation initiation of mRNAs involved in growth and survival. This regulation is important in conditions where cells need to adapt to metabolic stress or external signals.

Pathways

The mammalian target of rapamycin (mTOR) pathway regulates eIF4EBP1 through phosphorylation. In response to growth signals mTOR phosphorylates eIF4EBP1 causing the release of eIF4E and allowing mRNA translation to proceed. This interaction links eIF4EBP1 to the PI3K/AKT/mTOR signaling pathway which influences cell cycle progression and survival. Related proteins in this pathway include ribosomal protein S6 kinase (S6K1) which is also phosphorylated by mTOR to promote protein synthesis.

Deregulation of eIF4EBP1 has been implicated in cancer and neurological disorders. In cancer hyperactive mTOR signaling can lead to excessive phosphorylation of 4EBP1 decreasing its ability to inhibit eIF4E and enhancing translation of oncogenic proteins. In neurological disorders imbalanced eIF4EBP1 activity can disrupt synaptic plasticity and memory formation. Proteins such as p70S6K which are also part of the mTOR pathway share connections with eIF4EBP1 in these pathological conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex : hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
See full target information EIF4EBP1 pT37
style
left-column

Publications (28)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 23:e3003362 PubMed40934285

2025

The mTORC2 subunit RICTOR drives breast cancer progression by promoting ganglioside biosynthesis through transcriptional and epigenetic mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Nafees Ansari,Somesh K Jha,Ali Khan,Kajal Rajput,Nishant Pandey,Dolly Jain,Rajeshwari Tripathi,Nihal Medatwal,Pankaj Sharma,Sudeshna Datta,Animesh Kar,Trishna Pani,Sk Asif Ali,Kaushavi Cholke,Kajal Rana,Valiya P Snijesh,Geetashree Mukherjee,Suryanarayana V S Deo,Soumen Basak,Ashutosh Mishra,Jyothi S Prabhu,Arnab Mukhopadhyay,Avinash Bajaj,Ujjaini Dasgupta

Environmental toxicology 39:421-434 PubMed37792549

2023

Spleen tyrosine kinase facilitates the progression of papillary thyroid cancer regulated by the hsa_circ_0006417/miR-377-3p axis.

Applications

Unspecified application

Species

Unspecified reactive species

Guangmou Tan,Shiyang Zheng,Boxuan Zhou,Zhaohong Mo,Qiong Zhang,Donghui Zhang,Aimin Li,Xinhui Liu

Experimental and therapeutic medicine 24:613 PubMed36160908

2022

TIPE2 protects cardiomyocytes from ischemia-reperfusion-induced apoptosis by decreasing cell autophagy via the mTORC1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Gong Cheng,Xiaoyan Huang,Penghua You,Panpan Feng,Shuo Jia,Ji Zhang,Hongjun You,Fengjun Chang

FEBS open bio 12:1761-1770 PubMed35748097

2022

PF-06409577 inhibits renal cyst progression by concurrently inhibiting the mTOR pathway and CFTR channel activity.

Applications

Unspecified application

Species

Unspecified reactive species

Limin Su,Haoxing Yuan,Haoran Zhang,Ruoqi Wang,Kequan Fu,Long Yin,Ying Ren,Hongli Liu,Qian Fang,Junqi Wang,Dong Guo

Frontiers in cell and developmental biology 10:800925 PubMed35419359

2022

SLC1A5 Prefers to Play as an Accomplice Rather Than an Opponent in Pancreatic Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fangshi Xu,Hai Wang,Honghong Pei,Zhengliang Zhang,Liangliang Liu,Long Tang,Shuang Wang,Bin-Cheng Ren

Neurology international 14:212-244 PubMed35225888

2022

Hypomyelinating Leukodystrophy 8 (HLD8)-Associated Mutation of POLR3B Leads to Defective Oligodendroglial Morphological Differentiation Whose Effect Is Reversed by Ibuprofen.

Applications

Unspecified application

Species

Unspecified reactive species

Sui Sawaguchi,Rimi Suzuki,Hiroaki Oizumi,Katsuya Ohbuchi,Kazushige Mizoguchi,Masahiro Yamamoto,Yuki Miyamoto,Junji Yamauchi

iScience 25:103607 PubMed35005552

2022

Integrative proteo-transcriptomic and immunophenotyping signatures of HIV-1 elite control phenotype: A cross-talk between glycolysis and HIF signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Svensson Akusjärvi,Anoop T Ambikan,Shuba Krishnan,Soham Gupta,Maike Sperk,Ákos Végvári,Flora Mikaeloff,Katie Healy,Jan Vesterbacka,Piotr Nowak,Anders Sönnerborg,Ujjwal Neogi

Neurology international 14:11-33 PubMed35076634

2021

Hypomyelinating Leukodystrophy 7 (HLD7)-Associated Mutation of POLR3A Is Related to Defective Oligodendroglial Cell Differentiation, Which Is Ameliorated by Ibuprofen.

Applications

Unspecified application

Species

Unspecified reactive species

Sui Sawaguchi,Kenji Tago,Hiroaki Oizumi,Katsuya Ohbuchi,Masahiro Yamamoto,Kazushige Mizoguchi,Yuki Miyamoto,Junji Yamauchi

Scientific reports 11:24005 PubMed34907287

2021

Spontaneous formation of neutrophil extracellular traps is associated with autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Guo,Fei Gao,Xin Wang,Zhenzhen Pan,Qian Wang,Shiyao Xu,Shanshan Pan,Ling Li,Deyu Zhao,Jun Qian

Animal cells and systems 25:283-295 PubMed34745435

2021

Se improves GPX4 expression and SOD activity to alleviate heat-stress-induced ferroptosis-like death in goat mammary epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Liu,Manjiang Wang,Ning Gong,Peng Tian,Hongxia Deng
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com