AB278686

Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5]

20ul selling size
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal eIF4EBP1 phospho T37 + T46 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human EIF4EBP1 phospho T37 + T46.

View Alternative Names

Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, EIF4EBP1

5 Images

Flow Cytometry - Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (AB278686)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (AB278686)

Peptide blocking flow cytometric analysis of Jurkat cells LY294002 plus wortmannin plus U0126 LWU) treated cells stained with secondary antibody as negative control (light blue) or treated with LWU (red) or treated with FBS (green) or LUW and blocked with phospho-peptide (black) or FBS and blocked with phospho peptide (gold) or LUW and blocked with non-phospho peptide (dark blue) or FBS and blocked with non-phospho peptide (purple) using ab278686 at 0.1μg/mL.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (AB278686)

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or treated with LY294002 + U0126 + wortmanmin (red) or with FBS (green) using 0.1 ug/mL of ab278686.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (AB278686)

Flow cytometric analysis of C6 cells untreated cells stained with secondary antibody as negative control (blue) or untreated (red) or treated with UV and TPA (green) and stained using ab278686 at 0.1µg/mL.

Supplier Data

Western blot - Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (AB278686)

All lanes:

Western blot - Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (ab278686) at 0.001 µg/mL

Lane 1:

K562 cell extract untreated

Lane 2:

K562 cell extract treated with imatinib

Predicted band size: 13 kDa

false

Supplier Data

Western blot - Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (AB278686)

All lanes:

Western blot - Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (ab278686) at 0.001 µg/mL

Lane 1:

NIH3T3 cell extract untreated

Lane 2:

NIH3T3 cell extract treated with PDGF

Predicted band size: 13 kDa

false

660 APC

APC Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5]
578 PE

PE Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5]
519 FITC

FITC Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

4EB1T37T46-A5

Isotype

IgG

Light chain type

kappa

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, Flow Cyt, WB

applications

Immunogen

Synthetic Peptide within Human EIF4EBP1 phospho T37 + T46. The exact immunogen used to generate this antibody is proprietary information.

Q13541

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.001 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.001 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EIF4EBP1 also known as 4EBP1 or p4EBP1 is an important protein that acts as a translational repressor by binding to eIF4E therefore inhibiting cap-dependent translation. The molecular weight of 4EBP1 is approximately 12 kDa. This protein is ubiquitously expressed in various tissues indicating its widespread involvement in cellular functions. eIF4EBP1 can be detected and quantified using techniques like the eIF4EBP1 ELISA making it a common target in research for its role in translation regulation.

Biological function summary

EIF4EBP1 plays an important role in regulating cell growth and proliferation by modulating protein synthesis. It is a part of the eIF4F complex which is responsible for the initiation of mRNA translation. When hypophosphorylated eIF4EBP1 binds tightly to eIF4E and prevents the assembly of the active eIF4F complex leading to reduced translation initiation of mRNAs involved in growth and survival. This regulation is important in conditions where cells need to adapt to metabolic stress or external signals.

Pathways

The mammalian target of rapamycin (mTOR) pathway regulates eIF4EBP1 through phosphorylation. In response to growth signals mTOR phosphorylates eIF4EBP1 causing the release of eIF4E and allowing mRNA translation to proceed. This interaction links eIF4EBP1 to the PI3K/AKT/mTOR signaling pathway which influences cell cycle progression and survival. Related proteins in this pathway include ribosomal protein S6 kinase (S6K1) which is also phosphorylated by mTOR to promote protein synthesis.

Deregulation of eIF4EBP1 has been implicated in cancer and neurological disorders. In cancer hyperactive mTOR signaling can lead to excessive phosphorylation of 4EBP1 decreasing its ability to inhibit eIF4E and enhancing translation of oncogenic proteins. In neurological disorders imbalanced eIF4EBP1 activity can disrupt synaptic plasticity and memory formation. Proteins such as p70S6K which are also part of the mTOR pathway share connections with eIF4EBP1 in these pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex : hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

See full target information EIF4EBP1 phospho T37 + T46

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:122 PubMed40158101

2025

The m6A reader IGF2BP2 promotes pancreatic cancer progression through the m6A-SLC1A5-mTORC1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Pu,Yuting Wu,Weiguo Long,Xinyu Sun,Xiao Yuan,Deqiang Wang,Xu Wang,Min Xu

Autophagy 20:505-524 PubMed37772772

2023

Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Li Xia,Tiejian Nie,Fangfang Lu,Lu Huang,Xiaolong Shi,Dongni Ren,Jianjun Lu,Xiaobin Li,Tuo Xu,Bozhou Cui,Qing Wang,Guodong Gao,Qian Yang

Neural regeneration research 19:642-649 PubMed37721296

2023

Treatment with β-sitosterol ameliorates the effects of cerebral ischemia/reperfusion injury by suppressing cholesterol overload, endoplasmic reticulum stress, and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuling Tang,Tao Yan,Saiying Wang,Qingqing Liu,Qi Yang,Yongqiang Zhang,Yujiao Li,Yumei Wu,Shuibing Liu,Yulong Ma,Le Yang

Pain research & management 2023:8870256 PubMed37397163

2023

Scraping Therapy Improved Muscle Regeneration through Regulating GLUT4/Glycolytic and AMPK/mTOR/4EBP1 Pathways in Rats with Lumbar Multifidus Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Zou,Juan Du,Qiwen Xuan,Yajing Wang,Zixiao Wang,Wen Zhang,Lianghua Wang,Wei Gu

Experimental and therapeutic medicine 21:581 PubMed33850553

2021

Metformin prevents PFKFB3-related aerobic glycolysis from enhancing collagen synthesis in lung fibroblasts by regulating AMPK/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ci-Jun Tang,Ji Xu,Hai-Yan Ye,Xue-Bin Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5]

Flow Cytometry - Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (AB278686)

Flow Cytometry - Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (AB278686)

Flow Cytometry - Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (AB278686)

Western blot - Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (AB278686)

Western blot - Anti-eIF4EBP1 (phospho T37 + T46) antibody [4EB1T37T46-A5] (AB278686)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise